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Antibody With Consistent, High Quality Performance in IF and Western Blotting
(1)
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MilliporeSigma
April 22, 2024
The study I conducted primarily involved investigating the role of Fragile X mental Retardation Protein (FMRP) in the post-transcriptional ...
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Reliable GFAP Antibody for Astrocytes in the Mouse Retina
(1)
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MilliporeSigma
April 19, 2024
This antibody was used in a study that investigated astrocyte pathology in the retina. This GFAP antibody has provided the best signal/...
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Satisfactory GLP-1 ELISA Kit From Millipore Sigma
(1)
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MilliporeSigma
February 22, 2024
We are interested in identifying orphan metabolites regulating mammalian physiology. We recently identified an exercise-inducible ...
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1,6-Hexanediol From Sigma Aldrich for Dissolving Condensates
(1)
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MilliporeSigma
February 13, 2024
I used 1,6-hexanediol to dissolve nuclear condensates, disrupting liquid-liquid phase separation and enabling the study of transcriptional ...
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Amazing Experience Using the Anti-RNA Polymerase II Antibody From Sigma-Aldrich for Labeling RNA Polymerase II
(1)
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MilliporeSigma
February 13, 2024
I study the transcriptional response in response to stress. I have used this antibody for labeling RNA polymerase II in cells.
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5-Iodo-2'-deoxyuridine From Sigma Aldrich is Ideal for Radiosensitization
(1)
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MilliporeSigma
February 12, 2024
5-Iodo-2'-deoxyuridine (5-IUdR; IdUrd) is a pyrimidine analog (halogenated thymidine) that has antiviral activity against vaccinia virus (...
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Best Antibody Option to Label Smooth Muscle
(1)
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MilliporeSigma
January 16, 2024
We study the CNS basement membrane and blood-brain barrier. We use this SMA antibody to label the Smooth muscles. This antibody works very ...
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PLGA From Sigma Aldrich is of High Quality
(1)
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MilliporeSigma
December 29, 2023
We used this polymer to obtain a cylindrical scaffold for drug release. It was easy to dissolve it in acetone and then with the ...
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Suitable Agarose Beads for Extracellular Vesicle Chromatography
(1)
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MilliporeSigma
December 28, 2023
Extracellular vesicles (EVs) were isolated from conditioned cell culture media from various cell types via chromatography in columns created...
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Cheap and Effective PEG From Sigma-Aldrich for Precipitation-Based Isolations
(1)
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MilliporeSigma
December 27, 2023
PEG buffer solution was prepared and used to isolate extracellular vesicles from conditioned cell culture media. Extracellular vesicles were...
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Simple Vacuum Filtration for Small Volumes
(1)
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MilliporeSigma
December 26, 2023
We perform a variety of cell cultures on primary human samples, which often require filter sterilization of small volumes of media or serum.
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IF Staining of L1 ORF1 Protein Expression in 4T1-tet L1(GLucAI) Cells
(1)
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MilliporeSigma
December 26, 2023
To assess the effects of L1 expression on the frequency of drug-resistant cell variants, we generated a stable population of 4T1 cells with ...
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Specific Neurofilament Light Chain Antibody
(1)
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MilliporeSigma
December 18, 2023
The specific goal of this experiment was to evaluate neurofilament light chain expression changes in the course of diseases. This antibody ...
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Probumin Bovine Serum Albumin From Millipore Works Very Well in IHC
(1)
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MilliporeSigma
November 28, 2023
There is no need to pay high prices for expensive counterparts. This albumin from Millipore works very well in all stages of ...
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Insulin Solution From Millipore Sigma Works Great
(1)
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MilliporeSigma
November 14, 2023
Insulin is a major glucose-lowering hormone, insufficiency of which causes hyperglycemia observed in type 2 diabetes. Insulin regulates the ...
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Penicillin-Streptomycin From Millipore Sigma is Ideal for HL-1 Cell Culture
(1)
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MilliporeSigma
November 14, 2023
Penicillin-Streptomycin is diluted 1:100 in Claycomb medium with 10% FBS, 2 mM L-glutamine, and 0.1 mM noradrenaline and used for HL-1 cell ...
