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  • Great antibody for detecting human CD4 expression

    Great antibody for detecting human CD4 expression

    BioLegend
    Friday, March 06, 2015
    This antibody was used for detecting human CD4 expression on human CD45 gated cells isolated from humanized mice. The results showed a clear CD4 positive and negative population which confirms the ... read more
  • Anti-PIAS1 (D33A7) XP

    Anti-PIAS1 (D33A7) XP

    Cell Signaling Technology
    Friday, March 06, 2015
    I have tried several antibodies to detect PIAS1 (endogenous or in condition of over-expression) in cells. So far, this antibody is the best one for both western blot and immunofluorescence analysis. read more
  • Donkey Anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 647 Conjugate

    Donkey Anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 647 Conjugate

    Life Technologies
    Thursday, March 05, 2015
    This is a great product to do immunofluorescence. We study primary cells (airway epithelial cells) in our lab and can use several different donkey alexas with different fluorophore conjugations in a ... read more
  • One of the few LAP antibodies that work

    One of the few LAP antibodies that work

    BioLegend
    Thursday, March 05, 2015
    Our group is interested in regulatory Th3 cells that express membrane bound TGF-beta. The only way to detect these cells is by surface staining of the TGF-beta complex by antibodies recognizing ... read more
  • Anti SAA4 (S1002-60A) for Sandwich ELISA

    Anti SAA4 (S1002-60A) for Sandwich ELISA

    United States Biological
    Wednesday, March 04, 2015
    I used this antibody for sandwich ELISA. I chose this product because I have purchased other antibodies from US Biological in the past and they have worked. This time I bought a couple of antibodies ... read more
  • NeuN Antibody for Western Blot

    NeuN Antibody for Western Blot

    EMD Millipore
    Wednesday, March 04, 2015
    This product was used to monitor expression levels of NeuN upon N2A cell differentiation to study the Differentiation of neuroblstoma (N2A cells) to mature neurons in serum deprivation. read more
  • Tubulin beta-III for Immunocytochemistry

    Tubulin beta-III for Immunocytochemistry

    EMD Millipore
    Wednesday, March 04, 2015
    This product was used to stain the differentiated neuroblastoma cells (N2A cells) to study the differential neurite out growth after treating with neurotrophic compounds. read more
  • So happy I found this beta-actin antibody

    So happy I found this beta-actin antibody

    Tuesday, March 03, 2015
    Doing western blots became a lot easier using this pre-conjugated beta actin antibody. I use it on brain homogenate from MPTP-intoxicated mice to normalize protein concentrations loaded onto the gels. read more
  • Intracellular detection of IFN-gamma

    Intracellular detection of IFN-gamma

    BioLegend
    Monday, March 02, 2015
    Mouse transgenic OT-1 cells were stimulated with their cognate peptide: OVA for 3 days. On the last day, cells were re-stimulated with PMA/ionomycin and treated with Brefeldin A to allow accumulation ... read more
  • Galectin-1 Immunofluorescence Detection

    Galectin-1 Immunofluorescence Detection

    R&D Systems
    Monday, March 02, 2015
    Galectin-1 (gal-1) is an important molecule secreted by many tumors, which induces apoptosis in activated T-cells and promotes tumor angiogenesis, both of which phenomena facilitate successful ... read more
  • Immunofluroescent staining fixed tissues

    Immunofluroescent staining fixed tissues

    Jackson ImmunoResearch Laboratories, Inc.
    Friday, February 27, 2015
    In mouse models of Parkinson's Disease we use multiple primary antibodies to look at neuron populations following addition of MPTP. This secondary antibody has been used with anti-TH, anti-Map2, and ... read more
  • Anti-Human CD87 (UPAR) APC, 100 tests from eBioscience

    Anti-Human CD87 (UPAR) APC, 100 tests from eBioscience

    eBioscience
    Friday, February 27, 2015
    My project requires to test CD87 positive and negative cells with aptamers. Positive cells co-express mCherry but to confirm a receptor (CD87) which aptamers bind to these antibodies were purchased. ... read more
  • Anti-mouse CD8 for flow cytometry

    Anti-mouse CD8 for flow cytometry

    BioLegend
    Thursday, February 26, 2015
    I performed staining of mouse CD8 T cells, which were harvested from the splenocytes of a wild-type C57BL/6 mouse using bead based negative selection. The population of cells obtained was stained with... read more
  • Anti-Thy1.1 antibody for flow cytometry

    Anti-Thy1.1 antibody for flow cytometry

    BioLegend
    Thursday, February 26, 2015
    I transfected cells with a construct containing Thy1.1 as a reporter protein. Thy1.1 is a surface protein that is not usually expressed on mouse cells; so it is a great marker for detecting ... read more
  • Detecting PDI by immunofluorescence

    Detecting PDI by immunofluorescence

    Santa Cruz Biotechnology, Inc.
    Thursday, February 26, 2015
    We are investigating the role of PDI during ER stress-induced apoptosis. One of our goals is to determine the subcellular localization of PDI during apoptosis. To detect its intracellular localization... read more
  • Secondary antibody to view neurons within midbrain

    Secondary antibody to view neurons within midbrain

    Jackson ImmunoResearch Laboratories, Inc.
    Thursday, February 26, 2015
    This antibody is used in conjunction with an anti-tyrosine hydroxylase antibody to view dopaminergic neurons in the SN in the MPTP-mouse model of PD. Following staining with primary and secondary ... read more
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