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  • Fast and inexpensive sequencing service

    Fast and inexpensive sequencing service

    Thursday, January 22, 2015
    I have used the services from GENEWIZ several times to perform sequencing of purified plasmid DNA. The sample preparation is easy; they only require 500-1000ng of DNA in 10uL volume with another 5uL ... read more
  • Nice LC3B antibody for tougher protein

    Nice LC3B antibody for tougher protein

    Cell Signaling Technology
    Tuesday, January 20, 2015
    This antibody was used in a study that investigated the role of LC3-B in autophagy. Several LC3-B antibodies were tested in this study for various applications; the antibody reviewed here (2775) gave ... read more
  • Good Antibody, Needs a lot to detect Western bands

    Good Antibody, Needs a lot to detect western bands

    Cell Signaling Technology
    Tuesday, January 20, 2015
    This product is one of few phospho antibodies on the market for Pin1. It comes as 50ul but about a 1:100 to 1:500 dilution is necessary to detect phosphorylation reliably. The blot is often dirty, ... read more
  • Works for chemiluminescence but not well for fluorescence - Unattributed Review*

    Works for chemiluminescence but not well for fluorescence - Unattributed Review*

    Cell Signaling Technology
    Monday, January 19, 2015
    I wanted to measure GluR2 protein levels in brain tissue homogenates via immunoblot. The bands visible in the blot were not crisp and clear, but still at the appropriate weight. No side bands were ... read more
  • Turtle CREB Antibody for chromatin immunoprecipitation

    Turtle CREB Antibody for chromatin immunoprecipitation

    Cell Signaling Technology
    Thursday, January 08, 2015
    We are investigating the regulatory mechanisms controlling BDNF expression in an in vitro model of eyeblink classical conditioning, we analyzed the transcription factors and other signaling proteins ... read more
  • LC3A/B human antibody, one of the best autophagy markers!

    LC3A/B human antibody, one of the best autophagy markers!

    Cell Signaling Technology
    Thursday, January 08, 2015
    The CST LC3A/B antibody is one of the most widely used autophagy markers on the market. It is usable in immunoblot assays in which the detection of the slightly lower band (also called LC3II) ... read more
  • IF of Chk1 pS345

    IF of Chk1 pS345

    Cell Signaling Technology
    Monday, January 05, 2015
    Chk1 is phosphorylated on Ser 317 and Ser 345 by ATR protein kinases after DNA damage. Phosphorylation of Chk1 at S317 and S345 is a hallmark of activation of the cell cycle checkpoint. The UV-induced... read more
  • Western blot of Phospho Chk1 pS317

    Western blot of Phospho Chk1 pS317

    Cell Signaling Technology
    Monday, December 29, 2014
    Chk1 is an effector kinase that acts downstream of ATR in response to DNA damage and replication stress. Phosphorylation of Chk1 at S317 and 345 are hallmarks of the activation cell cycle checkpoint. ... read more
  • Western blotting of anti-Chk1pS345

    Western blotting of anti-Chk1pS345

    Cell Signaling Technology
    Friday, December 26, 2014
    Chk1 is a key effector kinase that controls cell cycle progression after DNA damage. Activation of Chk1 relies on the phosphorylation of Ser 317 and Ser 345 by ATR. Here I tested the UV-induced ... read more
  • Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (for Western Blot) from Cell Signaling Technology

    Phospho-S6 Ribosomal Protein (Ser235/236) Antibody (for Western Blot) from Cell Signaling Technology

    Cell Signaling Technology
    Tuesday, December 16, 2014
    This antibody was used in a study that investigated the role of Phospho- S6 Ribosomal Protein (Ser235/236) in fibroblast cells from a neonatal human and a patient with de novo cardiovascular diseases... read more
  • Cleaved PARP antibody

    Cleaved PARP antibody

    Cell Signaling Technology
    Thursday, December 11, 2014
    Poly(ADP-ribose) polymerase (PARP) is a nuclear DNA binding protein which can detect DNA strand breaks and is involved in base excision repair. Once PARP is cleaved by caspase during apoptosis, its ... read more
  • Great Phospho-Chk1 antibody

    Great Phospho-Chk1 antibody

    Cell Signaling Technology
    Friday, December 05, 2014
    Phosphorylation of Chk1 at S317 is a hallmark of the activation of cell cycle checkpoint. The goal of this experiment is to examine whether after UV irradiation Phospho-Chk1 (S317) can be detected in ... read more
  • Very potent antibody for pERK flow staining

    Very potent antibody for pERK flow staining

    Cell Signaling Technology
    Monday, November 24, 2014
    Upon T-cell receptor activation, T cells turn on MAPK pathways including the ERK (p44/42) activation, which is critical for T cell activation and further differentiation of effector functions. Given ... read more
  • Phospho-p38 - Western Blot

    Phospho-p38 - Western Blot

    Cell Signaling Technology
    Friday, November 21, 2014
    phospho- p38 protein expression was characterized via Western blotting using the Phospho-p38 MAPK (Thr180/Tyr182) Antibody from Cell Signaling Technology (1:500) read more
  • Phospho Akt protein expression analysis via Western blotting

    Phospho Akt protein expression analysis via Western blotting

    Cell Signaling Technology
    Friday, November 14, 2014
    Phospho Akt/PKB protein expression was characterized via Western blot using the Phospho-Akt (Ser473) Mouse mAb (1:1000). read more
  • Akt/PKB protein expression analysis via Western blotting

    Akt/PKB protein expression analysis via Western blotting

    Cell Signaling Technology
    Thursday, November 13, 2014
    Akt/PKB protein expression was analyzed via Western blotting with Akt Mouse mAb (1:1000) in murine cardiomyocytes read more
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