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Cheap and Efficient DNA/Cell Death Staining With Propidium Iodide From Millipore Sigma
(1)
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MilliporeSigma
November 13, 2023
Propidium iodide from Merck is a decent dye with a high yield of signal for staining of DNA (cell cycle analysis) or cell death assessment. ...
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Great Anti-Histidine Tag Antibody From Millipore Sigma for Western Blot
(1)
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MilliporeSigma
November 09, 2023
We expressed receptor binding domain protein from SARS-CoV-2 in yeast cells and used this primary antibody to verify protein expression in ...
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Multiplex Kit for Hormones From MilliporeSigma
(1)
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MilliporeSigma
November 04, 2023
We are interested in measuring the effect of treatments in hormone production in reproductive gestational cells. This multiplex kit allows ...
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Amicon Ultra 2 mL From MilliporeSigma for Volumes Less Than 5 mL
(1)
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MilliporeSigma
November 02, 2023
Our volume outputs for some experiments are less than 3 mL. Therefore, for concentrating proteins or filtering out big molecules, we use an ...
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One of the Best Protease Inhibitors Out There From Millipore Sigma
(1)
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MilliporeSigma
November 02, 2023
cOmplete, Mini, EDTA-free Protease Inhibitor Tablets, inhibit a broad spectrum of serine and cysteine proteases. In contrast to other ...
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Superb Antifungal Reagent for Media From MilliporeSigma
(1)
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MilliporeSigma
October 24, 2023
Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. that is clinically used to treat systemic fungal infections. It has ...
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Vacuum Driven Filtration System From Millipore is Great for Filtering Small Cells
(1)
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MilliporeSigma
October 23, 2023
We use this for filtering small amounts of reagent or cell media. Works quickly and easily and helps prevent contamination.
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Selected Stable Gene Expression in HEK293 Cells With Disulfate Salt Solution From Merck
(1)
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MilliporeSigma
October 23, 2023
G418 stock was diluted to 250 mcg/ml in DMEM containing 10% FBS, 4 mM glutamine, 10 mM HEPES and puromycin (5 mcg/ml) and used to culture ...
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Filter Plate From Millipore Suitable for ELISPOT or MTS Assays
(1)
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MilliporeSigma
October 19, 2023
We use these plates to coat INF-y antibodies for ELISPOT or for cytotoxic assays. It is sturdy with deep wells for easy multi-channel ...
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Collagenase Type VIII From Sigma Aldrich for Tissue Digestion
(1)
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MilliporeSigma
October 12, 2023
My research area includes studying the role of inflammation in the development of colorectal cancer. I often use different types of ...
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Good Protein Extraction Kit From Sigma Aldrich Requiring Protocol Modifications
(1)
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MilliporeSigma
October 10, 2023
The ProteoExtract Native Membrane Protein Extraction Kit is a valuable resource for membrane protein isolation; however, some protocol ...
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Amicon Tube From Millipore for Exosome Isolation
(1)
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MilliporeSigma
October 05, 2023
Currently working with Neutrophil Extracellular Traps (NETs) stimulated by exosomes derived from amnion epithelial cells under oxidative ...
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Ideal Cell Freezing Container From Millipore
(1)
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MilliporeSigma
October 05, 2023
This device provides efficient freezing conditions for cell storage, it is a cooling container that gradually decreases temperature at a 1 °...
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Excellent Gram Stain Kit for Vaginal Smears
(1)
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MilliporeSigma
October 02, 2023
Gram Stain kit for diagnosing potential alterations in vaginal microbiota such as bacterial vaginosis and cytolytic vaginosis.
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A Rich Culture Media (BHI) to Grow Bacteria From Merck
(1)
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MilliporeSigma
September 14, 2023
I grow Enterococcus faecium bacteria and its mutant derivatives in Brain Heart Infusion (BHI) broth to isolate their cell walls and study ...
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High Quality Sulfatase From Millipore
(1)
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MilliporeSigma
September 14, 2023
We recently identified a phenyl sulfate metabolite regulated by fasting in mice. A key question is what enzymes are involved in the ...
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Perfect Cryopreservation Medium for Pre-Adipocytes From Sigma
(1)
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MilliporeSigma
September 12, 2023
We used this to prepare 10% Glycerol/90% FBS solution as a standard cryopreservation medium for pre-adipocytes and it works very well.
