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  • SuperSignal West Pico Chemiluminescent Substrate From Pierce

    SuperSignal West Pico Chemiluminescent Substrate From Pierce

    Thermo Scientific Pierce Protein Research Products
    Friday, February 02, 2007
    Western blot is a technique that was introduced by Towbin, et al. in 1979; today it is routinely used by many laboratories for protein analysis. The whole procedure involves many steps that must be ... read more
  • BCA Protein Assay Kit From Pierce

    BCA Protein Assay Kit From Pierce

    Thermo Scientific Pierce Protein Research Products
    Monday, January 08, 2007
    Standard protocols for protein estimation, for example, Lowry and Bradford's protocols, are available in any standard protein protocol book. With the number of buffers and chemicals used for the ... read more
  • LightShift EMSA Kit From Pierce

    LightShift EMSA Kit From Pierce

    Thermo Scientific Pierce Protein Research Products
    Monday, January 08, 2007
    Our lab uses this kit to study the DNA-binding properties of different transcription factors involved in the regulation of aging using worms as a model system. We decided to employ the Pierce ... read more
  • Superscript™ III Reverse Transcriptase From Invitrogen

    Superscript™ III Reverse Transcriptase From Invitrogen

    Life Technologies
    Monday, January 08, 2007
    Invitrogen’s Superscript™ III reverse transcriptase (RT) provides a fast, easy-to-use system for the generation of first strand cDNA (which can be used in subsequent PCR reactions) from mRNA. Gene-... read more
  • Chemiluminescent Nucleic Acid Detection Module From Pierce

    Chemiluminescent Nucleic Acid Detection Module From Pierce

    Thermo Scientific Pierce Protein Research Products
    Tuesday, January 02, 2007
    We use the Pierce Chemiluminescent Nucleic Acid Detection Module to detect biotin-labeled nucleic acids containing the putative binding site of our protein of interest, Heat Shock Factor (HSF), in ... read more
  • ECL Western Blotting Substrate From Pierce

    ECL Western Blotting Substrate From Pierce

    Thermo Scientific Pierce Protein Research Products
    Wednesday, December 13, 2006
    Our laboratory has come to rely on the Pierce ECL Western Blotting Substrate for detection of target proteins on immunoblots for Western blot analysis. This substrate is very sensitive; it is an ... read more
  • EZ Biotin Quantitation Kit From Pierce

    EZ Biotin Quantitation Kit From Pierce

    Thermo Scientific Pierce Protein Research Products
    Friday, December 08, 2006
    Biotinylation of proteins is one of the most common formats for creating biomolecules for use as detection reagents. Because it is such a widely used technique, there are a large number of ... read more
  • Seize X Mammalian Immunoprecipitation Kit From Pierce

    Seize X Mammalian Immunoprecipitation Kit From Pierce

    Thermo Scientific Pierce Protein Research Products
    Tuesday, November 21, 2006
    This kit contains immobilized Protein G Beads, Binding/Wash Buffer, Elution Buffer (gentle elution buffer of high ionic strength is available separately), Handee Spin Cup Columns and microfuge tubes, ... read more
  • Compat-Able Protein Assay Kit From Pierce

    Compat-Able Protein Assay Kit From Pierce

    Thermo Scientific Pierce Protein Research Products
    Thursday, November 02, 2006
    I typically use the BCA assay method to determine the concentration of protein samples. The protein that I am currently studying tends to aggregate and, after following an approach described in a ... read more
  • CarboLink Coupling Gel From Pierce

    CarboLink Coupling Gel From Pierce

    Thermo Scientific Pierce Protein Research Products
    Wednesday, October 25, 2006
    Pierce’s CarboLink Coupling Gel is an affinity support for immobilizing glycoproteins through oxidized sugar groups. It is ideal for immobilizing polyclonal antibodies, which contain abundant ... read more
  • Coomassie (Bradford) Protein Assay Kit From Pierce

    Coomassie (Bradford) Protein Assay Kit From Pierce

    Thermo Scientific Pierce Protein Research Products
    Thursday, October 19, 2006
    The Pierce Coomassie Protein Assay Kit is used in our laboratory for quick quantification of total protein for prepared extracts from C. elegans. This kit is sufficient for 630 test tube assays or ... read more
  • Restore™ Western Blot Stripping Buffer From Pierce

    Restore™ Western Blot Stripping Buffer From Pierce

    Thermo Scientific Pierce Protein Research Products
    Thursday, October 12, 2006
    For the last two years, I have been using Pierce’s Restore™ Western Blot Stripping Buffer for serial blotting of membranes containing samples of non small cell lung cancer cell line lysates. The use ... read more
  • Pro-Matrix™ Protein Refolding Kit From Pierce

    Pro-Matrix™ Protein Refolding Kit From Pierce

    Thermo Scientific Pierce Protein Research Products
    Wednesday, September 06, 2006
    The expression of recombinant proteins in bacteria occasionally results in the improper folding of the expressed proteins. Since proper folding is required not only for a protein’s activity but also ... read more
  • Pierce's SeizeX Immunoprecipitation Kit

    Pierce's SeizeX Immunoprecipitation Kit

    Thermo Scientific Pierce Protein Research Products
    Wednesday, April 26, 2006
    The Seize X Immunoprecipitation Kit offers an excellent solution for immunopurifying proteins or complexes without co-elution of the capturing antibody... read more
  • Pierce’s Lightshift Chemiluminescent EMSA Kit

    Pierce’s Lightshift Chemiluminescent EMSA Kit

    Thermo Scientific Pierce Protein Research Products
    Tuesday, February 21, 2006
    Submitted by:
    Jeffrey Perry
    University of Michigan

    Traditionally, electromobility gel shift assays (EMSA) using radioactive DNA probes were the most common method for determining the activity of a ... read more
  • Pierce’s Supersignal West Pico Horseradish Peroxidase (HRP) Detection Kit

    Pierce’s Supersignal West Pico Horseradish Peroxidase (HRP) Detection Kit

    Thermo Scientific Pierce Protein Research Products
    Tuesday, January 10, 2006
    The Pierce SuperSignal Substrate system is one of these standards that has stood the test of time. In my lab, this kit has been used for over 10 years for a variety of detection applications ... read more
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