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  • Intracellular detection of IFN-gamma

    Intracellular detection of IFN-gamma

    BioLegend
    Monday, March 02, 2015
    Mouse transgenic OT-1 cells were stimulated with their cognate peptide: OVA for 3 days. On the last day, cells were re-stimulated with PMA/ionomycin and treated with Brefeldin A to allow accumulation ... read more
  • Galectin-1 Immunofluorescence Detection

    Galectin-1 Immunofluorescence Detection

    R&D Systems
    Monday, March 02, 2015
    Galectin-1 (gal-1) is an important molecule secreted by many tumors, which induces apoptosis in activated T-cells and promotes tumor angiogenesis, both of which phenomena facilitate successful ... read more
  • Immunofluroescent staining fixed tissues

    Immunofluroescent staining fixed tissues

    Jackson ImmunoResearch Laboratories, Inc.
    Friday, February 27, 2015
    In mouse models of Parkinson's Disease we use multiple primary antibodies to look at neuron populations following addition of MPTP. This secondary antibody has been used with anti-TH, anti-Map2, and ... read more
  • Anti-Human CD87 (UPAR) APC, 100 tests from eBioscience

    Anti-Human CD87 (UPAR) APC, 100 tests from eBioscience

    eBioscience
    Friday, February 27, 2015
    My project requires to test CD87 positive and negative cells with aptamers. Positive cells co-express mCherry but to confirm a receptor (CD87) which aptamers bind to these antibodies were purchased. ... read more
  • Secondary antibody to view neurons within midbrain

    Secondary antibody to view neurons within midbrain

    Jackson ImmunoResearch Laboratories, Inc.
    Thursday, February 26, 2015
    This antibody is used in conjunction with an anti-tyrosine hydroxylase antibody to view dopaminergic neurons in the SN in the MPTP-mouse model of PD. Following staining with primary and secondary ... read more
  • Detecting PDI by immunofluorescence

    Detecting PDI by immunofluorescence

    Santa Cruz Biotechnology, Inc.
    Thursday, February 26, 2015
    We are investigating the role of PDI during ER stress-induced apoptosis. One of our goals is to determine the subcellular localization of PDI during apoptosis. To detect its intracellular localization... read more
  • Anti-mouse CD8 for flow cytometry

    Anti-mouse CD8 for flow cytometry

    BioLegend
    Thursday, February 26, 2015
    I performed staining of mouse CD8 T cells, which were harvested from the splenocytes of a wild-type C57BL/6 mouse using bead based negative selection. The population of cells obtained was stained with... read more
  • Anti-Thy1.1 antibody for flow cytometry

    Anti-Thy1.1 antibody for flow cytometry

    BioLegend
    Thursday, February 26, 2015
    I transfected cells with a construct containing Thy1.1 as a reporter protein. Thy1.1 is a surface protein that is not usually expressed on mouse cells; so it is a great marker for detecting ... read more
  • Intracellular detection of TNF-alpha

    Intracellular detection of TNF-alpha

    BioLegend
    Wednesday, February 25, 2015
    This antibody was used for intracellular staining of TNFa on mouse CD8 T cells that were stimulated with OVA peptide. This seems to be a strong stimulation as most cells were positive for TNFa after 3... read more
  • Great control for immunoflorescence cell staining.

    Great control for immunoflorescence cell staining.

    Wednesday, February 25, 2015
    Used in staining of wild type trophoblast stem cells. It was a good control to compare to our mutant stem cells. We picked this product because it gave us consistently good results in our previous ... read more
  • Protein quantification from brain homogenate

    Protein quantification from brain homogenate

    Santa Cruz Biotechnology, Inc.
    Tuesday, February 24, 2015
    This antibody was used to look at the amount of tyrosine hydroxylase protein in brain homogenates of MPTP-intoxicated mice. Midbrain containing the SN and striatal areas of the brain of mice were ... read more
  • Great MeCP2 antibody for immunohistochemistry

    Great MeCP2 antibody for immunohistochemistry

    Cell Signaling Technology
    Monday, February 23, 2015
    My focus of work is studying the critical role of MeCP2 (methyl-CpG binding protein 2), which causes Rett Syndrome if mutated, in the development and function of the nervous system. I discovered a ... read more
  • Good antibody for immunohistochemistry and immunoblotting

    Good antibody for immunohistochemistry and immunoblotting

    EMD Millipore
    Monday, February 23, 2015
    I used this antibody for immunoblotting and immunohistochemistry on brain tissue samples. The antibody worked well for both techniques and is highly recommended. read more
  • Anti-tyrosine hydroxylase antibody for fresh frozen tissue

    Anti-tyrosine hydroxylase antibody for fresh frozen tissue

    EMD Millipore
    Friday, February 20, 2015
    This antibody is used to look for dopaminergic neurons within the SNpc and their termini within the striatum using the MPTP-mouse model of Parkinson’s disease. Following staining, tissues are mounted ... read more
  • Good loading control for immunoblotting

    Good loading control for immunoblotting

    EMD Millipore
    Friday, February 20, 2015
    For immunoblotting, a good loading control is always as important as proteins that we want to analyze. GAPDH, tubulin, and actin are the common loading controls used for immunoblotting. The anti-GAPDH... read more
  • Phospho vimentin S83

    Phospho vimentin S83

    Cell Signaling Technology
    Friday, February 20, 2015
    Vimentin is a cytoskeletal protein that is increasingly gaining importance in the field of cancer research. It is important to know the post-translational status of vimentin that will provide us novel... read more
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