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Brilliant Actin Antibody For Western Blot From Millipore
(1)
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MilliporeSigma
September 11, 2023
This antibody was used in a study that investigated the induction of EMT in amnion epithelial cells (AEC) in response to inflammation and ...
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Brilliant Anti-phospho-TAB1 (Thr431) Antibody From Sigma Aldrich
(1)
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MilliporeSigma
September 09, 2023
This antibody was used in a study that investigated the induction of EMT in amnion epithelial cells (AEC) in response to inflammation and ...
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A Great Neuronal Cell Marker From Millipore Sigma
(1)
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MilliporeSigma
September 07, 2023
Our research focuses on neuro-vascular units in neurodegenerative diseases. The specific goal of this experiment was to quantify neuronal ...
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Reliable Anti-Flag Antibody From Millipore
(1)
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MilliporeSigma
August 23, 2023
To examine the protein level dynamics of the cell wall hydrolase, MepK during the cell cycle of E. coli, I conducted western blotting of ...
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Exceptionally Good IPTG to Induce Gene Expression in Bacteria
(1)
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MilliporeSigma
August 23, 2023
In a previous study conducted in our laboratory, we demonstrated that the presence of multiple copies of mepM, which encodes a paralog of ...
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Cephalexin, an Antimicrobial Agent by Sigma
(1)
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MilliporeSigma
August 20, 2023
I identified a putative cell wall hydrolase through a genetic screen. After creating a knockout of this gene, I aimed to determine its ...
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Lyophilized Micrococcus Lysodeikticus Cells From Millipore for Zymogram Analysis.
(1)
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MilliporeSigma
August 20, 2023
I have biochemically characterized a cell wall hydrolase, MepK. To assess its in vitro activity, I initially performed a Zymogram analysis ...
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Fantastic Monoclonal Anti-6X His Tag Antibody From Millipore
(1)
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MilliporeSigma
August 20, 2023
To study the functionality of a bacterial cell wall hydrolase (MepK), I have generated catalytically inactive variants of MepK in pTrc99a ...
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Fantastic enzyme to solubilize cell walls of bacteria
(1)
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MilliporeSigma
August 14, 2023
I work extensively with cell wall hydrolases (enzymes) of bacteria. To check the enzymatic activity, I have to solubilize the intact cell ...
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High Quality ɑ-Tubulin Detection in IF With Antibody From Sigma
(1)
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MilliporeSigma
August 09, 2023
This monoclonal Anti-ɑ-Tubulin antibody from Sigma/Merck, is the best option we found for the detection of Tubulin for IF stainings so ...
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Stable and Efficient Proteasome Inhibitor From Sigma Aldrich
(1)
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MilliporeSigma
August 09, 2023
The ubiquitin-proteasome system is the main protein degradation machinery in cells. To study the effect of proteasome inhibition on the ...
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MTT Assay for Cell Viability
(1)
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MilliporeSigma
August 04, 2023
I used the MTT assay as a simple, rapid, and reliable indicator of the cell viability of cultured cells under different treatment conditions...
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Stable and Effective CCCP From Sigma
(1)
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MilliporeSigma
August 04, 2023
CCCP is a potent uncoupler of oxidative phosphorylation and disrupts the mitochondrial membrane potential. We used CCCP as a positive ...
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Best Anti-α-Tubulin Antibody to Use in Western Blotting From MilliporeSigma
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MilliporeSigma
July 30, 2023
In my research, I aim to unravel the NFAT5 signaling mechanism by employing various protein biochemistry techniques, including protein ...
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Amicon® Ultra-15 Centrifugal Filters for Concentrating Proteins From Millipore
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MilliporeSigma
July 29, 2023
Amicon® Ultra-15 Centrifugal Filters are essential for concentrating proteins in biological research and biotechnological applications. ...
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TLC Silica Gel 60 Plates for Separation of Lipids
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MilliporeSigma, Merck
July 29, 2023
TLC Silica gel 60 plates are utilized for separating lipids from cell membrane extracts, enabling the identification and quantification of ...
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Effective Ultrafilter for Extracellular Vesicle/Exosome Concentration and Staining
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MilliporeSigma
July 28, 2023
Extracellular vesicles (EVs) isolated from cell culture media and resuspended in buffer were concentrated down to small volumes using 0.5ml ...
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