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  • Very good flow cytometry machine

    Very good flow cytometry machine

    Monday, May 18, 2015
    This equipment was used for detecting cell surface and intracellular expression of proteins from mouse splenocytes. read more
  • Very Good Anti-Human CD8 antibody

    Very Good Anti-Human CD8 antibody

    Thursday, May 14, 2015
    Human PBMCs were isolated from healthy individuals and the cells were stained with anti human CD4 and CD8 antibody. The result showed a clear CD4 and CD8 positive populations, which confirms the ... read more
  • Good and Useful CD25 Antibody

    Good and Useful CD25 Antibody

    Wednesday, May 13, 2015
    We selected CD25 Antibody from Biolegend specifically because CD25 is expressed in activated cells and can be found in lymph nodes. Using this product I was able to differentiate activated cell ... read more
  • Very Good Anti-Human CD4 antibody

    Very Good Anti-Human CD4 antibody

    Tuesday, May 12, 2015
    Human PBMC reconstituted NOD-scid IL2r?cnull mice (NSG mice), were sacrificed and single cell suspensions from the spleen were used for flow cytometry. This antibody was used for detecting human CD4 ... read more
  • Good antibody for HIF-1 alpha

    Good antibody for HIF-1 alpha

    Tuesday, May 05, 2015
    This antibody is used as a control to make sure CoCl2, a hypoxia mimetic, is increasing HIF protein levels in HeLa cells. read more
  • Excellent Human CD45 Antibody

    Excellent Human CD45 Antibody

    Friday, May 01, 2015
    Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. I have used this antibody to separate human cells from the spleen of these ... read more
  • Very Good Anti-Human HLA-DR  antibody

    Very Good Anti-Human HLA-DR antibody

    Wednesday, April 29, 2015
    Human PBMC reconstituted NOD-scid IL2r?cnull mice (NSG mice), were sacrificed and single cell suspensions from spleen was used for flow cytometry. This antibody was used for detecting human HLA-DR ... read more
  • Very Good Anti-Human PD-1  antibody

    Very Good Anti-Human PD-1 antibody

    Tuesday, April 28, 2015
    Human PBMC reconstituted NOD-scid IL2r?cnull mice (NSG mice), were sacrificed and single cell suspensions from spleen was used for flow cytometry. This antibody was used for detecting PD-1 expression ... read more
  • Very Good Anti-Human CD8 antibody

    Very Good Anti-Human CD8 antibody

    Monday, April 27, 2015
    NOD-scid IL2rgammac-null mice (NSG mice) reconstitute with human PBMCs were sacrificed and single cell suspensions prepared from spleen was used for flow cytometric analysis. The cells were stained ... read more
  • Very Good Anti-Human CD45  antibody

    Very Good Anti-Human CD45 antibody

    Thursday, April 23, 2015
    Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. I have used this antibody to separate human cells from the spleen of these ... read more
  • Immunoprecipitaiton (IP) Using ITCH Antibody

    Immunoprecipitaiton (IP) Using ITCH Antibody

    Monday, March 30, 2015
    Immunoprecipitation of ITCH from KB cell extracts using ITCH (BD Biosciences: Cat# 611199) mouse mAb (lane 1) or mouse IgG isotype Control (lane 2) was performed. Western blot analysis was performed ... read more
  • Excellent FACS machine for multiple color staining

    Excellent FACS machine for multiple color staining

    Thursday, March 19, 2015
    We routinely use the BD LSR II for multiple color staining, generally around 10 colors at the same time. read more
  • Th1/Th2/Th17 CBA for cytokine production analysis

    Th1/Th2/Th17 CBA for cytokine production analysis

    Wednesday, March 04, 2015
    The aim of my research is to determine the immune modulating capabilities of drug treatments in Parkinson's disease mouse models. I look at the ability of drug therapies to modulate the T cell ... read more
  • The use of T75 flasks in culture of BV-2 cells

    The use of T75 flasks in culture of BV-2 cells

    Tuesday, March 03, 2015
    The microglial cell line, BV-2, is cultured every 3-4 days in new medium in a T75 flask. These T75 flasks work great for the culturing of BV-2 cells. With the use of the T75 flask I am able to ... read more
  • Use of 18G Precision Glide Needle In Performing Single Cell Suspension

    Use of 18G Precision Glide Needle In Performing Single Cell Suspension

    Friday, February 27, 2015
    After spleens have been removed from mice, a single cell suspension of splenocytes is performed using a 18G Precision Glide Needle. read more
  • BD Pharmigen CD11c HL3 Clone

    BD Pharmigen CD11c HL3 Clone

    Friday, February 06, 2015
    Our experiments assess the tendencies of environmental pollutants to affect dendritic cells' protein expression. We incubated Bone-Marrow Derived DCs with pollutant and collected data via flow ... read more
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