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  • Taq PCR Core Kit From Qiagen

    Taq PCR Core Kit From Qiagen

    Monday, January 15, 2007
    As part of my job, I routinely genotype mice for various labs which breed multiple strains of mice for use in functional studies of disease processes. Of all the kits and Taq polymerases that I have ... read more
  • TransMessenger Transfection Reagent with RNA and si-RNA From Qiagen

    TransMessenger Transfection Reagent with RNA and si-RNA From Qiagen

    Thursday, February 01, 2007
    TransMessenger Transfection Reagent is a lipid formulation-based transfection method for getting small-interfering RNAs (siRNAs) into cells in culture. The Transmessenger system is used with an RNA ... read more
  • QIAprep Spin Miniprep Kit From Qiagen

    QIAprep Spin Miniprep Kit From Qiagen

    Friday, February 02, 2007
    The QIAprep Miniprep system is intended for isolation of plasmids from bacteria cells. The kit uses silica-gel-membrane technology to bind DNA, from which the DNA can be eluted with an elution buffer... read more
  • Isolating DNA: A Comparison Between DNeasy Blood & Tissue Kit From Qiagen, PureGene Tissue Kit From Gentra and Wizard® Genomic DNA Purification Kit From Promega

    Isolating DNA: A Comparison Between DNeasy Blood & Tissue Kit From Qiagen, PureGene Tissue Kit From Gentra and Wizard® Genomic DNA Purification Kit From Promega

    Wednesday, February 21, 2007
    Isolating DNA is usually easy and straightforward. However, there are sample sources that can be tricky. Some insects, for example, have a reputation of being notoriously hard to isolate DNA from, or ... read more
  • Large-Construct Kit From Qiagen

    Large-Construct Kit From Qiagen

    Friday, March 09, 2007
    The purification of large DNA constructs from bacteria such as BACs, PACs, P1s, and cosmids can be troublesome. Not only are these constructs usually present at low copy number... read more
  • QIAquick Gel Extraction (and PCR Purification) Kit From Qiagen

    QIAquick Gel Extraction (and PCR Purification) Kit From Qiagen

    Wednesday, March 14, 2007
    Qiagen’s QIAquick Gel Extraction Kit is highly effective for extracting high purity, double-stranded DNA from either PCR reactions or agarose gels following gel electrophoresis. In either case, the ... read more
  • QIAquick Gel Extraction Kit From Qiagen

    QIAquick Gel Extraction Kit From Qiagen

    Wednesday, March 14, 2007
    The QIAquick Gel Extraction Kit is intended for extraction of DNA fragments ranging in size from 70 bp to10 kb from agarose gels in TAE or TBE buffer. The kit uses silica-gel-membrane technology to ... read more
  • QIAquick PCR Purification Kit From Qiagen

    QIAquick PCR Purification Kit From Qiagen

    Wednesday, March 14, 2007
    One of the most popular kits in a molecular biology lab, besides DNA miniprep kit, is a PCR purification kit. In our lab, we extensively use Qiagen’s QIAquick PCR Purification Kit which utilizes spin ... read more
  • MinElute Gel Extraction Kit From Qiagen

    MinElute Gel Extraction Kit From Qiagen

    Wednesday, March 14, 2007
    Qiagen’s MinElute Gel extraction Kit is very similar to their QIAquick Gel Extraction kit, however, it uses a slightly different column that allows you to elute the DNA in a much smaller volume (10 ul... read more
  • Plasmid Maxi Kit From Qiagen

    Plasmid Maxi Kit From Qiagen

    Wednesday, March 14, 2007
    We use Qiagen’s Plasmid Maxi kits to isolate large quantities (500-800 ug) of DNA suitable for sequencing, restriction digests and even for transfection of mammalian cells. We continue to use these ... read more
  • MinElute PCR Purification Kit From Qiagen

    MinElute PCR Purification Kit From Qiagen

    Friday, March 16, 2007
    For some downstream assays, such as ligation and transformation, restriction enzyme digestion, in vitro transcription, hybridization and radioactive and fluorescent sequencing, a researcher has to ... read more
  • Ni-NTA Agarose From Qiagen

    Ni-NTA Agarose From Qiagen

    Tuesday, March 27, 2007
    The Nickel Nitrilo-triacetic Acid (NTA) is a Qiagen-patented resin which offers affinity purification of 6Xhis-tagged proteins expressed as recombinant proteins from sources like E. coli, insect (... read more
  • QIAquick® Gel Extraction Kit From Qiagen

    QIAquick® Gel Extraction Kit From Qiagen

    Friday, March 30, 2007
    Extraction of pure DNA from agarose is essential for many downstream assays including cloning and radioisotopic or fluorescent DNA sequencing. We use Qiagen’s QIAquick Gel Extraction Kit for this ... read more
  • Qiaquick® Gel Extraction Kit from Qiagen

    Qiaquick® Gel Extraction Kit from Qiagen

    Thursday, April 26, 2007
    This whole procedure works via spin columns, which is highly comfortable and efficient. Isolation and extraction of gel fragments from agarose gels has come a long way. Specifically, in laboratories ... read more
  • OneStep RT-PCR Kit From Qiagen

    OneStep RT-PCR Kit From Qiagen

    Bio-Rad
    Wednesday, May 16, 2007
    The QIAGEN OneStep RT-PCR Kit allows reverse transcription and PCR amplification to be performed sequentially in a single tube, referred to as a “one-step” reaction. The QIAGEN OneStep RT-PCR Enzyme ... read more
  • DNeasy Blood And Tissue Kit From Qiagen

    DNeasy Blood And Tissue Kit From Qiagen

    Friday, May 25, 2007
    DNeasy® Blood and Tissue Kits provide all reagents and most of the tubes needed for rapid purification of total genomic DNA from a variety of different tissues, cells, blood and bacteria. The kit is ... read more
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