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Protein A Sepharose for Isolation of Chimeric Proteins
Thursday, April 25, 2013
Protein A binds the Fc region of immunoglobulins through interaction with the heavy chains. Beads coated with protein A can therefore be used to isolate chimeric recombinant proteins that have been ...
ANTI-FLAG® M2 Affinity Gel from Sigma-Aldrich
Monday, March 11, 2013
We had a system with cells stably overexpressing FLAG-tagged RBPs. By transfecting the small RNA duplexes and immunoprecipitating (IP’ing) the FLAG-tagged RBPs, we could then perform a Northern blot ...
Mouse T Cell Enrichment Columns from R&D Systems
Tuesday, June 26, 2012
The mouse T cell enrichment kit can be used for the purification of T cells from mouse splenocyte samples. Provided with the kit are the columns as well as the column wash buffer. The kit works via ...
CD11c Microbeads, Mouse Miltenyi Biotech
Monday, June 11, 2012
To isolate dendritic cells using the CD11c Microbeads is quite simple. Essentially, one must first obtain of a single cell suspension and to that suspension you will have to apply an appropriate ...
Co-expression with pETDuet-1 (Duet Expression System) From Novagen
Monday, January 18, 2010
Co-expression of several target genes in
is advantageous for studying protein complexes; the expression of protein complexes can also sometimes help to increase the solubility of expressed ...
Overnight Express™ Autoinduction System From Novagen
Tuesday, December 23, 2008
Expression of recombinant protein in E. coli can be an efficient and useful technique to produce large quantities of protein quickly. The same plasmid that can be used in small-scale productions to ...
GeneJuice® Transfection Reagent From Novagen
Thursday, February 21, 2008
Transfection and expression of altered genes into cell lines are indispensable parts of genetic studies. There are many different methods for introducing genetic material into eukaryotic cells, ...
Rosetta-gami B From Novagen
Monday, October 15, 2007
Protein expression in bacteria is a widely used tool to produce important materials for research. The bacterial strain Rosetta-gami B from Novagen combines a number of useful features and mutations, ...
Rosetta-gami ™ B Host Strains From Novagen
Friday, September 14, 2007
To date, engineered
has been used extensively as the cellular host for foreign protein expression due to its rapid growth rate, capacity for continuous fermentation, and relatively low cost. ...
Ni-NTA Agarose From Qiagen
Thursday, September 06, 2007
The Nickel-Nitrilo triacetic acid (Ni-NTA) coupled to Sepharose CL-6B from Qiagen offers a quick and efficient method of protein purification from small and large scale preparations obtained through ...
SpinPrep™ PCR Clean-up Kit From Novagen
Monday, September 18, 2006
PCR is a routine technique in any molecular biology laboratory. A rapid method for purification of PCR product is, therefore, greatly appreciated in such laboratories. Novagen’s SpinPrep™ PCR Clean-up...
Novagen's D-Tube Dialyzer Kit
Tuesday, November 08, 2005
Protein and nucleic bands can be extracted from either polyacrylamide or agarose gels by electro-elution of the molecules. To facilitate this method, Novagen (EMD Biosciences) offers the D-Tube ...
Novagen’s Factor Xa Cleavage Capture Kit
Tuesday, August 31, 2004
The expression of recombinant proteins as translational fusions is commonly employed to enhance stability, increase solubility and facilitate purification of the desired protein...
Novagen's BugBuster GST-Bind Kit
Wednesday, November 19, 2003
Recently, I needed to express and purify GST-fusion proteins for antibody production. Since neither I nor anyone else in the lab had done any protein expression work before, I wanted a kit that would...
Novagen's KOD DNA Polymerase
Wednesday, June 05, 2002
Novagen has introduced a fast and accurate recombinant form of DNA polymerase from the thermophile
. KOD HiFi DNA polymerase has a 3’ to 5’ exonuclease dependent proofreading...
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Picture Books Aren’t Just Fun: Moms Are Exposing Toddlers To Rich Information About Animals
Wednesday, April 23, 2014
Children hear as much sophisticated information about animals when parents read picture book stories...
Risk Of Pregnancy Greater With Newer Method Of Female Sterilization
Wednesday, April 23, 2014
The risk of pregnancy among women using a newer method of planned sterilization called ...
Scientists Alter Fat Metabolism In Animals To Prevent Most Common Type Of Heart Disease
Wednesday, April 23, 2014
Working with mice and rabbits, Johns Hopkins scientists have found a way to block abnormal ...
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