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  • Strong Signal: Anti-BMI1 IF

    Strong Signal: Anti-BMI1 Immunofluorescence

    EMD Millipore
    Monday, April 07, 2014
    The purpose of this experiment was to identify an antibody that was suitable for detection of BMI1 in human cells by indirect immunofluorescence. read more
  • Strong Signal: Anti-Acetyl-Lysine Immunofluorescence

    Strong Signal: Anti-Acetyl-Lysine Immunofluorescence

    EMD Millipore
    Friday, March 28, 2014
    The purpose of this experiment was to identify an antibody that could detect all acetyl-Lysine by immunofluorescence. Lysine acetylation is a post-translational modification of histone tails and is ... read more
  • Antibody for Ubiquitination Analysis

    Antibody for Ubiquitination Analysis

    EMD Millipore
    Thursday, March 20, 2014
    Ubiquitination is the most important way to regulate proteins in cells. Our lab has demonstrated that FbxL5 can direct SNA1 to degradation thought its polyubiquitination. We use this commercial ... read more
  • Immunocytochemistry for GluR1 Subunit of AMPA Receptors in Hippocampal Neurons

    GluR1 Subunit of AMPA Receptors in Hippocampal Neurons

    EMD Millipore
    Monday, December 30, 2013
    Stress in vivo, and CRH application in vitro, cause dendritic spine loss in hippocampal pyramidal neurons. This loss of spines, and subsequent synapses, is likely the underlying mechanism through ... read more
  • Immunocytochemistry of Surface GluR1 Subunit of AMPA receptors

    GluR1 Subunit of AMPA Receptors

    EMD Millipore
    Wednesday, December 25, 2013
    Stress in vivo, and CRH application in vitro, cause dendritic spine loss in hippocampal pyramidal neurons. This loss of spines, and subsequent synapses, is likely the underlying mechanism through ... read more
  • Kaiso Localization in Normal Mouse Colon

    Kaiso Localization in Normal Mouse Colon

    EMD Millipore
    Tuesday, December 24, 2013
    Kaiso binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote ... read more
  • Glut-4 Detection by IHC-Paraffin

    Glut-4 Detection by IHC-Paraffin

    EMD Millipore
    Friday, December 20, 2013
    GLUT4 is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. We use it as a... read more
  • Great NeuN Antibody

    Great NeuN Antibody

    EMD Millipore
    Thursday, December 19, 2013
    I needed to identify neuronal cell types in mouse brain for stereological analysis. NeuN is a mature neuronal marker that is localized in the nucleus which allows for ease of cell counting. read more
  • Great Olig2 Antibody

    Great Olig2 Antibody

    EMD Millipore
    Wednesday, December 18, 2013
    I needed to identify oligodendroglial cells in mouse brain for stereological analysis. Olig2 is a marker for oligodendroglial cells at all stages of development from immature oligodendrocyte ... read more
  • Hippocampal Cell Lysate WB for RNA Pol II

    Hippocampal Cell Lysate WB

    EMD Millipore
    Tuesday, December 17, 2013
    Stress in vivo, and CRH application in vitro, cause dendritic spine loss in hippocampal pyramidal neurons. This loss of spines, and subsequent synapses, is likely the underlying mechanism through ... read more
  • Staining of Rat Placenta with SATB2

    Staining of Rat Placenta with SATB2

    EMD Millipore
    Wednesday, December 04, 2013
    E9.5 rat placenta was stained with SATB2 at 1/1000 together with nuclear marker DAPI. The tissue was PFA fixed, blocked in 3% BSA and then incubated with the antibody for overnight at 4 degree. An ... read more
  • Staining of Colon Cancer Cell with BRCA1

    Staining of Colon Cancer Cell with BRCA1

    EMD Millipore
    Friday, November 29, 2013
    Human colon cancer cell line was stained with BRCA1 at 1/200 together with nuclear marker DAPI. The cells were PFA fixed, blocked in 3% normal goat serum and then incubated with the antibody for 12 ... read more
  • Oct4 Alexa-flour 488 Conjugated Antibody

    Oct4 Alexa-flour 488 Conjugated Antibody

    EMD Millipore
    Tuesday, October 08, 2013
    I used an human embryonic cell line, H9, to test this conjugated antibody. read more
  • CD3 Antibody for the Violet Laser

    CD3 antibody for the violet laser

    Monday, October 07, 2013
    Multi-color flow cytometry can be tricky, especially when you're doing more than 6 colors. In order to free up some of the more commonly used channels, it's a good idea to use this antibody to stain ... read more
  • APC Anti-Human NKG2D Antibody: Highly Recommended

    APC Anti-Human NKG2D Antibody: Highly Recommended

    BioLegend
    Monday, September 09, 2013
    This antibody was used in a study that investigated the role of NKG2D receptor in the regulation of NK cell migration. This work has been published in European Journal of Immunology in 2012 (Serrano-... read more
  • PE anti-mouse Tim-1

    PE anti-mouse Tim-1

    BioLegend
    Wednesday, August 28, 2013
    Our lab studies murine T cells; specificalloy we are interested in isolating the Tim-1+ subpopulation. Because there is no commercially available kit for for TIM-1+ T cell isolation, we combine the PE... read more
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