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Oligos for CRISPR Technology From IDT
(1)
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Integrated DNA Technologies
September 13, 2023
I am targeting actin-related genes for knockdown. Actin-related genes help in actin cytoskeleton nucleation and branching. I ordered oligos ...
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Genome Editing Efficiency Determination by T7 Endonuclease I (T7EI) Mismatch Cleavage Assay
(1)
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Integrated DNA Technologies
July 25, 2023
I routinely use CRISPR/Cas9-based methodology to generate gene knockouts in cell lines. As a first step, I do pooled gene knockout using the...
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HDR Enhancer V2 to Improve Endogenous Editing Efficiency
(1)
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Integrated DNA Technologies
July 21, 2023
I use precise genome editing to generate endogenous mutations in the components of the WNT pathway. My previous attempts using the CRISPR/...
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Good for Improving Delivery of Cas9/RNP by Electroporation
(1)
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Integrated DNA Technologies
March 23, 2023
Cas9 and gRNA forms RNP with addition of enhancer. When transfected in 3T3 cells, enhancer increased the knockout efficiency by 2 fold.
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Good for Selecting Cas9-RNP Targeted Cell
(1)
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Integrated DNA Technologies
March 15, 2023
tracrRNA, ATTO™ 550 was used to form gRNA and RNP complex with Cas9 protein. After 24 hours of transfection. RNP-targeted cells were sorted ...
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Good Signal for Selecting Positive Cells
(1)
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Integrated DNA Technologies
March 07, 2023
Cas9-GFP was used for form RNP complex to knockout gene in vitro. The results showed that the GFP signal is relative strong and easy to ...
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tracrRNA ATTO550 Fluorescent by IDT
(1)
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Integrated DNA Technologies
January 04, 2023
I am performing CRISPR-Cas9 experiment on HEK and B cells. I always use Integrated DNA Technology's product for electroporation of RNP ...
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tracrRNA for RNP Complex by IDT
(1)
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Integrated DNA Technologies
January 03, 2023
I am performing CRISPR-Cas9 experiments on B cells and HEK to edit genes making membrane proteins. To do so, I am using a protocol for ...
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Electroporation Enhancer by IDT
(1)
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Integrated DNA Technologies
December 29, 2022
I am performing CRISPR-Cas9 experiments on B cells and I have compared KO efficiency with and without this reagent that is supposed to ...
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Cas9 Nuclease by IDT
(1)
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Integrated DNA Technologies
December 29, 2022
I am performing CRISPR-Cas9 experiment on B cells and HEK cells. I am using Cas9 protein from IDT which is part of my protocol for RNP-...
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Primer Design for Gene of Interest
(1)
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Integrated DNA Technologies
January 22, 2019
The aim of the task was to design multiple primers for investigating the expression of specific genes.
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Integrated DNA Technologies For Fast And Efficient Primer Synthesis And Delivery
(1)
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Integrated DNA Technologies
March 30, 2017
We regularly use IDT to provide high quality custom oligos for PCR experiments
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Oligo Synthesis Delivered Quickly
(1)
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Integrated DNA Technologies
February 24, 2017
I've tried a couple oligo synthesis providers, but have had the best experience with IDT. Even late afternoon orders are usually delivered ...
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Salmonella Enterica Gene Expression By Multiplexed QPCR Assay
(1)
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Integrated DNA Technologies
July 19, 2016
I have used Integrated DNA technologies Prime Time Gene expression master mix in a multiplexed qPCR assay to measure expression of key genes...
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Probe-Based qPCR Assay for Relative Gene Expression Analysis
(1)
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Integrated DNA Technologies
March 22, 2016
Expression of genes involved in bile stress in E. coli were analysed by performing probe based multiplexed qPCR assays using probes and ...
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Great Oligonuclutides at a Good Price
(1)
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Integrated DNA Technologies
February 17, 2016
I have had a good experience ordering primers and custom oligonucleuotides from IDT. They have always given me high-quality primers, shipped...
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Excellent Custom Oligonucleotide Service
(1)
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Integrated DNA Technologies
October 20, 2015
We study changes in gene expression using qPCR. Our primers are from IDT DNA because they have a clean website interface, useful tools for ...
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IDT has the best DNA/RNA oligos
(1)
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Integrated DNA Technologies
May 29, 2015
We have been ordering oligos from IDT for a long time now. 99% of the oligos work very well.
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qRT-PCR Primers
(1)
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Integrated DNA Technologies
August 08, 2014
It is a great pleasure to write review on Integrated DNA technologies which provides qRT-PCR primers. We were screening expression of 500 ...
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Custom Oligonucleotides From Integrated DNA Technologies
Integrated DNA Technologies
May 21, 2008
Integrated DNA Technologies (IDT) synthesizes custom DNA and RNA sequences to order. As a molecular biologist, I buy a lot of primers. ...
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