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  • Excellent Human CD45 Antibody

    Excellent Human CD45 Antibody

    Friday, May 01, 2015
    Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. I have used this antibody to separate human cells from the spleen of these ... read more
  • Very Good Anti-Human HLA-DR  antibody

    Very Good Anti-Human HLA-DR antibody

    Wednesday, April 29, 2015
    Human PBMC reconstituted NOD-scid IL2r?cnull mice (NSG mice), were sacrificed and single cell suspensions from spleen was used for flow cytometry. This antibody was used for detecting human HLA-DR ... read more
  • Very Good Anti-Human PD-1  antibody

    Very Good Anti-Human PD-1 antibody

    Tuesday, April 28, 2015
    Human PBMC reconstituted NOD-scid IL2r?cnull mice (NSG mice), were sacrificed and single cell suspensions from spleen was used for flow cytometry. This antibody was used for detecting PD-1 expression ... read more
  • Very Good Anti-Human CD8 antibody

    Very Good Anti-Human CD8 antibody

    Monday, April 27, 2015
    NOD-scid IL2rgammac-null mice (NSG mice) reconstitute with human PBMCs were sacrificed and single cell suspensions prepared from spleen was used for flow cytometric analysis. The cells were stained ... read more
  • Very Good Anti-Human CD45  antibody

    Very Good Anti-Human CD45 antibody

    Thursday, April 23, 2015
    Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. I have used this antibody to separate human cells from the spleen of these ... read more
  • Immunoprecipitaiton (IP) Using ITCH Antibody

    Immunoprecipitaiton (IP) Using ITCH Antibody

    Monday, March 30, 2015
    Immunoprecipitation of ITCH from KB cell extracts using ITCH (BD Biosciences: Cat# 611199) mouse mAb (lane 1) or mouse IgG isotype Control (lane 2) was performed. Western blot analysis was performed ... read more
  • Excellent FACS machine for multiple color staining

    Excellent FACS machine for multiple color staining

    Thursday, March 19, 2015
    We routinely use the BD LSR II for multiple color staining, generally around 10 colors at the same time. read more
  • Th1/Th2/Th17 CBA for cytokine production analysis

    Th1/Th2/Th17 CBA for cytokine production analysis

    Wednesday, March 04, 2015
    The aim of my research is to determine the immune modulating capabilities of drug treatments in Parkinson's disease mouse models. I look at the ability of drug therapies to modulate the T cell ... read more
  • The use of T75 flasks in culture of BV-2 cells

    The use of T75 flasks in culture of BV-2 cells

    Tuesday, March 03, 2015
    The microglial cell line, BV-2, is cultured every 3-4 days in new medium in a T75 flask. These T75 flasks work great for the culturing of BV-2 cells. With the use of the T75 flask I am able to ... read more
  • Use of 18G Precision Glide Needle In Performing Single Cell Suspension

    Use of 18G Precision Glide Needle In Performing Single Cell Suspension

    Friday, February 27, 2015
    After spleens have been removed from mice, a single cell suspension of splenocytes is performed using a 18G Precision Glide Needle. read more
  • BD Pharmigen CD11c HL3 Clone

    BD Pharmigen CD11c HL3 Clone

    Friday, February 06, 2015
    Our experiments assess the tendencies of environmental pollutants to affect dendritic cells' protein expression. We incubated Bone-Marrow Derived DCs with pollutant and collected data via flow ... read more
  • Very Good Th1/Th2/Th17 Kit

    Very Good Th1/Th2/Th17 Kit

    Wednesday, January 21, 2015
    This kit is very good for detecting human Th1/Th2/Th17 cytokines from the serum as well as cell culture supernatant. read more
  • PE-CF594 anti mouse RORgamma-t for Th17 cells

    PE-CF594 anti mouse RORgamma-t for Th17 cells

    Tuesday, January 13, 2015
    We transfer wild type CD4 T cells to Rag-/- mice to establish a Colitis model and then look at the phenotype of these transferred CD4 T cells. RORgamma-t is required for the differentiation of Th17 ... read more
  • Review of Mouse IFN gamma ELISPOT kit

    Review of Mouse IFN gamma ELISPOT kit

    Friday, December 12, 2014
    The aim of the research is to monitor IFN gamma production in mouse splenocytes in response to vaccination with Newcastle disease virus recombinants expressing HIV gp160 and Gag proteins. I selected ... read more
  • BUV395 amazing addition in the multicolor panel

    BUV395 amazing addition in the multicolor panel

    Thursday, December 11, 2014
    563793 and 563557 BUV provides a new addition in the multi-color flowcytometry panel. I have tried both Anti-mouse CD19 and CD45R (B220) with this channel and both work extremely well . As ... read more
  • Ab used for staining of mouse naive T cells from spleen

    Ab used for staining of mouse naive T cells from spleen

    Friday, December 05, 2014
    Here I am looking at two parameters at the same time (CD25 PE X-axis and Foxp3e450 Y-Axis). This ab was used to stain mouse naive CD4 T cells from the spleen CD3+CD4+CD25-CD44 low CD62L high. ... read more
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