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  • HSP-60 antibody staining on stem cells

    HSP-60 antibody staining on stem cells

    Monday, November 17, 2014
    The antibody was used to detect the effect of environmental toxicants on mitochondrial unfolded protein response. Specifically, this product was used to visualize heat shock protein -60 in ... read more
  • Mouse specific cleaved PARP (cell death marker)

    Mouse specific cleaved PARP (cell death marker)

    Wednesday, November 12, 2014
    Programed cell death (PCD) is a critical process that underlies normal tissues development and serves as a homeostatic mechanism to maintain cell populations. Importantly, dysregulation of PCD has ... read more
  • Great Antibody for FLAG immunoprecipitations

    Great Antibody for FLAG immunoprecipitations

    Tuesday, November 11, 2014
    Our lab uses Anti-FLAG® M2 Magnetic Beads (produced in mouse, Sigma) for protein interaction co-immunoprecipitations. Invariably, some of the mouse antibody carries through with the eluted sample, ... read more
  • Western blotting via anti-Bcl-x

    Western blotting via anti-Bcl-x

    Monday, November 10, 2014
    Anti-Bcl-x protein expression was characterized via western blot by using purified rabbit Anti-Bcl-x (1:500) read more
  • Evaluating CD62E expression by IF

    Evaluating CD62E expression by IF

    Friday, October 31, 2014
    R and D systems mouse anti-human CD62E was tested on 293T human cells ectopically expressing human CD62E by immunoflourescence. Fixation and blocking steps were used that would impede the antibody ... read more
  • Sigma FLAG Antibody

    Sigma FLAG Antibody

    Wednesday, October 22, 2014
    We used this antibody for Western blotting of the FLAG-tagged proteins expressed in mammalian cell lines. Works well if stored properly. Regular freezing and thawing results in poor results. read more
  • KAT6A antibody evaluated for Western blot and pull down experiment

    KAT6A antibody evaluated for Western blot and pull down experiment

    Monday, October 13, 2014
    In my experiment we study protein-protein interaction between KAT6a and a KAT6a interacting protein. The antibody gave very nice pull-down but in western blot besides main band it showed several minor... read more
  • Very good antibody for detection of SHP2 by immunofluoresence

    Very good antibody for detection of SHP2 by immunofluoresence

    Wednesday, October 01, 2014
    The goal of this experiment was to determine the cellular localization of SHP2 in mouse embryonic fibroblasts (MEFs) read more
  • Use of Tendem Dye for Multicolor Flow Cytometry

    Use of Tendem Dye for Multicolor Flow Cytometry

    BioLegend
    Friday, August 15, 2014
    The aim of my study was to investigate CD4+ T cell proliferation. I harvest mouse splenocytes and use following antibodies from Biolegend to isolate CD4+ T cells and B cells: APC conjugated anti ... read more
  • Application of PE Conjugated Anti Human MDR1 Ab

    Application of PE Conjugated Anti Human MDR1 Ab

    BioLegend
    Thursday, August 07, 2014
    We are trying to look at the expression of MDR1 in different cell lines by FACS. This Ab works great, as it can be directly used to stain the tiny part of surface MDR1. read more
  • The Usage of PP2Ac ab

    The Usage of PP2Ac ab

    EMD Millipore
    Wednesday, August 06, 2014
    To detect the protein abundance of PP2Ac in arabidopsis with this PP2Ac antibody. This antibody uses the most conserved sequence of PP2Ac found in most organisms. The detection range could be down to ... read more
  • Intracellular IFN-g Staining for Mouse Cells by Flow Cytometry

    Intracellular IFN-g Staining for Mouse Cells by Flow Cytometry

    BioLegend
    Wednesday, August 06, 2014
    Mouse splenocytes were activated by anti-CD3/anti-CD28 for 96 hours, in polarizing conditions towards Tc1/Th1 or Tc17/Th17, followed by restimulation with PMA/Ionomycin in presence of brefeldin-A (... read more
  • EMT Analysis: Lef1 Detection by WB

    EMT Analysis: Lef1 Detection by WB

    Cell Signaling Technology
    Wednesday, August 06, 2014
    Epithelial to Mesenchimal Transcription (EMT) is a cellular process in which cells transform their phenotype into a more fibroblast-like one. Snail1 protein has been described as the critical player ... read more
  • NFkB - p65 Detection by WB

    NFkB - p65 Detection by WB

    Santa Cruz Biotechnology, Inc.
    Wednesday, August 06, 2014
    NfKB pathway is one most important transcriptional regulator in many cellular and physiological processes. Destabilization of NfKB protein (or patahway) ocurrs in many cancer types, so a good ... read more
  • An Antibody that Serves the Purpose, Wonderfully

    An Antibody that Serves the Purpose, Wonderfully

    Novus Biologicals
    Tuesday, July 29, 2014
    I am wanted to check the level of MLL1 in HL-60 and HEk-293T cells before I could silence the gene and study its effects. read more
  • Very Good Antibody for Actin Western Blot

    Very Good Antibody for Actin Western Blot

    Novus Biologicals
    Monday, July 28, 2014
    I use this antibody as a loading control for the western blots. read more
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