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  • Ni-NTA Agarose From Qiagen

    Ni-NTA Agarose From Qiagen

    Thursday, September 06, 2007
    The Nickel-Nitrilo triacetic acid (Ni-NTA) coupled to Sepharose CL-6B from Qiagen offers a quick and efficient method of protein purification from small and large scale preparations obtained through ... read more
  • PrimeSTAR™ HS DNA Polymerase

    PrimeSTAR™ HS DNA Polymerase

    Takara Bio
    Wednesday, May 24, 2006
    The reduced mutation frequency of PrimeStar™ is attributed to the enzyme’s strong exonuclease activity, resulting in 12 errors/250,000 bp. Takara also claims that PrimeStar™ has a higher efficiency ... read more
  • DNA Ligation Kit, Version 1 From Takara

    DNA Ligation Kit, Version 1 From Takara

    Takara Bio
    Monday, January 08, 2007
    How did I get to know the DNA Ligation Kit from Takara? Around 2 years ago, I was trying to do a cloning and I was stuck at the ligation step. At first, I was using the regular T4 DNA ligase and ... read more
  • SYBR® Premix Ex Taq™ (Perfect Real Time) From Takara Mirus Bio

    SYBR® Premix Ex Taq™ (Perfect Real Time) From Takara Mirus Bio

    Takara Bio
    Monday, January 08, 2007
    SYBR® Premix Ex Taq™ (Perfect Real Time) is a 2X concentration of premixed reagent which includes Takara’s Ex Taq™ HS and SYBR® Green I. The Ex Taq™ HS is an enzyme for hot start PCR... read more
  • Chaperone Plasmid Set From Takara Mirus Bio

    Chaperone Plasmid Set From Takara Mirus Bio

    Takara Bio
    Friday, January 26, 2007
    As with many other laboratories, we rely heavily on large-scale recombinant protein expression in our effort to conduct genetic and proteomic analysis of parasite-host cell interactions. There are ... read more
  • LA Taq™ With GC Buffer From Takara

    LA Taq™ With GC Buffer From Takara

    Takara Bio
    Friday, October 05, 2007
    TaKaRa LA (Long and accurate) Taq™ is a thermostable DNA polymerase having 3'-5' exonuclease activity (proof reading activity) that is capable of, under general PCR conditions, higher efficiency and ... read more
  • Single Tube PCR Kit From Takara Bio

    Single Tube PCR Kit From Takara Bio

    Takara Bio
    Wednesday, June 21, 2006
    The Single-Tube PCR Kit from Takara allows nucleic acid extraction and the subsequent PCR to occur in a single tube in a short amount of time. It can be used with small volumes (3 to 10ul) of whole ... read more
  • CD11c Microbeads, Mouse Miltenyi Biotech

    CD11c Microbeads, Mouse Miltenyi Biotech

    Monday, June 11, 2012
    To isolate dendritic cells using the CD11c Microbeads is quite simple. Essentially, one must first obtain of a single cell suspension and to that suspension you will have to apply an appropriate ... read more
  • Mouse T Cell Enrichment Columns from R&D Systems

    Mouse T Cell Enrichment Columns from R&D Systems

    Tuesday, June 26, 2012
    The mouse T cell enrichment kit can be used for the purification of T cells from mouse splenocyte samples. Provided with the kit are the columns as well as the column wash buffer. The kit works via ... read more
  • ANTI-FLAG® M2 Affinity Gel from Sigma-Aldrich

    ANTI-FLAG® M2 Affinity Gel from Sigma-Aldrich

    Monday, March 11, 2013
    We had a system with cells stably overexpressing FLAG-tagged RBPs. By transfecting the small RNA duplexes and immunoprecipitating (IP’ing) the FLAG-tagged RBPs, we could then perform a Northern blot ... read more
  • Recombinanat Protein A-Sepharose 4B from Life Technologies

    Protein A Sepharose for Isolation of Chimeric Proteins

    Life Technologies
    Thursday, April 25, 2013
    Protein A binds the Fc region of immunoglobulins through interaction with the heavy chains. Beads coated with protein A can therefore be used to isolate chimeric recombinant proteins that have been ... read more
  • SapphireAmp Fast PCR Master Mix

    SapphireAmp Fast PCR Master Mix

    Takara Bio
    Thursday, February 06, 2014
    This reagent is designed to rapidly set up a hot start PCR. This made it ideal for large scale genotyping to assess a single nucleotide polymorphism of interest. The reagents were successful in a ... read more
  • PrimeSTAR GXL DNA Polymerase Amplifies Genomic DNA from Crude C. elegans Extracts

    PrimeSTAR GXL DNA Polymerase

    Takara Bio
    Thursday, February 20, 2014
    For this project, my goal was to use a PCR-based strategy to genotype C. elegans strains in order to confirm the presence or absence of specific mutations. Because I was genotyping anywhere from 4-16... read more
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