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  • DOTAP Liposomal Transfection Reagent From Roche Applied Science

    DOTAP Liposomal Transfection Reagent From Roche Applied Science

    Roche Applied Science
    Tuesday, July 17, 2007
    If looking for a cationic liposome mediated transfection reagent, consider DOTAP from Roche Applied Sciences. This transfection reagent is able to transfect all types of negatively charged molecules ... read more
  • DOTAP Transfection Reagent From Roche

    DOTAP Transfection Reagent From Roche

    Roche Applied Science
    Friday, July 13, 2007
    DOTAP is a cationic liposomal reagent for transfection (lipofection) of eukaryotic cells with DNA or RNA; I have even heard about researchers using this reagent to introduce new proteins... read more
  • Cell Proliferation Kit II (XTT) From Roche

    Cell Proliferation Kit II (XTT) From Roche

    Roche Applied Science
    Thursday, June 28, 2007
    The XTT Cell Proliferation Kit II analyzes the number of viable cells in a culture based on the cleavage of the yellow tetrazolium salt XTT. XTT is cleaved to formazan in viable (metabolically active... read more
  • Cytotoxicity Detection Kit (LDH) From Roche Applied Science

    Cytotoxicity Detection Kit (LDH) From Roche Applied Science

    Roche Applied Science
    Wednesday, June 20, 2007
    The Roche LDH Cytotoxicity Detection Kit is designed to be a simple method to directly quantify cell death in culture. The assay is based on the measurement of lactate dehydrogenase (LDH) released ... read more
  • Expand High FidelityPLUS PCR System, dNTPack from Roche Applied Sciences

    Expand High FidelityPLUS PCR System, dNTPack from Roche Applied Sciences

    Roche Applied Science
    Wednesday, June 13, 2007
    The Expand High FidelityPLUS PCR System, dNTPack from Roche Applied Sciences is a PCR kit which includes all reagents necessary to accurately amplify DNA targets ≤5 kb with high sensitivity: a ... read more
  • Cell Proliferation Reagent WST-1 From Roche Applied Science

    Cell Proliferation Reagent WST-1 From Roche Applied Science

    Roche Applied Science
    Monday, April 23, 2007
    The Cell Proliferation Reagent WST-1 by Roche Applied Science provides a colorimetric assay for the quantification of adherent or suspension cell viability and proliferation. The reagent is a sterile... read more
  • Cellular DNA Flow Cytometric Analysis Kit from Roche

    Cellular DNA Flow Cytometric Analysis Kit from Roche

    Roche Applied Science
    Friday, April 13, 2007
    Analysis of cell cycle kinetics is a critical parameter for a wide range of research areas; accuracy and consistency is of utmost importance. The Cellular DNA Flow Cytometric Analysis Kit provides a ... read more
  • Cell Proliferation Reagent WST-1 From Roche Applied Science

    Cell Proliferation Reagent WST-1 From Roche Applied Science

    Roche Applied Science
    Wednesday, April 04, 2007
    Roche’s WST-1 cell proliferation reagent is a simple, colorimetric assay designed to measure the relative proliferation rates of cells in culture. The assay principle is based on the conversion of the... read more
  • DIG RNA Labeling Mix From Roche

    DIG RNA Labeling Mix From Roche

    Roche Applied Science
    Monday, February 12, 2007
    We have routinely used the Roche DIG-UTP RNA Labeling Mix to synthesize single stranded, DIG-labeled RNA probes for in-situ hybridization (ISH) in zebrafish embryos. Digoxigenin (DIG) is a small ... read more
  • The DIG System From Roche

    The DIG System From Roche

    Roche Applied Science
    Tuesday, January 02, 2007
    The first Northern blot I did in my life was using 32P-labeled probes, and I still remember how nervous I was hearing the buzzing of the Geiger counter and how inconvenient it was to perform all of ... read more
  • LightCycler® FastStart DNA Master SYBR® Green I From Roche Applied Science

    LightCycler® FastStart DNA Master SYBR® Green I From Roche Applied Science

    Roche Applied Science
    Friday, December 15, 2006
    About 6 months ago, our lab decided to move to real-time PCR system, also known as quantitative PCR (qPCR). Our research department has the Roche LightCycler®® 2.0, and I began to set-up the system ... read more
  • PCR DIG Probe Synthesis Kit From Roche

    PCR DIG Probe Synthesis Kit From Roche

    Roche Applied Science
    Thursday, November 16, 2006
    The PCR DIG Probe Synthesis Kit is a non-radioactive system using digoxigenin (DIG), a steroid hapten, to label DNA probes for Southern hybridization. The labeled dUTP can be easily incorporated by ... read more
  • CAT ELISA Kit From Roche Applied Science

    CAT ELISA Kit From Roche Applied Science

    Roche Applied Science
    Wednesday, November 15, 2006
    Chloramphenicol acetyltransferase (CAT) assays are a fast, easy and accurate method of measuring gene promoter activity in vitro. As the CAT gene is not expressed in eukaryotic cells, by inserting a ... read more
  • Cryo-Star HM 560 Cryostat From MICROM International

    Cryo-Star HM 560 Cryostat From MICROM International

    MICROM International GmbH
    Friday, November 10, 2006
    The Microm HM560 is a cryostat with electronic temperature control down to -35°C. It also offers fast freezing with Peltier down to -55°C and a spacious, easy-to-clean cooling chamber. We chose this ... read more
  • FuGene® 6 Transfection Reagent From Promega

    FuGene® 6 Transfection Reagent From Promega

    Roche Applied Science
    Wednesday, October 25, 2006
    Transfection of cloned genes into mammalian cells is a powerful tool for biologists to study a specific gene. Many chemicals/methods can be used to introduce foreign DNA into cultured cells including... read more
  • Expand High Fidelity Plus PCR System From Roche Applied Science

    Expand High Fidelity Plus PCR System From Roche Applied Science

    Roche Applied Science
    Thursday, October 12, 2006
    The Roche Expand High FidelityPLUS PCR System contains a master mix enzyme blend of Taq DNA polymerase and a proofreading enzyme that lacks polymerase activity. These enzymes are designed to increase... read more
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