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  • Good chemiluminescent substrate for Western blot

    Good chemiluminescent substrate for Western blot

    Thermo Scientific Pierce Protein Research Products
    Friday, May 15, 2015
    I use this chemiluminescent substrate to detect HRP conjugated secondary antibodies in order to detect proteins via Western blot. I used this chemiluminescent substrate because it is an easy two ... read more
  • NE-PER Nuclear and cytoplasmic extraction reagent

    NE-PER Nuclear and cytoplasmic extraction reagent

    Thermo Scientific Pierce Protein Research Products
    Friday, April 10, 2015
    This was used to isolate the nuclear extract from the cell lines in order to check the nuclear protein activity in compound treated cells. It was chosen for its ease of isolation and because it is ... read more
  • Super signal west dura extended duration substrate for developing Western blots

    Super signal west dura extended duration substrate

    Thermo Scientific Pierce Protein Research Products
    Thursday, April 09, 2015
    This product was used to check the differential protein expression in proliferation and differentiation of neuronal cells involved in neuronal protection. This SuperSignal West Dura Extended Duration ... read more
  • Easy to use for protein quantification

    Easy to use for protein quantification

    Thermo Scientific Pierce Protein Research Products
    Wednesday, April 08, 2015
    This product was used in Western blot assays looking at various proteins in the brain homogenate of MPTP-intoxicated animals. After isolating the region of interest, protein was purified, run on a gel... read more
  • Semi-dry Western blot

    Semi-dry Western blot

    Thermo Scientific Pierce Protein Research Products
    Monday, February 23, 2015
    This product is used to transfer proteins from sds-page gel to PVDF membranes of 45kda and ~14 kda proteins. Compared to wet transfer it reduces the time (15min 20v), consumes less transfer buffer (... read more
  • SuperBlock Really is Super

    SuperBlock Really is Super

    Thermo Scientific Pierce Protein Research Products
    Wednesday, November 05, 2014
    I have been working with SuperBlock as a blocking buffer for the last three years and it has dramatically improved the quality of my western blots and ELISA's. The product is very fast and easy to ... read more
  • ImmunoPure® Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit from Thermo Scientific Pierce Protein Research Products

    ImmunoPure® Ultra-Sensitive ABC Peroxidase Rabbit IgG Staining Kit from Thermo Pierce

    Thermo Scientific Pierce Protein Research Products
    Monday, September 17, 2012
    The ImmunoPure® Ultra-Sensitive ABC Peroxidase Staining Kit performed well with sections, Westerns and ELISAs, but detection of immunolocalization on serial paraffin-embedded section is my technique ... read more
  • Luciferase Assay System by Promega

    Luciferase Assay System by Promega

    Promega
    Tuesday, January 31, 2012
    The Luciferase Assay System from Promega is available in two different formats. You can buy the system without Reporter Lysis Buffer or with Reporter Lysis Buffer. Whether you get the Reporter Lysis... read more
  • Mouse IL-1beta ELISA MAX Standard by BioLegend

    Mouse IL-1beta ELISA MAX Standard by BioLegend

    BioLegend
    Thursday, January 26, 2012
    Mouse IL-1beta ELISA MAX™ Standard Kit by BioLegend comes with many of the supplies necessary to complete ELISAs using 5, 10, or 20 96-well plates. The plates are not included in the kit; the kit ... read more
  • Subcellular Protein Fractionation Kit From Thermo Scientific Pierce Protein Research Products

    Subcellular Protein Fractionation Kit From Thermo Scientific Pierce Protein Research Products

    Thermo Scientific Pierce Protein Research Products
    Tuesday, July 06, 2010
    Whole cell protein analysis is an excellent way to assess protein content of a specific cell population. Nevertheless, the shift of proteins into different compartments due to activation, ... read more
  • Pierce ECL Western Blotting Substrate from Thermo Scientific

    Pierce ECL Western Blotting Substrate from Thermo Scientific

    Thermo Scientific Pierce Protein Research Products
    Tuesday, April 27, 2010
    Western Blotting, also called protein immunoblotting, is a very commonly used technique to detect proteins in tissue or cell lysates. Briefly, in this technique, the cell homogenate is separated into ... read more
  • Pyruvate Assay Kit from Biovision Inc.

    Pyruvate Assay Kit from Biovision Inc.

    BioVision
    Wednesday, April 07, 2010
    The carboxylate ion of pyruvic acid, pyruvate, is a central molecule in various intermediary metabolic pathways. Pyruvate is produced from glucose through glycolysis, supplies energy to cells via ... read more
  • Pierce® Cell Surface Protein Isolation Kit From Thermo Scientific

    Pierce® Cell Surface Protein Isolation Kit From Thermo Scientific

    Thermo Scientific Pierce Protein Research Products
    Monday, February 01, 2010
    Cell surface proteins represent a key subset of the cell's total protein, most notably because of the high concentration of integral membrane proteins. These proteins play... read more
  • BCA Protein Assay Kit (bicinchoninic acid) From Thermo Scientific Pierce Protein Research Products

    BCA Protein Assay Kit (bicinchoninic acid) From Thermo Scientific Pierce Protein Research Products

    Thermo Scientific Pierce Protein Research Products
    Monday, January 11, 2010
    Determining the protein concentration of samples is a crucial requirement for most research studies. Since it is an application widely used in all laboratories, several kits and products are... read more
  • ADP Hunter Plus Kit From DiscoveRx

    ADP Hunter Plus Kit From DiscoveRx

    DiscoveRx
    Monday, December 14, 2009
    Kinase assays have been one of the most important tools in the study of biology due to the enormous number of kinases which are directly linked with a variety of disease states. The discovery of ... read more
  • B-PER 6xHis Fusion Protein Purification Kit From Thermo Scientific Pierce Protein Research Products

    B-PER 6xHis Fusion Protein Purification Kit From Thermo Scientific Pierce Protein Research Products

    Thermo Scientific Pierce Protein Research Products
    Wednesday, October 07, 2009
    Production of recombinant protein using bacterial expression systems is a powerful tool available to modern researchers. The ability to utilize E.coli to produce large quantities of a desired protein... read more
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