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  • Use of Trypan blue stain to count cells

    Use of Trypan blue stain to count cells

    Monday, April 06, 2015
    Trypan Blue is used to stain cells which are then counted on a hemacytometer. read more
  • So happy I found this beta-actin antibody

    So happy I found this beta-actin antibody

    Tuesday, March 03, 2015
    Doing western blots became a lot easier using this pre-conjugated beta actin antibody. I use it on brain homogenate from MPTP-intoxicated mice to normalize protein concentrations loaded onto the gels. read more
  • Great control for immunoflorescence cell staining.

    Great control for immunoflorescence cell staining.

    Wednesday, February 25, 2015
    Used in staining of wild type trophoblast stem cells. It was a good control to compare to our mutant stem cells. We picked this product because it gave us consistently good results in our previous ... read more
  • Recommended cilia marker for immunofluorencence staining

    Recommended cilia marker for immunofluorencence staining

    Tuesday, February 17, 2015
    The aim of this study is to examine the cilia protein using fluorencent microcope. I choose this antibody as cilia marker because it alway give me great quality images and consistent result. read more
  • Easy Gel Extraction Kit

    Easy Gel Extraction Kit

    Friday, January 30, 2015
    Due to various factors, most PCR has primer dimers or misprimed product which needs to be removed for further downstream applications. Our goal was to obtain one single PCR Product in a regular PCR. ... read more
  • Great loading control antibody.

    Great loading control antibody.

    Monday, January 26, 2015
    Sigma's alpha-tubulin mouse monoclonal antibody is really great. While it is expensive, since this is a fairly ubiquitously expressed protein, you can easily get away with dilutions of 1:5000 and ... read more
  • The best anti-Lis1 antibody for Western Blot

    The best anti-Lis1 antibody for Western Blot

    Monday, December 29, 2014
    This antibody has been extensively used to detect endogenous and overexpressed Lis1 in wild type, Lis1 knock-out and TgLis1 mouse tissues to confirm identity of transgenic samples. It has also been ... read more
  • REDTaq ReadyMix PCR Mix for easy standardization of PCR - Uncredited Review*

    REDTaq ReadyMix PCR Mix for easy standardization of PCR - Uncredited Review*

    Monday, December 29, 2014
    Our project aimed at isolating homologs from genomic and cDNA of a plant whose DNA template was particularly difficult to work with. With a specific and difficult target to amplify the homologs, ... read more
  • Alpha-actinin antibody for cardiomyocyte staining

    Alpha-actinin antibody for cardiomyocyte staining

    Wednesday, December 10, 2014
    Excellent antibody to characterize human iPSC-derived cardiomyocytes in cell culture. read more
  • Acetylated tubulin Ab

    Acetylated tubulin Ab

    Tuesday, November 25, 2014
    I have used this on non-model organisms for staining neurons and worked great! read more
  • HP1(alpha) Detection in Chromocenters

    HP1(alpha) Detection in Chromocenters

    Monday, June 02, 2014
    HP1 proteins bind to DNA regions to control gene expression and heterochromatin formation and maintenance. The amount of chromocenters detected by HP1 staining is a clear indication of alterations of ... read more
  • PEI - An Inexpensive Transfection Reagent

    PEI - An Inexpensive Transfection Reagent

    Tuesday, March 25, 2014
    My research involves making lentiviral vectors pseudotyped with vesicular stomatitis virus envelop glycoprotein (VSV-G). In this protocol, I transfect HEK-293T cells with 3 plasmids; one for ... read more
  • Antibody for HA Tagging a Protein

    Antibody for HA Tagging a Protein

    Friday, March 21, 2014
    Hemagglutinin (HA) epitope tag, or HA tag, is one of the most usual ways to mark and detect a protein in vivo. Good antibody for HA tag is essential to many popular laboratory techniques as WB, IF or ... read more
  • Loading Control for WB: Tubulin

    Loading Control for WB: Tubulin

    Monday, January 27, 2014
    Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins and a sixth ... read more
  • Alpha Amylase Detection in Pancreas

    Alpha Amylase Detection in Pancreas

    Friday, January 24, 2014
    a-Amylase is a protein enzyme EC 3.2.1.1 that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Pancreatic a-amylase randomly ... read more
  • Insulin Detection in Pancreas

    Insulin Detection in Pancreas

    Wednesday, December 25, 2013
    Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. Insulin causes cells in the liver, skeletal muscles, and... read more
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