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  • PEI - An Inexpensive Transfection Reagent

    PEI - An Inexpensive Transfection Reagent

    Tuesday, March 25, 2014
    My research involves making lentiviral vectors pseudotyped with vesicular stomatitis virus envelop glycoprotein (VSV-G). In this protocol, I transfect HEK-293T cells with 3 plasmids; one for ... read more
  • PCR Product Cleanup Using the QIAquick PCR Purification Kit

    PCR Product Cleanup

    QIAGEN - Sample & Assay Technologies
    Friday, January 31, 2014
    I have used the Qiagen QIAquick PCR Purification kit to successfully purify DNA from PCR products, thus removing primers, nucleotides, polymerases etc. using the QIAquick spin columns in a ... read more
  • PolyJet DNA In Vitro Transfection Reagent from SignaGen Laboratories

    PolyJet DNA In Vitro Transfection Reagent from SignaGen Laboratories

    Friday, July 27, 2012
    PolyJet works very well, especially in HEK293 series cells, such as HEK293, HEK293T, HEK293FT. Compared to other transfection reagents, it is much less expensive but shows same efficiency of ... read more
  • Extracellular Matrix and Adhesion Molecules PCR Array from SA Biosciences (a Qiagen company)

    Extracellular Matrix and Adhesion Molecules PCR Array from SA Biosciences (a Qiagen company)

    QIAGEN - Sample & Assay Technologies
    Tuesday, January 11, 2011
    Microarray technology has been in use in the scientific community for the past few decades. This technique allows for obtaining data in an entire genome. Although enormous amounts of data can be ... read more
  • Human Cell Cycle PCR Array from SABiosciences Corporation

    Human Cell Cycle PCR Array from SABiosciences Corporation

    QIAGEN - Sample & Assay Technologies
    Monday, February 22, 2010
    Several techniques have been developed for in vitro analysis of cell cycle, however, for in vivo studies, very few options are available especially when the samples are freshly frozen (FF) human ... read more
  • PolyFect Transfection Reagent From Qiagen

    PolyFect Transfection Reagent From Qiagen

    Thursday, February 28, 2008
    In our experiment, I used Qiagen’s QIAEXII Gel Extraction Kit (Cat. No. 20021) to prepare the DNA prior to transfection so that it was clear of bacterial endotoxins. I used the “desalting protocol” ... read more
  • GeneJuice® Transfection Reagent From Novagen

    GeneJuice® Transfection Reagent From Novagen

    Novagen
    Thursday, February 21, 2008
    Transfection and expression of altered genes into cell lines are indispensable parts of genetic studies. There are many different methods for introducing genetic material into eukaryotic cells, ... read more
  • Lipofectamine™ 2000 From Invitrogen

    Lipofectamine™ 2000 From Invitrogen

    Life Technologies
    Tuesday, September 25, 2007
    A common method used in almost every lab these days is transient transfection of cell lines followed by some form of output (Western blot, reporter gene assays, etc.). In a majority of cases, one ... read more
  • PLUS™ Reagent From Invitrogen

    PLUS™ Reagent From Invitrogen

    Life Technologies
    Tuesday, September 25, 2007
    Efficient and effective transfection reagents are essential for protein expression in eukaryotic cells. Recent years have seen a rapid expansion in the number and type of transfection reagents ... read more
  • Lipofectamine™ LTX Transfection Reagent From Invitrogen

    Lipofectamine™ LTX Transfection Reagent From Invitrogen

    Life Technologies
    Tuesday, September 25, 2007
    Invitrogen have added a new product, Lipofectamine™ LTX, to their extensive range of transfection products. Following on from other products in the Lipofectamine range, Lipofectamine™ LTX is a lipid ... read more
  • RT2 Profiler™ PCR Array From SuperArray Biosciences

    RT2 Profiler™ PCR Array From SuperArray Biosciences

    QIAGEN - Sample & Assay Technologies
    Tuesday, September 11, 2007
    Real-time reverse-transcription PCR is the most sensitive and reliable method for gene expression analysis. However, using a conventional approach, real-time PCR assays can only monitor a few genes at... read more
  • GEArray Q Series Human Endothelial Cell Biology Gene Array From SuperArray

    GEArray Q Series Human Endothelial Cell Biology Gene Array From SuperArray

    QIAGEN - Sample & Assay Technologies
    Tuesday, April 10, 2007
    The GEArray Q Series Human Endothelial Cell Biology Gene Array is designed for analysis of the expression of genes associated with the major functions of endothelial cells, those being permissibility ... read more
  • AmpoLabeling-LPR Kit From SuperArray

    AmpoLabeling-LPR Kit From SuperArray

    QIAGEN - Sample & Assay Technologies
    Friday, March 30, 2007
    The AmpoLabeling-LPR Kit is designed to enzymatically convert experimental RNA into labeled cDNA target for hybridization to the cDNA GEArray from SuperArray. The system is based on a linear ... read more
  • Chemiluminescent Detection Kit From SuperArray

    Chemiluminescent Detection Kit From SuperArray

    QIAGEN - Sample & Assay Technologies
    Friday, March 30, 2007
    The Chemiluminescent Array Detection Kit is designed to allow the detection of biotinylated probes or targets hybridized to a GEArray cDNA array. The kit contains all the reagents necessary... read more
  • Lipofectamine™ 2000 Transfection Reagent From Invitrogen

    Lipofectamine™ 2000 Transfection Reagent From Invitrogen

    Life Technologies
    Tuesday, March 27, 2007
    Lipofectamine™ 2000 Reagent is a lipid-derived reagent designed to deliver exogenic nucleic acids (DNA and/or RNA) into eukaryotic cells, either adherent or in suspension. Thee two most important ... read more
  • Lipofectamine™ 2000 from Invitrogen

    Lipofectamine™ 2000 from Invitrogen

    Life Technologies
    Friday, December 01, 2006
    Transfection reagents are big business. The ability to express exogenous genes within eukaryotic cells is very popular and companies are falling over themselves marketing these products, claiming to ... read more
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