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  • Great antibody to distinguish between activated and non-activated platelets

    Great antibody to distinguish between activated and non-activated platelets

    BioLegend
    Friday, March 27, 2015
    This antibody was used to determine if washed human platelets were activated after exposure to thrombin. After purification, platelets were incubated with or without 1UI/ml thrombin for 30mins at 37 ... read more
  • Great antibody for detecting human CD42b

    Great antibody for detecting human CD42b

    BioLegend
    Thursday, March 26, 2015
    This antibody was used to select and gate on human platelets that were isolated by a method of centrifugation and washing from a sample of peripheral human blood. After purification, the cells were ... read more
  • Superb CD166 antibody!

    Superb CD166 antibody!

    BioLegend
    Tuesday, March 24, 2015
    Detecting cancer stem cells in never easy. Scientists usually use a selection of antibodies. Our lab chose CD166 (which has been recently identified as a potential cancer stem cell marker). We used it... read more
  • Th2 Cytokine IL-4 in House dust mite model

    Th2 Cytokine IL-4 in House dust mite model

    BioLegend
    Thursday, March 19, 2015
    The aim of my research was to compare WT and KO mice to see a change in Th2 signaling during a housedustmite (HDM) allergy model. Thus, I wanted to look for the important TH2 cytokine IL-4 via flow ... read more
  • Good Quality TCR Beta Chain Antibody

    Good Quality TCR Beta Chain Antibody

    BioLegend
    Friday, March 13, 2015
    Our lab uses TCR beta antibody and CD1d tetramer to identify our population of interest. This antibody is easy to optimize and stained our target cells very well read more
  • Very Good Anti-Human CD45 antibody

    Very Good Anti-Human CD45 antibody

    BioLegend
    Monday, March 09, 2015
    Single cell suspensions prepared from the spleen of human PBMC reconstituted mice was used for flow cytometric analysis. I have used this antibody to separate human cells from the spleen of these ... read more
  • Very Good Anti-Human CD8 antibody

    Very Good Anti-Human CD8 antibody

    BioLegend
    Monday, March 09, 2015
    I have used this antibody to analyze the fraction of CD8+ T cells isolated from splenocytes of humanized mice. The staining looks good with two separate populations of CD8 positive and negative cells. read more
  • Great antibody for detecting human CD4 expression

    Great antibody for detecting human CD4 expression

    BioLegend
    Friday, March 06, 2015
    This antibody was used for detecting human CD4 expression on human CD45 gated cells isolated from humanized mice. The results showed a clear CD4 positive and negative population which confirms the ... read more
  • One of the few LAP antibodies that work

    One of the few LAP antibodies that work

    BioLegend
    Thursday, March 05, 2015
    Our group is interested in regulatory Th3 cells that express membrane bound TGF-beta. The only way to detect these cells is by surface staining of the TGF-beta complex by antibodies recognizing ... read more
  • Intracellular detection of IFN-gamma

    Intracellular detection of IFN-gamma

    BioLegend
    Monday, March 02, 2015
    Mouse transgenic OT-1 cells were stimulated with their cognate peptide: OVA for 3 days. On the last day, cells were re-stimulated with PMA/ionomycin and treated with Brefeldin A to allow accumulation ... read more
  • Anti-mouse CD8 for flow cytometry

    Anti-mouse CD8 for flow cytometry

    BioLegend
    Thursday, February 26, 2015
    I performed staining of mouse CD8 T cells, which were harvested from the splenocytes of a wild-type C57BL/6 mouse using bead based negative selection. The population of cells obtained was stained with... read more
  • Anti-Thy1.1 antibody for flow cytometry

    Anti-Thy1.1 antibody for flow cytometry

    BioLegend
    Thursday, February 26, 2015
    I transfected cells with a construct containing Thy1.1 as a reporter protein. Thy1.1 is a surface protein that is not usually expressed on mouse cells; so it is a great marker for detecting ... read more
  • Intracellular detection of TNF-alpha

    Intracellular detection of TNF-alpha

    BioLegend
    Wednesday, February 25, 2015
    This antibody was used for intracellular staining of TNFa on mouse CD8 T cells that were stimulated with OVA peptide. This seems to be a strong stimulation as most cells were positive for TNFa after 3... read more
  • PE/Cy7 anti-mouse CD25 Antibody for activation of T cell marker

    PE/Cy7 anti-mouse CD25 Antibody for activation of T cell marker

    BioLegend
    Wednesday, February 11, 2015
    Mouse total T cells were collected and activated by anti-CD3/CD28 Ab (hamster derived) in vitro (pre-coated with goat anti-hamster antibody) for 3 days and stained with this antibody and CD4 antibody.... read more
  • PE/Cy7 anti-mouse CD8a Antibody for CD8 T cell staining

    PE/Cy7 anti-mouse CD8a Antibody for CD8 T cell staining

    BioLegend
    Wednesday, February 11, 2015
    Naive spleen cells were collected from a mouse and stained with this antibody. During our experiments (including those run using this antibody), we used a number of different antibodies to look at ... read more
  • FITC anti-mouse F4/80 Antibody for macrophage staining

    FITC anti-mouse F4/80 Antibody for macrophage staining

    BioLegend
    Tuesday, February 10, 2015
    Mouse bone marrow was isolated and cultured in vitro with GM-CSF for 8 days, and stained with CD11b, Gr-1 and this antibody. read more
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