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  • PTEN Antibody in Western Blot

    PTEN Antibody in Western Blot

    Cell Signaling Technology
    Wednesday, May 20, 2015
    The main purpose of our work is to identify new functional roles of candidate genes in the cellular network. In order to understand their functional roles, we downregulate the candidate genes in ... read more
  • Fantastic p-p65 antibody

    Fantastic p-p65 antibody

    Cell Signaling Technology
    Friday, May 15, 2015
    We study NF-kB activation by monitoring p-p65 over time in bone marrow macrophages upon LPS stimulation by Western Blot. This antibody has been consistently reliable from Day 1, and detects in milk ... read more
  • Western Blot superstar

    Western Blot superstar

    Cell Signaling Technology
    Friday, May 15, 2015
    We grow murine bone marrow derived macrophages and treat them with lipopolysaccharide to determine response of NFkB signaling with a time course. read more
  • Good phospho STAT6 Antibody

    Good phospho STAT6 Antibody

    Cell Signaling Technology
    Tuesday, April 21, 2015
    I was using the phospho-STAT6 antibody to determine if the IL-13 I purchased was causing activation of the JAK/STAT pathway in primary epithelial cells. This is a good antibody to detect the Tyr641 ... read more
  • EPAC1 Western blot

    EPAC1 Western blot

    Cell Signaling Technology
    Tuesday, April 14, 2015
    Western blot of extracts of whole mouse brain was done using EPAC1 antibody. We wanted to see whether the protein was expressed in NHBE cells but I was only able to do a control which is the band in ... read more
  • Very good HA antibody

    Very good HA antibody

    Cell Signaling Technology
    Thursday, April 09, 2015
    I used this anti-HA antibody to stain my N-terminal HA tagged NICD3 protein. NICD3 should primarily be in the nucleus and I see nice nuclear staining with the HA antibody. Overall, this is a good ... read more
  • Hi Fidelity Polymerase

    Hi Fidelity Polymerase

    New England Biolabs
    Thursday, April 09, 2015
    I used this polymerase while performing PCR to subclone a gene fragment into a viral vector so I could transduce primary epithelial cells. Upon sequencing, this polymerase gave a PCR product of the ... read more
  • HER2 Antibody for Immunofluorescence/Western blot

    HER2 Antibody for Immunofluorescence/Western blot

    Cell Signaling Technology
    Monday, April 06, 2015
    Cell Signaling HER2 antibody #2242 was used for both Western blot and immunofluorescence microscopy to determine the level of HER2 silencing by siRNA. This antibody was previously used to detect HER2 ... read more
  • Expression of Phospho-S6 Ribosomal Protein decreased in acute Beta-adrenergic receptor antagonists treated mice

    Expression of Phospho-S6 Ribosomal Protein decreased

    Cell Signaling Technology
    Friday, April 03, 2015
    This antibody was used in a study that investigated the role of Phospho-S6 protein in acute beta-adrenergic receptors antagonist treatment C57B6 mice. The aim of this project is to investigate the ... read more
  • Nuclear and cytoplasmic separation of fibroblasts

    Nuclear and cytoplasmic separation of fibroblasts

    Cell Signaling Technology
    Friday, April 03, 2015
    Nuclear and cytoplasmic proteins from human dermal fibroblasts were separated using salt buffers and centrifugation methods. Separated nuclear and cytoplasmic protein (5ugs) was loaded onto a western ... read more
  • Cell Signaling's VEGFR2 Antibody for Immunofluorescence

    Cell Signaling's VEGFR2 Antibody for Immunofluorescence

    Cell Signaling Technology
    Thursday, April 02, 2015
    This antibody was used in a study that used VEGFR2 as a marker for endothelial cell (EC) differentiation from MSCs. Several antibodies and concentrations were tried, but ultimately Cell Signaling's #... read more
  • Not useful for looking at brain homogenate

    Not useful for looking at brain homogenate

    Cell Signaling Technology
    Thursday, March 26, 2015
    The phosphorylation of TH at the different serine residues was evaluated in MPTP-intoxicated mice. Following MPTP, brain regions were isolated, homogenized, purified, and run on Western blots. ... read more
  • Works well for brain homogenate (mouse)

    Works well for brain homogenate (mouse)

    Cell Signaling Technology
    Wednesday, March 25, 2015
    This antibody was used to look at changes in tyrosine hydroxylase phosphorylation at serine 31 in mice following MPTP. 7 days post-MPTP brain regions of interest were isolated, homogenized, and ... read more
  • Good NICD1 Antibody

    Good NICD1 Antibody

    Cell Signaling Technology
    Thursday, March 12, 2015
    For this experiment, I was trying to determine if the gamma-secretase inhibitor DAPT, at a concentration of 10 micromolar, was inhibiting the formation of NICD1 in primary human bronchial epithelial ... read more
  • Autophagy Western Blot Antibodies

    Autophagy Western Blot Antibodies

    Cell Signaling Technology
    Wednesday, March 11, 2015
    To elucidate the effect of mechanical ventilation on oxidative stress and autophagy proteins on diaphragm of patients. read more
  • Anti-PIAS1 (D33A7) XP

    Anti-PIAS1 (D33A7) XP

    Cell Signaling Technology
    Friday, March 06, 2015
    I have tried several antibodies to detect PIAS1 (endogenous or in condition of over-expression) in cells. So far, this antibody is the best one for both western blot and immunofluorescence analysis. read more
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