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  • Excellent Antibody for T-Cell Activation

    Excellent Antibody for T-Cell Activation

    eBioscience
    Tuesday, March 11, 2014
    My research focuses on T cell based immunotherapy. For this, I regularly activate and expand primary T cells from PBMCs. Anti-CD3 antibody is a very potent tool to activate T cells (in conjunction ... read more
  • A Good Anti-Mouse CD3e Antibody

    A Good Anti-Mouse CD3e Antibody

    eBioscience
    Tuesday, October 01, 2013
    We were trying to identify different subsets of T cell memory in mice. The first step in the staining is to gate on CD3+ cells among the lymphocytic gate on mouse splenocytes. We did this using this ... read more
  • Bright and good separation

    Bright and good separation

    eBioscience
    Thursday, September 05, 2013
    We measure Sca-1 in mice. I use this antibody in my multi-color flow-cytometry panel. Anti-mouse Sca-1 eF605NC works great at the recommended concentration for cells from the aorta. The IgG is low ... read more
  • Anti-Baculovirus Envelope gp-64 from eBioscience

    Anti-Baculovirus Envelope Antibody Used in Flow Cytometry

    eBioscience
    Wednesday, May 29, 2013
    This antibody is used for the detection of the baculovirus envelope protein gp-64. GP-64 protein is upregulated on the surface of insect cells shortly after transfection with baculovirus. Insect cells... read more
  • Human IFN-alpha Platinum ELISA Kit from eBioscience

    Human IFN-alpha Platinum ELISA Kit from eBioscience

    eBioscience
    Wednesday, February 08, 2012
    Measuring the concentration of total interferon-alpha secreted by cells in peripheral blood samples, serum samples or even culture supernatants is not an easy task. There are 14 different isoforms of ... read more
  • Mouse IL-6 ELISA Ready-SET-Go! Kit (with Pre-Coated Plates) from eBioscience

    Mouse IL-6 ELISA Ready-SET-Go! Kit (with Pre-Coated Plates) from eBioscience

    eBioscience
    Monday, January 30, 2012
    The eBioscience IL-6 Ready-SET-Go! ELISA kit offers an easy and sensitive method to quantify IL-6 levels in biological samples. This is a ready-to-use kit which contains all of the required chemicals ... read more
  • Rat TNF alpha ELISA Ready-SET-Go! from eBioscience

    Rat TNF alpha ELISA Ready-SET-Go! from eBioscience

    eBioscience
    Monday, January 30, 2012
    The eBioscience TNF alpha Ready-SET-Go!® ELISA kit offers an easy and sensitive method to quantify TNF alpha in biological samples. Using this kit, we have estimated TNF alpha levels in the plasma ... read more
  • Human IL-8 ELISA Ready-SET-Go!  by eBioscience

    Human IL-8 ELISA Ready-SET-Go! by eBioscience

    eBioscience
    Wednesday, November 30, 2011
    The Human IL-8 ELISA, Ready-SET-Go!® Kit by eBioscience comes ready to use; with this kit you can run from 1 to 10 96-well ELISA plates. The plates are not included in the kit, but everything else ... read more
  • QuickLyse Miniprep Kit From Qiagen

    QuickLyse Miniprep Kit From Qiagen

    Thursday, June 19, 2008
    Purification of plasmids from bacteria is critical for a variety of molecular biology applications; however, traditional methods for plasmid purification include multiple steps and can be time-... read more
  • PureLink™ HiPure Plasmid Filter Maxiprep Kit From Invitrogen

    PureLink™ HiPure Plasmid Filter Maxiprep Kit From Invitrogen

    Life Technologies
    Thursday, March 20, 2008
    The PureLink™ HiPure Plasmid Maxiprep Kit from Invitrogen is designed to isolate plasmid DNA of the highest purity from 25 to 500 mls of bacterial culture. The amount of culture is dependent... read more
  • HiSpeed Plasmid Purification Midi Kit From Qiagen

    HiSpeed Plasmid Purification Midi Kit From Qiagen

    Thursday, February 21, 2008
    The HiSpeed Plasmid Purification Kit fits very well into Qiagen’s established line of products for DNA preparation and purification. The buffers used with this kit are identical to the ones used in a ... read more
  • Human or Mouse Regulatory T Cell Staining Kits From eBioscience

    Human or Mouse Regulatory T Cell Staining Kits From eBioscience

    eBioscience
    Thursday, October 25, 2007
    The regulatory T cell staining kits from eBioscience are designed to identify the subpopulation of T cells called T regulatory cells. These cells are characterized by expression of CD4 and high levels... read more
  • HiSpeed Plasmid Maxi Kit From Qiagen

    HiSpeed Plasmid Maxi Kit From Qiagen

    Tuesday, September 25, 2007
    One of the exceptional features of the HiSpeed Plasmid Maxi Kit from Qiagen is that it involves no centrifugation step (except to pellet the bacterial cells). The QIAfilter Cartridges enable rapid and... read more
  • QIAprep Spin Miniprep Kit From Qiagen

    QIAprep Spin Miniprep Kit From Qiagen

    Friday, February 02, 2007
    The QIAprep Miniprep system is intended for isolation of plasmids from bacteria cells. The kit uses silica-gel-membrane technology to bind DNA, from which the DNA can be eluted with an elution buffer... read more
  • PureYield™ Plasmid Midiprep System From Promega

    PureYield™ Plasmid Midiprep System From Promega

    Promega
    Monday, January 08, 2007
    Many protein expression procedures require large amounts of pure plasmid DNA. Over the years, a number of kits have been developed to streamline the isolation of plasmid DNA from large bacterial ... read more
  • ChargeSwitch® Plasmid ER Mini Kit From Invitrogen

    ChargeSwitch® Plasmid ER Mini Kit From Invitrogen

    Life Technologies
    Thursday, October 19, 2006
    There are so many miniprep kits available today that it difficult to choose which one would be the best for your application. I have used several different techniques and a variety of kits for ... read more
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