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  • Effectene™ Transfection Reagent From Qiagen

    Effectene™ Transfection Reagent From Qiagen

    Wednesday, November 08, 2006
    Transfection reagents and protocols have become very widespread and utilized in many aspects of DNA research. There are many applications that require the introduction of vectors and expression ... read more
  • RNeasy Mini Kit From Qiagen

    RNeasy Mini Kit From Qiagen

    Tuesday, August 29, 2006
    Isolation of RNA from mammalian cells is usually straightforward; however; bacterial cells may pose additional problems. I have used Qiagen’s RNeasy Mini Kit extensively on mammalian cells with ... read more
  • RNeasy Midi Kit From Qiagen

    RNeasy Midi Kit From Qiagen

    Thursday, July 20, 2006
    Good RNA preparation is a prerequisite to performing several experiments including cDNA synthesis, real-time quantitative PCR (qPCR) and promoter mapping. I have been using RNeasy Midi Kit for quite ... read more
  • QIAGEN's QIAprep Spin Miniprep Kit

    QIAGEN's QIAprep Spin Miniprep Kit

    Tuesday, April 18, 2006
    This kit from QIAGEN allows you to go from bacterial culture to reasonably pure plasmid in about 20 minutes using a simple lysis, followed by elution from a supplied bind-and-release spin column. read more
  • Qiagen's QIAprep Spin Miniprep Kit

    Qiagen's QIAprep Spin Miniprep Kit

    Thursday, February 09, 2006
    Submitted by:
    Boyd Scott
    Senior Research Scientist/Vitae Pharmaceuticals

    Qiagen’s QIAprep Miniprep Kit is based on alkaline lysis of bacteria followed by adsorption onto a silica-gel-membrane in the ... read more
  • QIAGEN’s QIAprep Miniprep Kit

    QIAGEN’s QIAprep Miniprep Kit

    Wednesday, November 30, 2005
    Pure plasmid DNA is a critical component to success in any molecular biology lab as the majority of follow-up applications, from sequencing to transfection, relies on uncontaminated DNA. The Qiagen ... read more
  • QIAGEN's Endofree Plasmid Maxi Kit

    QIAGEN's Endofree Plasmid Maxi Kit

    Thursday, November 17, 2005
    Our lab works on the molecular manipulation of unicellular organisms and cell lines. One major aspect of our research is the transfection of plasmid DNA into these cells via injection or ... read more
  • Qiagen HiSpeed Midi Plasmid DNA Purification Kit

    Qiagen HiSpeed Midi Plasmid DNA Purification Kit

    Tuesday, October 11, 2005
    All of Qiagen’s plasmid purification kits are based on a modified alkaline lysis protocol, followed by the binding of DNA to an anion-exchange resin at low salt and pH conditions. Impurities are ... read more
  • Qiagen’s QIAprep Spin Miniprep kit

    Qiagen’s QIAprep Spin Miniprep kit

    Tuesday, August 09, 2005
    Today, it seems like every company on the market sells a plasmid miniprep kit, thus, researchers have several to choose from. Our lab is currently using Qiagen’s QIAprep miniprep kit as our miniprep ... read more
  • Qiagen's QIAquick PCR Purification Kit

    Qiagen's QIAquick PCR Purification Kit

    Wednesday, July 20, 2005
    Every molecular biologist is concerned about the purity of their DNA samples. Whether performing RT-PCR, nested PCR, sequencing or fragment analysis, a critical step for a successful outcome is the ... read more
  • Qiagen’s TransMessenger Transfection Reagent

    Qiagen’s TransMessenger Transfection Reagent

    Wednesday, February 26, 2003
    The TransMessenger Transfection Reagent from Qiagen is designed for the efficient transfection of eukaryotic cells with RNA (including siRNA). The reagent works using principles similar to the ... read more
  • Qiagen's Genomic Tip G/100

    Qiagen's Genomic Tip G/100

    Thursday, May 16, 2002
    The G/100 columns isolate genomic DNA fragments ranging from 20 to 150 kilobase pairs (kbp) in size directly from whole blood, cultured cells, tissues, mouse tails, yeast, and both Gram-negative and ... read more
  • Qiagen’s RNeasy Mini Kit

    Qiagen’s RNeasy Mini Kit

    Thursday, March 07, 2002
    The RNeasy kit can purify total RNA from animal cells or tissues, bacteria and yeast and another similar kit is available for the extraction of total RNA from plants and filamentous fungi, the RNeasy ... read more
  • QIAGEN’s OmniScript Reverse Transcriptase Kit

    QIAGEN’s OmniScript Reverse Transcriptase Kit

    Wednesday, August 08, 2001
    Some time ago I came across Qiagen’s OmniScript Reverse Transcriptase system to prepare CyDye labeled cDNA from total RNA (or mRNA) for microarray hybridization. Since then, I have used this ... read more
  • Qiagen HiSpeed Plasmid Purification Kit

    Qiagen HiSpeed Plasmid Purification Kit

    Wednesday, May 30, 2001
    Qiagen produces an excellent range of products for the isolation and purification of nucleic acids in various contexts. Recently, they have added to this line-up with the release of the HiSpeed ... read more
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