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  • PEI - An Inexpensive Transfection Reagent

    PEI - An Inexpensive Transfection Reagent

    Tuesday, March 25, 2014
    My research involves making lentiviral vectors pseudotyped with vesicular stomatitis virus envelop glycoprotein (VSV-G). In this protocol, I transfect HEK-293T cells with 3 plasmids; one for ... read more
  • PrimeSTAR GXL DNA Polymerase Amplifies Genomic DNA from Crude C. elegans Extracts

    PrimeSTAR GXL DNA Polymerase

    Takara Bio
    Thursday, February 20, 2014
    For this project, my goal was to use a PCR-based strategy to genotype C. elegans strains in order to confirm the presence or absence of specific mutations. Because I was genotyping anywhere from 4-16... read more
  • SapphireAmp Fast PCR Master Mix

    SapphireAmp Fast PCR Master Mix

    Takara Bio
    Thursday, February 06, 2014
    This reagent is designed to rapidly set up a hot start PCR. This made it ideal for large scale genotyping to assess a single nucleotide polymorphism of interest. The reagents were successful in a ... read more
  • PolyJet DNA In Vitro Transfection Reagent from SignaGen Laboratories

    PolyJet DNA In Vitro Transfection Reagent from SignaGen Laboratories

    Friday, July 27, 2012
    PolyJet works very well, especially in HEK293 series cells, such as HEK293, HEK293T, HEK293FT. Compared to other transfection reagents, it is much less expensive but shows same efficiency of ... read more
  • Targefect-RAW From Targeting Systems

    Targefect-RAW From Targeting Systems

    Targeting Systems
    Monday, June 08, 2009
    Targefect-RAW is a new reagent from Targeting Systems specifically designed for the transfection of the RAW 264.7 macrophage-like cell line. Although these cells are quite useful for studying innate ... read more
  • JetPEI™ Transfection Reagent From Polyplus Transfection

    JetPEI™ Transfection Reagent From Polyplus Transfection

    Wednesday, May 06, 2009
    Transfection refers to the introduction of foreign DNA into a eukaryotic cell, and can be achieved by simple uptake of a precipitate mixture of calcium phosphate and the DNA to be transfected. ... read more
  • LA Taq™ With GC Buffer From Takara

    LA Taq™ With GC Buffer From Takara

    Takara Bio
    Friday, October 05, 2007
    TaKaRa LA (Long and accurate) Taq™ is a thermostable DNA polymerase having 3'-5' exonuclease activity (proof reading activity) that is capable of, under general PCR conditions, higher efficiency and ... read more
  • Chaperone Plasmid Set From Takara Mirus Bio

    Chaperone Plasmid Set From Takara Mirus Bio

    Takara Bio
    Friday, January 26, 2007
    As with many other laboratories, we rely heavily on large-scale recombinant protein expression in our effort to conduct genetic and proteomic analysis of parasite-host cell interactions. There are ... read more
  • DNA Ligation Kit, Version 1 From Takara

    DNA Ligation Kit, Version 1 From Takara

    Takara Bio
    Monday, January 08, 2007
    How did I get to know the DNA Ligation Kit from Takara? Around 2 years ago, I was trying to do a cloning and I was stuck at the ligation step. At first, I was using the regular T4 DNA ligase and ... read more
  • SYBR® Premix Ex Taq™ (Perfect Real Time) From Takara Mirus Bio

    SYBR® Premix Ex Taq™ (Perfect Real Time) From Takara Mirus Bio

    Takara Bio
    Monday, January 08, 2007
    SYBR® Premix Ex Taq™ (Perfect Real Time) is a 2X concentration of premixed reagent which includes Takara’s Ex Taq™ HS and SYBR® Green I. The Ex Taq™ HS is an enzyme for hot start PCR... read more
  • Single Tube PCR Kit From Takara Bio

    Single Tube PCR Kit From Takara Bio

    Takara Bio
    Wednesday, June 21, 2006
    The Single-Tube PCR Kit from Takara allows nucleic acid extraction and the subsequent PCR to occur in a single tube in a short amount of time. It can be used with small volumes (3 to 10ul) of whole ... read more
  • PrimeSTAR™ HS DNA Polymerase

    PrimeSTAR™ HS DNA Polymerase

    Takara Bio
    Wednesday, May 24, 2006
    The reduced mutation frequency of PrimeStar™ is attributed to the enzyme’s strong exonuclease activity, resulting in 12 errors/250,000 bp. Takara also claims that PrimeStar™ has a higher efficiency ... read more
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