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  • Recombinanat Protein A-Sepharose 4B from Life Technologies

    Protein A Sepharose for Isolation of Chimeric Proteins

    Life Technologies
    Thursday, April 25, 2013
    Protein A binds the Fc region of immunoglobulins through interaction with the heavy chains. Beads coated with protein A can therefore be used to isolate chimeric recombinant proteins that have been ... read more
  • ANTI-FLAG® M2 Affinity Gel from Sigma-Aldrich

    ANTI-FLAG® M2 Affinity Gel from Sigma-Aldrich

    Monday, March 11, 2013
    We had a system with cells stably overexpressing FLAG-tagged RBPs. By transfecting the small RNA duplexes and immunoprecipitating (IP’ing) the FLAG-tagged RBPs, we could then perform a Northern blot ... read more
  • Mouse T Cell Enrichment Columns from R&D Systems

    Mouse T Cell Enrichment Columns from R&D Systems

    Tuesday, June 26, 2012
    The mouse T cell enrichment kit can be used for the purification of T cells from mouse splenocyte samples. Provided with the kit are the columns as well as the column wash buffer. The kit works via ... read more
  • CD11c Microbeads, Mouse Miltenyi Biotech

    CD11c Microbeads, Mouse Miltenyi Biotech

    Monday, June 11, 2012
    To isolate dendritic cells using the CD11c Microbeads is quite simple. Essentially, one must first obtain of a single cell suspension and to that suspension you will have to apply an appropriate ... read more
  • Co-expression with pETDuet-1 (Duet Expression System) From Novagen

    Co-expression with pETDuet-1 (Duet Expression System) From Novagen

    Novagen
    Monday, January 18, 2010
    Co-expression of several target genes in E. coli is advantageous for studying protein complexes; the expression of protein complexes can also sometimes help to increase the solubility of expressed ... read more
  • Overnight Express™ Autoinduction System From Novagen

    Overnight Express™ Autoinduction System From Novagen

    Novagen
    Tuesday, December 23, 2008
    Expression of recombinant protein in E. coli can be an efficient and useful technique to produce large quantities of protein quickly. The same plasmid that can be used in small-scale productions to ... read more
  • GeneJuice® Transfection Reagent From Novagen

    GeneJuice® Transfection Reagent From Novagen

    Novagen
    Thursday, February 21, 2008
    Transfection and expression of altered genes into cell lines are indispensable parts of genetic studies. There are many different methods for introducing genetic material into eukaryotic cells, ... read more
  • Rosetta-gami B From Novagen

    Rosetta-gami B From Novagen

    Novagen
    Monday, October 15, 2007
    Protein expression in bacteria is a widely used tool to produce important materials for research. The bacterial strain Rosetta-gami B from Novagen combines a number of useful features and mutations, ... read more
  • Rosetta-gami ™ B Host Strains From Novagen

    Rosetta-gami ™ B Host Strains From Novagen

    Novagen
    Friday, September 14, 2007
    To date, engineered E.coli has been used extensively as the cellular host for foreign protein expression due to its rapid growth rate, capacity for continuous fermentation, and relatively low cost. ... read more
  • Ni-NTA Agarose From Qiagen

    Ni-NTA Agarose From Qiagen

    Thursday, September 06, 2007
    The Nickel-Nitrilo triacetic acid (Ni-NTA) coupled to Sepharose CL-6B from Qiagen offers a quick and efficient method of protein purification from small and large scale preparations obtained through ... read more
  • SpinPrep™ PCR Clean-up Kit From Novagen

    SpinPrep™ PCR Clean-up Kit From Novagen

    Novagen
    Monday, September 18, 2006
    PCR is a routine technique in any molecular biology laboratory. A rapid method for purification of PCR product is, therefore, greatly appreciated in such laboratories. Novagen’s SpinPrep™ PCR Clean-up... read more
  • Novagen's D-Tube Dialyzer Kit

    Novagen's D-Tube Dialyzer Kit

    Novagen
    Tuesday, November 08, 2005
    Protein and nucleic bands can be extracted from either polyacrylamide or agarose gels by electro-elution of the molecules. To facilitate this method, Novagen (EMD Biosciences) offers the D-Tube ... read more
  • Novagen’s Factor Xa Cleavage Capture Kit

    Novagen’s Factor Xa Cleavage Capture Kit

    Novagen
    Tuesday, August 31, 2004
    The expression of recombinant proteins as translational fusions is commonly employed to enhance stability, increase solubility and facilitate purification of the desired protein... read more
  • Novagen's BugBuster GST-Bind Kit

    Novagen's BugBuster GST-Bind Kit

    Novagen
    Wednesday, November 19, 2003
    Recently, I needed to express and purify GST-fusion proteins for antibody production. Since neither I nor anyone else in the lab had done any protein expression work before, I wanted a kit that would... read more
  • Novagen's KOD DNA Polymerase

    Novagen's KOD DNA Polymerase

    Novagen
    Wednesday, June 05, 2002
    Novagen has introduced a fast and accurate recombinant form of DNA polymerase from the thermophile Thermococcus kodakaraensis. KOD HiFi DNA polymerase has a 3’ to 5’ exonuclease dependent proofreading... read more
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