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  • Alpha Amylase Detection in Pancreas

    Alpha Amylase Detection in Pancreas

    Friday, January 24, 2014
    a-Amylase is a protein enzyme EC 3.2.1.1 that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Pancreatic a-amylase randomly ... read more
  • ANTI-FLAG® M2 Affinity Gel from Sigma-Aldrich

    ANTI-FLAG® M2 Affinity Gel from Sigma-Aldrich

    Monday, March 11, 2013
    We had a system with cells stably overexpressing FLAG-tagged RBPs. By transfecting the small RNA duplexes and immunoprecipitating (IP’ing) the FLAG-tagged RBPs, we could then perform a Northern blot ... read more
  • Antibody for HA Tagging a Protein

    Antibody for HA Tagging a Protein

    Friday, March 21, 2014
    Hemagglutinin (HA) epitope tag, or HA tag, is one of the most usual ways to mark and detect a protein in vivo. Good antibody for HA tag is essential to many popular laboratory techniques as WB, IF or ... read more
  • Biohit Proline Electronic Multichannel Pipettor From Sigma

    Biohit Proline Electronic Multichannel Pipettor From Sigma

    Tuesday, January 02, 2007
    The Biohit Proline 710800 is an 8-channel electronic repeat pipettor that is capable of pipetting 50-1200 ul of liquid at a given time. This electronic pipettor is easy to program (all done with six ... read more
  • Cell Growth Determination Kit, MTT Based From Sigma

    Cell Growth Determination Kit, MTT Based From Sigma

    Thursday, June 19, 2008
    The Cell Growth Determination Kit from Sigma is a colorimetric method for determining the number of viable cells in culture and is useful in the measurement of cell growth in response to mitogens, ... read more
  • GenElute Plasmid Prep Kit From Sigma-Aldrich

    GenElute Plasmid Prep Kit From Sigma-Aldrich

    Tuesday, September 29, 2009
    In the modern molecular world almost every biology lab today finds itself working with plasmid DNA in some capacity. One of the simplest tasks required is purifying plasmid DNA from small... read more
  • GenElute™ Gel Extraction Kit From Sigma

    GenElute™ Gel Extraction Kit From Sigma

    Wednesday, September 27, 2006
    I use the GenElute™ Gel DNA Extraction Kit from Sigma when I want to carry out extraction of plasmid DNA from agarose gels. Most of my plasmid DNA extractions vary between 200bp (for an insert ... read more
  • GenElute® PCR Clean-up Kit From Sigma-Aldrich

    GenElute® PCR Clean-up Kit From Sigma-Aldrich

    Friday, February 02, 2007
    The GenElute® PCR Clean-Up Kit is designed for rapid purification of single-stranded or double-stranded PCR amplification products (100 bp to 10 kb) from other components in the reaction, such as ... read more
  • Genomeplex® Whole Genome Amplification (WGA) Kit by Sigma-Aldrich

    Genomeplex® Whole Genome Amplification (WGA) Kit by Sigma-Aldrich

    Wednesday, January 31, 2007
    The GenomePlex® Whole Genome Amplification method uses input DNA to generate a library of amplified DNA fragments that faithfully represents the original input genome. The three step fragmentation, ... read more
  • GFP Antibody for IF

    GFP Antibody for IF

    Monday, December 16, 2013
    Stress in vivo, and CRH application in vitro, cause dendritic spine loss in hippocampal pyramidal neurons. This loss of spines, and subsequent synapses, is likely the underlying mechanism through ... read more
  • Insulin Detection in Pancreas

    Insulin Detection in Pancreas

    Wednesday, December 25, 2013
    Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. Insulin causes cells in the liver, skeletal muscles, and... read more
  • Loading Control for WB: Tubulin

    Loading Control for WB: Tubulin

    Monday, January 27, 2014
    Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins and a sixth ... read more
  • Molecular Weight Market Kit for Gel Filtration from Sigma-Aldrich

    Molecular Weight Market Kit for Gel Filtration from Sigma-Aldrich

    Wednesday, March 24, 2010
    Molecular weight markers for gel filtration are essential components for standardization of the column and for size prediction of an unknown sample. In gel filtration, the samples are run through a ... read more
  • Monoclonal Anti-Desmin Antibody from Sigma-Aldrich, used in Western Blotting

    Monoclonal Anti-Desmin Antibody from Sigma-Aldrich, used in Western Blotting

    Tuesday, February 14, 2012
    In vivo, we analyzed regenerating skeletal muscle, otherwise desmin expression was very low; in vitro desmin is normally expressed by myogenic cell lines / primary cultures. Our results were quite ... read more
  • Native Protein Deglycosylation Kit from Sigma-Aldrich

    Deglycosylation Kit from Sigma-Aldrich

    Tuesday, February 19, 2013
    The Native Protein Deglycosylation Kit is used to remove N-linked oligosaccharides from glycoproteins under native conditions as opposed to more traditional methods. Glycosylation protein ... read more
  • Pancreas Metabolism by IHC-Paraffin

    Pancreas Metabolism by IHC-Paraffin

    Tuesday, December 24, 2013
    The main functions of pancreatic glucagon consist of gluconeogenesis, ketogenesis, lipolysis and glycogenolysis. Monoclonal anti-Glucagon antibody can be used as an analytical tool for ... read more
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