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  • BACH2 copy number analysis using Life Technologies TaqMan Assays

    BACH2 copy number analysis using Life Technologies TaqMan Assays

    Tuesday, September 30, 2014
    I am interested in copy number variations of particular genes in B-cell lymphoma. I have used the Life Technologies Copy Number Assays to validate SNP Array data. read more
  • PrimeSTAR GXL DNA Polymerase Amplifies Genomic DNA from Crude C. elegans Extracts

    PrimeSTAR GXL DNA Polymerase

    Takara Bio
    Thursday, February 20, 2014
    For this project, my goal was to use a PCR-based strategy to genotype C. elegans strains in order to confirm the presence or absence of specific mutations. Because I was genotyping anywhere from 4-16... read more
  • SapphireAmp Fast PCR Master Mix

    SapphireAmp Fast PCR Master Mix

    Takara Bio
    Thursday, February 06, 2014
    This reagent is designed to rapidly set up a hot start PCR. This made it ideal for large scale genotyping to assess a single nucleotide polymorphism of interest. The reagents were successful in a ... read more
  • Verso cDNA synthesis Kit from Thermo Scientific

    cDNA Synth Kit for qRT-PCR Analysis of Gene Expression

    Wednesday, May 15, 2013
    Selection of the proper cDNA synthesis kit is critical for detailed analysis of quantitative gene expression. The Verso enzyme cDNA synthesis kit is widely used for cDNA synthesis, due to its proven ... read more
  • Gene Pattern Software from Broad Institute of MIT and Harvard

    Gene Pattern Software from Broad Institute of MIT and Harvard

    Monday, March 18, 2013
    We are looking at gene expression levels of a number of genes using real-time PCR in an attempt to see whether these can be used for disease diagnosis or monitoring of disease activity. GenePattern ... read more
  • TargetAmp 2-Round aRNA Amplification Kit 2.0 from Epicentre Biotechnologies

    TargetAmp RNA Amplification Kit from Epicentre Biotechnologies

    Wednesday, March 06, 2013
    The aim of this kit is to amplify total RNA when you have limited amounts of starting material. In our case, we were looking for tumor cells which were present in patients’ blood . After isolating ... read more
  • CFX Connect Real-Time PCR Detection System from Bio-Rad

    CFX Connect from Bio-Rad

    Bio-Rad
    Tuesday, March 05, 2013
    The Bio-Rad CFX Connect Real-Time PCR Detection System is a standard, 96 well quantitative PCR detection system that can detect up to two fluorophores at the same time. Detection thresholds for ... read more
  • SYBR® Green JumpStart™ Taq ReadyMix™ from Sigma-Aldrich

    SYBR® Green JumpStart™ Taq ReadyMix™ from Sigma-Aldrich

    Wednesday, August 11, 2010
    Quantitative real-time PCR (qPCR) is fast replacing conventional reverse transcriptase PCR in many molecular biology labs, increasingly reflected by high quality journals adjusting their requirements ... read more
  • iQ SYBR Green Real-Time PCR Supermix from BioRad

    iQ SYBR Green Real-Time PCR Supermix from BioRad

    Bio-Rad
    Tuesday, April 20, 2010
    Bio-Rad offers the iQ SYBR Green Supermix which contains dNTPs, 50 U/ml iTaq DNA polymerase, 6 mM MgCl2, SYBR Green I, 20 nM fluorescein. To this mixture, just add your primers and template DNA to ... read more
  • MasterCycler RealPlex4 From Eppendorf

    MasterCycler RealPlex4 From Eppendorf

    Eppendorf North America
    Wednesday, May 14, 2008
    This is Eppendorf’s big-brother qPCR platform, which in many ways is similar to the RealPlex2. Like the RealPlex2, this machine is small, solid and very quiet. It has a small footprint (40cm x 24cm ... read more
  • Mastercycler® RealPlex2 From Eppendorf

    Mastercycler® RealPlex2 From Eppendorf

    Eppendorf North America
    Thursday, March 20, 2008
    The Eppendorf Mastercycler® RealPlex2 is a small, robustly designed instrument for real-time PCR. The machine features, like some other Mastercyler devices from Eppendorf, a special 96-well...... read more
  • QuantiTect Primer Assays (for use in real-time RT-PCR with SYBR Green detection) From Qiagen

    QuantiTect Primer Assays (for use in real-time RT-PCR with SYBR Green detection) From Qiagen

    Friday, February 29, 2008
    Polymerase chain reaction (PCR) is used to amplify a fragment of DNA via enzymatic replication across several orders of magnitude, generating millions or more copies of the initial sequence. PCR is ... read more
  • LA Taq™ With GC Buffer From Takara

    LA Taq™ With GC Buffer From Takara

    Takara Bio
    Friday, October 05, 2007
    TaKaRa LA (Long and accurate) Taq™ is a thermostable DNA polymerase having 3'-5' exonuclease activity (proof reading activity) that is capable of, under general PCR conditions, higher efficiency and ... read more
  • Chaperone Plasmid Set From Takara Mirus Bio

    Chaperone Plasmid Set From Takara Mirus Bio

    Takara Bio
    Friday, January 26, 2007
    As with many other laboratories, we rely heavily on large-scale recombinant protein expression in our effort to conduct genetic and proteomic analysis of parasite-host cell interactions. There are ... read more
  • DNA Ligation Kit, Version 1 From Takara

    DNA Ligation Kit, Version 1 From Takara

    Takara Bio
    Monday, January 08, 2007
    How did I get to know the DNA Ligation Kit from Takara? Around 2 years ago, I was trying to do a cloning and I was stuck at the ligation step. At first, I was using the regular T4 DNA ligase and ... read more
  • SYBR® Premix Ex Taq™ (Perfect Real Time) From Takara Mirus Bio

    SYBR® Premix Ex Taq™ (Perfect Real Time) From Takara Mirus Bio

    Takara Bio
    Monday, January 08, 2007
    SYBR® Premix Ex Taq™ (Perfect Real Time) is a 2X concentration of premixed reagent which includes Takara’s Ex Taq™ HS and SYBR® Green I. The Ex Taq™ HS is an enzyme for hot start PCR... read more
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