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  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    Abcam
    Friday, April 17, 2015
    We are evaluating the changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific pathological processes. Bmal1 antibody from Abcam works well for ... read more
  • Sox11 from US Biological - works well for mouse retina

    Sox11 from US Biological - works well for mouse retina

    United States Biological
    Friday, April 17, 2015
    This antibody was used in a study investigating the role of transcription factor Sox11 in the adult mouse retina. Several antibodies were tested, and only this one worked. Publication forthcoming. read more
  • Very good Bmal1 antibody for immunoblotting

    Very good Bmal1 antibody for immunoblotting

    Abcam
    Thursday, April 16, 2015
    We are evaluating the changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific pathological processes. We used this antibody to determine levels of ... read more
  • DENV-2 Immunostaining with Anti -dengue Type 2

    DENV-2 Immunostaining with Anti -dengue Type 2

    United States Biological
    Wednesday, April 15, 2015
    Dengue virus (DENV-2) infected HeLa cells were immunostained for P- bodies and DENV-2. Cells grown on cover slips were washed in PBS, fixed in 4% paraformaldehyde, permeabilized with 0.5% Triton X-100... read more
  • Good secondary antibody

    Good secondary antibody

    Thermo Scientific Pierce Antibodies
    Wednesday, April 15, 2015
    This is a good secondary antibody that detects primary mouse antibodies and gives strong signal for fluorescent microscopy. read more
  • Good antibody for detecting Lamin A/C

    Good antibody for detecting Lamin A/C

    Santa Cruz Biotechnology, Inc.
    Tuesday, April 14, 2015
    I used this antibody to detect Lamin A/C in cytoplasmic (cyto.) and nuclear (nucl.) fractionated samples to assay the level of contamination in the different fractions. The Lamin A/C shows up in the ... read more
  • EPAC1 Western blot

    EPAC1 Western blot

    Cell Signaling Technology
    Tuesday, April 14, 2015
    Western blot of extracts of whole mouse brain was done using EPAC1 antibody. We wanted to see whether the protein was expressed in NHBE cells but I was only able to do a control which is the band in ... read more
  • Reliable CD45 antibody

    Reliable CD45 antibody

    eBioscience
    Tuesday, April 14, 2015
    We were trying to distinguish microglia and astrocytes, by flow cytometry, from mixed glial cultures cultured for 4-6 weeks from pup brains. We used this antibody for flow cytometry of brain and ... read more
  • Good antibody to detect Notch1

    Good antibody to detect Notch1

    Abcam
    Monday, April 13, 2015
    Used this antibody to detect Notch1 protein from whole cell lysates. Antibody recognized a single band at the molecular weight shown from the company and had little background signal. read more
  • Very good p16 antibody for immunoblotting

    Very good p16 antibody for immunoblotting

    Abcam
    Monday, April 13, 2015
    We are evaluating the basal level of DNA damage markers in wild type and clock deficient mutant mice to understand their role in specific pathological processes. We would like to compare stress-... read more
  • MUC5AC Immunfluorescence Staining in NHBE

    MUC5AC Immunfluorescence Staining in NHBE

    Santa Cruz Biotechnology, Inc.
    Monday, April 13, 2015
    This antibody was purchased to use as a marker of goblet cells in airway epithelial cells. read more
  • Good IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate

    Good IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate

    Thermo Scientific Pierce Antibodies
    Friday, April 10, 2015
    Primary mouse antibody against acetylated tubulin was detected. It gives a strong signal easily detectable in confocal microscopy and low background. read more
  • E-cadherin / CDH1 Antibody Immunofluorescence

    E-cadherin / CDH1 Antibody Immunofluorescence

    Thermo Scientific Pierce Antibodies
    Friday, April 10, 2015
    Cadherin formation is one of the steps among many in the process of airway epithelial cell differentiation. In our laboratory we use this antibody along with others to follow this process. read more
  • NR1D1 Antibody used for Western blotting

    NR1D1 Antibody used for Western blotting

    Novus Biologicals
    Friday, April 10, 2015
    We are evaluating changes in clock proteins in wild type and clock deficient mutant mice to understand their role in specific pathological processes. This product was used to determine levels of NR1D1... read more
  • ZO-1/TJP1 Immunofluorescence

    ZO-1/TJP1 Immunofluorescence

    Thermo Scientific Pierce Antibodies
    Thursday, April 09, 2015
    In our laboratory we have to track airway epithelial cells throughout differentiation. Tight junction formation is among those steps. read more
  • Very good HA antibody

    Very good HA antibody

    Cell Signaling Technology
    Thursday, April 09, 2015
    I used this anti-HA antibody to stain my N-terminal HA tagged NICD3 protein. NICD3 should primarily be in the nucleus and I see nice nuclear staining with the HA antibody. Overall, this is a good ... read more
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