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  • PEI - An Inexpensive Transfection Reagent

    PEI - An Inexpensive Transfection Reagent

    Tuesday, March 25, 2014
    My research involves making lentiviral vectors pseudotyped with vesicular stomatitis virus envelop glycoprotein (VSV-G). In this protocol, I transfect HEK-293T cells with 3 plasmids; one for ... read more
  • Excellent Antibody for T-Cell Activation

    Excellent Antibody for T-Cell Activation

    eBioscience
    Tuesday, March 11, 2014
    My research focuses on T cell based immunotherapy. For this, I regularly activate and expand primary T cells from PBMCs. Anti-CD3 antibody is a very potent tool to activate T cells (in conjunction ... read more
  • A Good Anti-Mouse CD3e Antibody

    A Good Anti-Mouse CD3e Antibody

    eBioscience
    Tuesday, October 01, 2013
    We were trying to identify different subsets of T cell memory in mice. The first step in the staining is to gate on CD3+ cells among the lymphocytic gate on mouse splenocytes. We did this using this ... read more
  • Bright and good separation

    Bright and good separation

    eBioscience
    Thursday, September 05, 2013
    We measure Sca-1 in mice. I use this antibody in my multi-color flow-cytometry panel. Anti-mouse Sca-1 eF605NC works great at the recommended concentration for cells from the aorta. The IgG is low ... read more
  • Anti-Baculovirus Envelope gp-64 from eBioscience

    Anti-Baculovirus Envelope Antibody Used in Flow Cytometry

    eBioscience
    Wednesday, May 29, 2013
    This antibody is used for the detection of the baculovirus envelope protein gp-64. GP-64 protein is upregulated on the surface of insect cells shortly after transfection with baculovirus. Insect cells... read more
  • PolyJet DNA In Vitro Transfection Reagent from SignaGen Laboratories

    PolyJet DNA In Vitro Transfection Reagent from SignaGen Laboratories

    Friday, July 27, 2012
    PolyJet works very well, especially in HEK293 series cells, such as HEK293, HEK293T, HEK293FT. Compared to other transfection reagents, it is much less expensive but shows same efficiency of ... read more
  • Human IFN-alpha Platinum ELISA Kit from eBioscience

    Human IFN-alpha Platinum ELISA Kit from eBioscience

    eBioscience
    Wednesday, February 08, 2012
    Measuring the concentration of total interferon-alpha secreted by cells in peripheral blood samples, serum samples or even culture supernatants is not an easy task. There are 14 different isoforms of ... read more
  • Mouse IL-6 ELISA Ready-SET-Go! Kit (with Pre-Coated Plates) from eBioscience

    Mouse IL-6 ELISA Ready-SET-Go! Kit (with Pre-Coated Plates) from eBioscience

    eBioscience
    Monday, January 30, 2012
    The eBioscience IL-6 Ready-SET-Go! ELISA kit offers an easy and sensitive method to quantify IL-6 levels in biological samples. This is a ready-to-use kit which contains all of the required chemicals ... read more
  • Rat TNF alpha ELISA Ready-SET-Go! from eBioscience

    Rat TNF alpha ELISA Ready-SET-Go! from eBioscience

    eBioscience
    Monday, January 30, 2012
    The eBioscience TNF alpha Ready-SET-Go!® ELISA kit offers an easy and sensitive method to quantify TNF alpha in biological samples. Using this kit, we have estimated TNF alpha levels in the plasma ... read more
  • Human IL-8 ELISA Ready-SET-Go!  by eBioscience

    Human IL-8 ELISA Ready-SET-Go! by eBioscience

    eBioscience
    Wednesday, November 30, 2011
    The Human IL-8 ELISA, Ready-SET-Go!® Kit by eBioscience comes ready to use; with this kit you can run from 1 to 10 96-well ELISA plates. The plates are not included in the kit, but everything else ... read more
  • Targefect-RAW From Targeting Systems

    Targefect-RAW From Targeting Systems

    Targeting Systems
    Monday, June 08, 2009
    Targefect-RAW is a new reagent from Targeting Systems specifically designed for the transfection of the RAW 264.7 macrophage-like cell line. Although these cells are quite useful for studying innate ... read more
  • JetPEI™ Transfection Reagent From Polyplus Transfection

    JetPEI™ Transfection Reagent From Polyplus Transfection

    Wednesday, May 06, 2009
    Transfection refers to the introduction of foreign DNA into a eukaryotic cell, and can be achieved by simple uptake of a precipitate mixture of calcium phosphate and the DNA to be transfected. ... read more
  • Human or Mouse Regulatory T Cell Staining Kits From eBioscience

    Human or Mouse Regulatory T Cell Staining Kits From eBioscience

    eBioscience
    Thursday, October 25, 2007
    The regulatory T cell staining kits from eBioscience are designed to identify the subpopulation of T cells called T regulatory cells. These cells are characterized by expression of CD4 and high levels... read more
  • eBioscience's Human Regulatory T cell Staining Kit

    eBioscience's Human Regulatory T cell Staining Kit

    eBioscience
    Monday, April 24, 2006
    In our laboratory, we were looking for reliable reagents for Treg identification so that we could monitor these cells in a number of different experimental settings. So when we found the Human ... read more
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