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  • Akt Detection in MDCK Cells

    Akt Detection in MDCK Cells

    Cell Signaling Technology
    Tuesday, January 21, 2014
    Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function ... read more
  • Akt Pathway Activation Analysis

    Akt Pathway Activation Analysis

    Cell Signaling Technology
    Wednesday, January 22, 2014
    Since its initial discovery as a proto-oncogene, the serine/threonine kinase Akt (also known as protein kinase B or PKB) has become a major focus of attention because of its critical regulatory role ... read more
  • Antibody Sampler Kits From Cell Signaling Inc.

    Antibody Sampler Kits From Cell Signaling Inc.

    Cell Signaling Technology
    Wednesday, December 20, 2006
    The availability of quality antibodies is essential for all researchers in cell biology or molecular biology. However, antibodies are expensive reagents and it is essential to know which antibody ... read more
  • Calcium activity by measuring Phospho-CaMKII

    Calcium activity by measuring Phospho-CaMKII

    Cell Signaling Technology
    Tuesday, September 24, 2013
    Purpose of experiment was to look at intracellular calcium activity by measuring the activation of p- CaMKII. between two cell populations ( WT and KO MEFS). Lane 1, 4: Control Lane 2, 5: ... read more
  • Cell Signaling Technology's Cleaved Caspase-3 (D175) Antibody

    Cell Signaling Technology's Cleaved Caspase-3 (D175) Antibody

    Cell Signaling Technology
    Tuesday, October 12, 2004
    Caspases are a group of cysteine proteases that cleave proteins after aspartic acid residues. To date, there are 14 known caspases that are divided into two main classes: initiators (or activators) ... read more
  • Clathrin Heavy Chain Antibody

    Clathrin Heavy Chain Antibody

    Cell Signaling Technology
    Monday, March 10, 2014
    A clean antibody to detect Clathrin heavy chain. High specificity but sensitivity is okay, load 30-40 ug total protein to get a strong signal. read more
  • Cleaved PARP Antibody from Cell Signaling Technology, used in Western Blotting

    Cleaved PARP Antibody from Cell Signaling Technology, used in Western Blotting

    Cell Signaling Technology
    Saturday, March 31, 2012
    Clean staining on Western blot of whole cell lysates with anti-PARP antibody. read more
  • DirectPCR Lysis Reagent From Viagen

    DirectPCR Lysis Reagent From Viagen

    Viagen
    Friday, April 25, 2008
    Viagen DirectPCR Lysis Reagent is a buffer used to lyse animal tissue and release the DNA for future applications, such as PCR. It promises to contain inhibitors of... read more
  • E-Cadherin Monoclonal Antibody from Cell Signaling Technology

    E-Cadherin mAb from Cell Signaling Technology

    Cell Signaling Technology
    Thursday, January 24, 2013
    Anti-E-cadherin monoclonal antibody is used in Western blotting and IHC with good results. read more
  • Excellent Anti Phospho ERK1/2 Antibody for WB

    Excellent Anti Phospho ERK1/2 Antibody for WB

    Cell Signaling Technology
    Wednesday, January 08, 2014
    The goal was to test ERK1/2 activation in primary mouse cardiac cells after growth factors (FGF, PDGF and EGF) stimulation. As shown on the Western blot, 5 minutes of growth factor stimulation led to ... read more
  • Great Cleaved Caspase 3 Antibody

    Great Cleaved Caspase 3 Antibody

    Cell Signaling Technology
    Friday, February 07, 2014
    Experiments were aimed at detecting apoptotic cells in postnatal mouse brain tissue. Cleaved Caspase 3 is a well-known marker for cells undergoing apoptosis in the caspase-dependent pathway. read more
  • IRF3 Activation Measurement During Viral Infections

    IRF3 Activation Measurement During Viral Infections

    Cell Signaling Technology
    Thursday, October 31, 2013
    The goal of this experiment was to compare antiviral signalling between two sets of MEFS (WT vs KO). MEFs were infected with Sendai virus and antiviral signalling was monitored by measuring the ... read more
  • LC3B Antibody for Tissue Staining

    LC3B Antibody for Tissue Staining

    Cell Signaling Technology
    Wednesday, February 26, 2014
    I have tried several antibodies to stain xenograft tisse for LC3B- a marker for autophagososmes. The cell signaling LC3B D11 antibody works great with almost no background, very specific staining. It ... read more
  • Pathscan® Phospho-Histone H3 (Ser10) Sandwich ELISA Kit From Cell Signaling Technologies

    Pathscan® Phospho-Histone H3 (Ser10) Sandwich ELISA Kit From Cell Signaling Technologies

    Cell Signaling Technology
    Thursday, February 21, 2008
    Cell Signaling Technologies’ sandwich ELISA kit to measure phosphorylated histone H3 (Ser10) is a solid phase sandwich ELISA. This ELISA is designed to measure phospho- histone H3 in cell lysates. The... read more
  • Pathscan® Phosphor-HSP27 (Ser78) Sandwich ELISA Kit From Cell Signaling Technology

    Pathscan® Phosphor-HSP27 (Ser78) Sandwich ELISA Kit From Cell Signaling Technology

    Cell Signaling Technology
    Wednesday, July 12, 2006
    Cell Signaling Technology’s Sandwich ELISA Kit for measurement of phosphorylated HSP27 (Ser78) is a solid phase sandwich ELISA. This ELISA is designed to measure phospho-HSP27 in cell lysates. The kit... read more
  • PTEN Detection by WB

    PTEN detection by WB

    Cell Signaling Technology
    Thursday, January 23, 2014
    PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of ... read more
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