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  • Good antibody for ChIP assay of MeCP2 binding DNA sequence

    Good antibody for ChIP assay of MeCP2 binding DNA sequence

    Cell Signaling Technology
    Wednesday, February 25, 2015
    My focus of work is studying the critical role of MeCP2 (methyl-CpG binding protein 2), whose mutation causes Rett Syndrome, in the development and function of the nervous system. I discovered a novel... read more
  • Great MeCP2 antibody for immunohistochemistry

    Great MeCP2 antibody for immunohistochemistry

    Cell Signaling Technology
    Monday, February 23, 2015
    My focus of work is studying the critical role of MeCP2 (methyl-CpG binding protein 2), which causes Rett Syndrome if mutated, in the development and function of the nervous system. I discovered a ... read more
  • Phospho vimentin S83

    Phospho vimentin S83

    Cell Signaling Technology
    Friday, February 20, 2015
    Vimentin is a cytoskeletal protein that is increasingly gaining importance in the field of cancer research. It is important to know the post-translational status of vimentin that will provide us novel... read more
  • Phospho vimentin S56

    Phospho vimentin S56

    Cell Signaling Technology
    Friday, February 20, 2015
    Vimentin is a cytoskeletal protein that is increasingly gaining importance in the field of cancer research. It is important to know the post-translational status of vimentin that will provide us novel... read more
  • MeCP2 antibody for immunostaining of culture cells

    MeCP2 antibody for immunostaining of culture cells

    Cell Signaling Technology
    Thursday, February 19, 2015
    The long-term goal of our lab is to understand the molecular and genetic mechanisms underlying brain development and diseases. My focus is studying the critical role of MeCP2 (methyl-CpG binding ... read more
  • CD4+CD25+ Reg T cell isolation kit, mouse

    CD4+CD25+ Reg T cell isolation kit, mouse

    Miltenyi Biotec
    Friday, February 13, 2015
    The general aim of my research was to test the ability of Regulatory T cells from drug treated mice to suppress the proliferation of effector T cells. I used this product in order to isolate the CD4+... read more
  • Great immunoblotting antibody

    Great immunoblotting antibody

    Cell Signaling Technology
    Thursday, February 12, 2015
    I wanted to quantify GluR4 protein expression in brain tissue homogenates. I initially tried the rabbit polyclonal anti-GluR4 from the same company. When I showed that it did not work, I was sent a ... read more
  • A good antibody for detecting Ubc9

    A good antibody for detecting Ubc9

    Cell Signaling Technology
    Thursday, February 05, 2015
    Protein SUMOylation is an important post-translational modification required for normal physical functions of a cell. Ubc9 is an evolutionarily conserved SUMO E2 enzyme involving in the SUMO ... read more
  • Great loading control antibody

    Great loading control antibody

    Cell Signaling Technology
    Tuesday, February 03, 2015
    I wanted to investigate whether the target proteins had any diurnal variations in olfactory bulb tissue and investigated whether these proteins varied between biological replicates and over the course... read more
  • Good Antibody, Needs a lot to detect Western bands

    Good Antibody, Needs a lot to detect western bands

    Cell Signaling Technology
    Tuesday, January 20, 2015
    This product is one of few phospho antibodies on the market for Pin1. It comes as 50ul but about a 1:100 to 1:500 dilution is necessary to detect phosphorylation reliably. The blot is often dirty, ... read more
  • Nice LC3B antibody for tougher protein

    Nice LC3B antibody for tougher protein

    Cell Signaling Technology
    Tuesday, January 20, 2015
    This antibody was used in a study that investigated the role of LC3-B in autophagy. Several LC3-B antibodies were tested in this study for various applications; the antibody reviewed here (2775) gave ... read more
  • Works for chemiluminescence but not well for fluorescence - Unattributed Review*

    Works for chemiluminescence but not well for fluorescence - Unattributed Review*

    Cell Signaling Technology
    Monday, January 19, 2015
    I wanted to measure GluR2 protein levels in brain tissue homogenates via immunoblot. The bands visible in the blot were not crisp and clear, but still at the appropriate weight. No side bands were ... read more
  • Turtle CREB Antibody for chromatin immunoprecipitation

    Turtle CREB Antibody for chromatin immunoprecipitation

    Cell Signaling Technology
    Thursday, January 08, 2015
    We are investigating the regulatory mechanisms controlling BDNF expression in an in vitro model of eyeblink classical conditioning, we analyzed the transcription factors and other signaling proteins ... read more
  • LC3A/B human antibody, one of the best autophagy markers!

    LC3A/B human antibody, one of the best autophagy markers!

    Cell Signaling Technology
    Thursday, January 08, 2015
    The CST LC3A/B antibody is one of the most widely used autophagy markers on the market. It is usable in immunoblot assays in which the detection of the slightly lower band (also called LC3II) ... read more
  • IF of Chk1 pS345

    IF of Chk1 pS345

    Cell Signaling Technology
    Monday, January 05, 2015
    Chk1 is phosphorylated on Ser 317 and Ser 345 by ATR protein kinases after DNA damage. Phosphorylation of Chk1 at S317 and S345 is a hallmark of activation of the cell cycle checkpoint. The UV-induced... read more
  • Western blot of Phospho Chk1 pS317

    Western blot of Phospho Chk1 pS317

    Cell Signaling Technology
    Monday, December 29, 2014
    Chk1 is an effector kinase that acts downstream of ATR in response to DNA damage and replication stress. Phosphorylation of Chk1 at S317 and 345 are hallmarks of the activation cell cycle checkpoint. ... read more
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