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  • The Mammalian Gene Collection

    The Mammalian Gene Collection

    Mammalian Gene Collection
    Wednesday, August 10, 2005
    I recently had the opportunity to take advantage of a fabulous web-based resource called The Mammalian Gene Collection (http://mgc.nci.nih.gov/). In simplest terms, the MGC is a searchable collection ... read more
  • Invitrogen’s Optifect Transfection Reagent

    Invitrogen’s Optifect Transfection Reagent

    Life Technologies
    Thursday, November 03, 2005
    The objective of lipid cationic delivery is to obtain the highest efficiency possible, while minimizing the level of cell death. Most of the reagents used require plating of the cells at a high ... read more
  • Effectene™ Transfection Reagent From Qiagen

    Effectene™ Transfection Reagent From Qiagen

    Wednesday, November 08, 2006
    Transfection reagents and protocols have become very widespread and utilized in many aspects of DNA research. There are many applications that require the introduction of vectors and expression ... read more
  • Lipofectamine™ 2000 from Invitrogen

    Lipofectamine™ 2000 from Invitrogen

    Life Technologies
    Friday, December 01, 2006
    Transfection reagents are big business. The ability to express exogenous genes within eukaryotic cells is very popular and companies are falling over themselves marketing these products, claiming to ... read more
  • Lipofectamine™ 2000 Transfection Reagent From Invitrogen

    Lipofectamine™ 2000 Transfection Reagent From Invitrogen

    Life Technologies
    Tuesday, March 27, 2007
    Lipofectamine™ 2000 Reagent is a lipid-derived reagent designed to deliver exogenic nucleic acids (DNA and/or RNA) into eukaryotic cells, either adherent or in suspension. Thee two most important ... read more
  • Lipofectamine™ 2000 From Invitrogen

    Lipofectamine™ 2000 From Invitrogen

    Life Technologies
    Tuesday, September 25, 2007
    A common method used in almost every lab these days is transient transfection of cell lines followed by some form of output (Western blot, reporter gene assays, etc.). In a majority of cases, one ... read more
  • PLUS™ Reagent From Invitrogen

    PLUS™ Reagent From Invitrogen

    Life Technologies
    Tuesday, September 25, 2007
    Efficient and effective transfection reagents are essential for protein expression in eukaryotic cells. Recent years have seen a rapid expansion in the number and type of transfection reagents ... read more
  • Lipofectamine™ LTX Transfection Reagent From Invitrogen

    Lipofectamine™ LTX Transfection Reagent From Invitrogen

    Life Technologies
    Tuesday, September 25, 2007
    Invitrogen have added a new product, Lipofectamine™ LTX, to their extensive range of transfection products. Following on from other products in the Lipofectamine range, Lipofectamine™ LTX is a lipid ... read more
  • GeneJuice® Transfection Reagent From Novagen

    GeneJuice® Transfection Reagent From Novagen

    Novagen
    Thursday, February 21, 2008
    Transfection and expression of altered genes into cell lines are indispensable parts of genetic studies. There are many different methods for introducing genetic material into eukaryotic cells, ... read more
  • PolyFect Transfection Reagent From Qiagen

    PolyFect Transfection Reagent From Qiagen

    Thursday, February 28, 2008
    In our experiment, I used Qiagen’s QIAEXII Gel Extraction Kit (Cat. No. 20021) to prepare the DNA prior to transfection so that it was clear of bacterial endotoxins. I used the “desalting protocol” ... read more
  • PolyJet DNA In Vitro Transfection Reagent from SignaGen Laboratories

    PolyJet DNA In Vitro Transfection Reagent from SignaGen Laboratories

    Friday, July 27, 2012
    PolyJet works very well, especially in HEK293 series cells, such as HEK293, HEK293T, HEK293FT. Compared to other transfection reagents, it is much less expensive but shows same efficiency of ... read more
  • PEI - An Inexpensive Transfection Reagent

    PEI - An Inexpensive Transfection Reagent

    Tuesday, March 25, 2014
    My research involves making lentiviral vectors pseudotyped with vesicular stomatitis virus envelop glycoprotein (VSV-G). In this protocol, I transfect HEK-293T cells with 3 plasmids; one for ... read more
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