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  • Good antibody for detecting Lamin A/C

    Good antibody for detecting Lamin A/C

    Santa Cruz Biotechnology, Inc.
    Tuesday, April 14, 2015
    I used this antibody to detect Lamin A/C in cytoplasmic (cyto.) and nuclear (nucl.) fractionated samples to assay the level of contamination in the different fractions. The Lamin A/C shows up in the ... read more
  • MUC5AC Immunfluorescence Staining in NHBE

    MUC5AC Immunfluorescence Staining in NHBE

    Santa Cruz Biotechnology, Inc.
    Monday, April 13, 2015
    This antibody was purchased to use as a marker of goblet cells in airway epithelial cells. read more
  • HILIC for separation of polar metabolites

    HILIC for separation of polar metabolites

    Phenomenex
    Thursday, April 02, 2015
    The HILIC kinetex column has been great for separating out those residues that just wont separate by RP HPLC. The biggest challenge has been getting analytes into a solution suitable for HILIC initial... read more
  • Mouse anti-V-ATPase antibody

    Mouse anti-V-ATPase antibody

    Santa Cruz Biotechnology, Inc.
    Monday, March 30, 2015
    My project involves analysis of the physiological role of the endocytic recycling regulator EHD4 in the kidney. EHD4 is expressed in the inner medullary collecting duct (IMCD) of the renal nephron, ... read more
  • Poor Detection

    Poor Detection

    Santa Cruz Biotechnology, Inc.
    Friday, March 20, 2015
    Have to use at a very strong concentration. Even with probing in 5% milk (blocked in 10% milk for 1 hr at RT), blot came out extremely dirty with lots of adduct bands. I recommend Millipore's ADAM10... read more
  • Detecting cytochrome c by immunofluorescence

    Detecting cytochrome c by immunofluorescence

    Santa Cruz Biotechnology, Inc.
    Wednesday, March 18, 2015
    We are investigating the role of cytochrome c during different apoptosis paradigms. One of the goals is to determine the subcellular localization of cytochrome c during apoptosis. To detect its ... read more
  • Great Housekeeping and Cell Cycle Antibody

    Great Housekeeping and Cell Cycle Antibody

    Santa Cruz Biotechnology, Inc.
    Monday, March 09, 2015
    We used the antibody to determine amounts of CDK in samples used for in vitro kinase assay after pulldown and after Westen blotting of crude extracts. read more
  • Detecting PDI by immunofluorescence

    Detecting PDI by immunofluorescence

    Santa Cruz Biotechnology, Inc.
    Thursday, February 26, 2015
    We are investigating the role of PDI during ER stress-induced apoptosis. One of our goals is to determine the subcellular localization of PDI during apoptosis. To detect its intracellular localization... read more
  • Nocodazole treated cell division inhibition

    Nocodazole treated cell division inhibition

    Santa Cruz Biotechnology, Inc.
    Tuesday, February 24, 2015
    Cell reprogramming using nocodazole is a very old method and works very well with any given type of cells. This is an antimitotic agent that disrupts microtubules by binding to ß-tubulin and also ... read more
  • Protein quantification from brain homogenate

    Protein quantification from brain homogenate

    Santa Cruz Biotechnology, Inc.
    Tuesday, February 24, 2015
    This antibody was used to look at the amount of tyrosine hydroxylase protein in brain homogenates of MPTP-intoxicated mice. Midbrain containing the SN and striatal areas of the brain of mice were ... read more
  • ER-alpha Antibody for Western Blot

    ER-alpha Antibody for Western Blot

    Santa Cruz Biotechnology, Inc.
    Thursday, February 12, 2015
    This is the best antibody against ER-alpha that I have so far used. I used this antibody for detection of ER-alpha during different stages of breast cancer models. I chose the antibody because of its ... read more
  • Perfect antibody for blood typing

    Perfect antibody for blood typing

    Santa Cruz Biotechnology, Inc.
    Friday, December 19, 2014
    Primate blood typing is very important for organ transplantation. We found this product, as a second antibody, works very well for determining baboon blood type when combined with Group-A and group-B ... read more
  • Excellent loading control

    Excellent loading control

    Santa Cruz Biotechnology, Inc.
    Friday, December 12, 2014
    Most of the antibodies are normalized with respect to GAPDH. This antibody is very specific and particularly inexpensive. It works both with human and mouse protein extracts in Immunofluroscence and ... read more
  • Mitochondrial marker for WB- AIF antibody

    Mitochondrial marker for WB- AIF antibody

    Santa Cruz Biotechnology, Inc.
    Wednesday, November 26, 2014
    HEK293 cells were fractionated into cytosol and heavy membrane fractions. AIF was utilized to track the purity of the fractionation as AIF is a mitochondrial protein. read more
  • Anti HSP-10 staining on stem cells

    Anti HSP-10 staining on stem cells

    Santa Cruz Biotechnology, Inc.
    Monday, November 17, 2014
    The antibody was used to detect the effect of environmental toxicants on mitochondrial unfolded protein response. Specifically, I used this product to stain for heat shock protein -10 in stem cells ... read more
  • MCP-1 detection in A549 cells

    MCP-1 detection in A549 cells

    Santa Cruz Biotechnology, Inc.
    Monday, November 10, 2014
    MCP-1 protein expression was characterized via Western blot by using purified MCP-1 (C-17) Antibody read more
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