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  • QuikChangeII Mutagenesis Kit from Agilent  Technologies

    QuikChangeII Site-Directed Mutagenesis Kit from Agilent Technologies

    Friday, March 01, 2013
    Overall, the kit allows the mutagenesis of plasmid vectors of interest, including single or multiple (but relatively adjacent) nucleotide substitutions, deletions and insertions. The protocol is ... read more
  • Maxwell 16 Research System from Promega

    Maxwell 16 Research System from Promega

    Thursday, February 28, 2013
    Promega has developed the Maxwell® 16 Research System as a high throughput, and opportunity cost solution to address the efficiency demands of the research laboratory. The System can accommodate up ... read more
  • Mini-Protean TGX Stain-Free Precast Gels from Bio-Rad

    Mini-Protean Precast Gels from Bio-Rad

    Tuesday, February 26, 2013
    We work with high molecular weight proteins on a regular basis, as well as low molecular weight proteins, so we use gradient gels. We use these gels for Western blotting, coomassie staining, and ... read more
  • PTEN XP Rabbit Monoclonal Antibody (D4.3) from Cell Signaling Technology

    PTEN Antibody from Cell Signaling Technology

    Monday, February 25, 2013
    PTEN is a tumor suppressor implicated in a wide variety of human cancers. It is a major negative regulator of the PI3K/Akt signaling pathway and also regulates p53 protein levels and activity. ... read more
  • Eporator from Eppendorf

    Eporator from Eppendorf

    Thursday, February 21, 2013
    We use Gram positive and Gram negative bacteria for the transformation with DNA constructs encoding fluorescent proteins. We prefer electroporation, which is quicker and simpler than other methods. ... read more
  • Native Protein Deglycosylation Kit from Sigma-Aldrich

    Deglycosylation Kit from Sigma-Aldrich

    Tuesday, February 19, 2013
    The Native Protein Deglycosylation Kit is used to remove N-linked oligosaccharides from glycoproteins under native conditions as opposed to more traditional methods. Glycosylation protein ... read more
  • Anti-GAPDH Antibody from Abcam

    Anti-GAPDH Antibody from Abcam

    Monday, February 18, 2013
    This GAPDH antibody is a good choice for a reference gene since it is of good quality and is extremely cost-effective. read more
  • Anti-Iba-1 Polyclonal Rabbit Antibody from Wako

    Anti-Iba-1 Antibody from Wako

    Friday, February 15, 2013
    For the detection of microglia in mouse and human brain and spinal cord tissues, Iba-1 has been identified as a good marker candidate. Indeed, Iba-1 is specifically expressed in macrophages/microglia ... read more
  • KAPA SYBR FAST Universal 2X qPCR Master Mix from Kapa Biosystems

    SYBR Fast qPCR Mix from Kapa Biosystems

    Thursday, February 14, 2013
    In our lab, we perform a great deal of qPCR on DNA isolated from mouse stool samples. The purpose being to determine intestinal micro flora changes in the setting of our experiment. Speed and cost ... read more
  • Trans-blot Turbo System from Bio-Rad

    Trans-blot Turbo System from Bio-Rad

    Wednesday, February 13, 2013
    Our research in the lab relies heavily on in vitro biochemical assays, especially routine SDS-PAGE gel electrophoresis and Western blotting. Transfers using conventional tank and semi-dry Western ... read more
  • mirVana microRNA Isolation Kit from Life Technologies

    mirVana microRNA Isolation Kit from Life Technologies

    Tuesday, February 12, 2013
    I use this kit to extract total RNA (including microRNA) from a range of cultured and primary cells. I then use these RNA samples for reverse transcription and TaqMan® quantitative PCR. read more
  • GCET1 Mouse Monoclonal Antibody (RAM341) from Abcam

    GCET1 mAb from Abcam

    Monday, February 11, 2013
    GCET1 protein has been identified as playing an important role in germinal center B-cell physiology and as such, is deemed a marker for germinal center-like B-cells. I am using the GCET1 antibody to ... read more
  • SepMate-50 from StemCell Technologies

    SepMate-50 from StemCell Technologies

    Friday, February 08, 2013
    SepMate-50 uses apparently simple technology to solve a common problem for our lab. We have been able to save time and cut down sample loss due to poor PBMC separation. We are able to recover as many ... read more
  • Cellometer Auto T4 Cell Counter from Nexcelom

    Cellometer Auto T4 from Nexcelom

    Thursday, February 07, 2013
    We utilize a number of different tissue culture models including epithelial cells (lung and GI) and suspension cells (leukocytes), both primary cells and cell lines. We measure gene expression ... read more
  • Wizard SV Gel and PCR Clean-Up System from Promega

    Wizard SV Gel and PCR Clean-Up from Promega

    Wednesday, February 06, 2013
    The Wizard SV Gel and PCR Clean-Up System performed very well; it was fast, reliable and inexpensive. The protocol was simple and resulted in high quality DNA, as evidenced by spectrophotometry ... read more
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