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  • hnHotStar Taq DNA Polymerase From Qiagen

    hnHotStar Taq DNA Polymerase From Qiagen

    Wednesday, October 27, 2010
    PCR is now a standard practice for all labs which are into molecular based examination. To get reproducible results, a lab should use well-standardized products which are sensitive and... read more
  • Mouse IL-1beta ELISA MAX Standard by BioLegend

    Mouse IL-1beta ELISA MAX Standard by BioLegend

    BioLegend
    Thursday, January 26, 2012
    Mouse IL-1beta ELISA MAX™ Standard Kit by BioLegend comes with many of the supplies necessary to complete ELISAs using 5, 10, or 20 96-well plates. The plates are not included in the kit; the kit ... read more
  • PE/Cy5 Anti-Human CD56 (NCAM) Antibody: Good Value

    PE/Cy5 Anti-Human CD56 (NCAM) Antibody: Good Value

    BioLegend
    Monday, August 05, 2013
    We work with human NK cells, performing a lot of flow cytometry assays. The BioLegend PE/Cy5 anti-human CD56 (NCAM) antibody has always given us good, consistent staining. The antibody is also ... read more
  • High Quality CD3 Antibody

    High Quality CD3 Antibody

    BioLegend
    Monday, August 19, 2013
    In order to stain human lymphocytic sub-populations, it is often indispensable to use CD3. As a prevalent antigen, we chose a "dim" fluorophore in APC/Cy7. The results were very good, and this ... read more
  • PE/Cy7 Anti-Human CD4 Antibody: Strong Signal

    PE/Cy7 Anti-Human CD4 Antibody: Strong Signal

    BioLegend
    Monday, August 26, 2013
    Our studies concentrate on identifying T cell memory subsets. The initial step in the identification of these cells is staining for CD4 and CD8. We have been using a very good CD4/CD8 colour ... read more
  • Reliable FITC CD94 Antibody

    Reliable FITC CD94 Antibody

    BioLegend
    Thursday, August 22, 2013
    CD94 is highly expressed on CD56bright NK cells. We stain for CD94 regularly and need a good, reliable antibody. BioLegend's FITC anti-human CD94 Antibody fits the bill. read more
  • Bright Signal from BV711 Conjugated Anti-CD56 (NCAM)

    Bright Signal from BV711 Conjugated Anti-CD56 (NCAM)

    BioLegend
    Wednesday, July 31, 2013
    Our lab studies human NK cell immunotherapies. As such we frequently analyze NK cell phenotypic changes after immunotherapuetic regimens. read more
  • APC Anti-Human NKG2D Antibody: Highly Recommended

    APC Anti-Human NKG2D Antibody: Highly Recommended

    BioLegend
    Monday, September 09, 2013
    This antibody was used in a study that investigated the role of NKG2D receptor in the regulation of NK cell migration. This work has been published in European Journal of Immunology in 2012 (Serrano-... read more
  • PE anti-mouse Tim-1

    PE anti-mouse Tim-1

    BioLegend
    Wednesday, August 28, 2013
    Our lab studies murine T cells; specificalloy we are interested in isolating the Tim-1+ subpopulation. Because there is no commercially available kit for for TIM-1+ T cell isolation, we combine the PE... read more
  • Antibody of Choice for Anti-Mouse F4/80

    Antibody of Choice for Anti-Mouse F4/80

    BioLegend
    Wednesday, October 09, 2013
    I used this antibody to identify macrophages from Aorta and Kidney. For Aorta, I titrated the concentration to 3ul for 10^6 cells in 100ul and for Kidney I use around 5ul for 10^6 cells in 100ul. ... read more
  • Good Antibody at Affordable Price

    Good Antibody at Affordable Price

    BioLegend
    Tuesday, November 19, 2013
    Anti-mouse CD11b BV711 is a great antibody. Works good with cells from kidney. I selected this antibody for multicolor flow cytometry experiments to identify macrophages in kidney. Other markers we ... read more
  • Detecting KIR2DL1 and 2DS1

    Detecting KIR2DL1 and 2DS1

    BioLegend
    Monday, November 25, 2013
    Detecting KIRs (killer cell immunoglobulin-like receptors) on human natural killer cells is not always easy. We have found that some antibodies work better than others, despite the manufacturer's ... read more
  • Good Bcl-6 Antibody for Flow Cytometry

    Good Antibody for Flow Cytometry

    BioLegend
    Wednesday, November 27, 2013
    I was looking for a good BCL6 antibody for flow cytometry. After trying 5 different antibodies, I found this one to be very good and bright. I have used it for nearly a year. read more
  • PE-CD8a IF Staining

    PE-CD8a IF Staining

    BioLegend
    Tuesday, December 10, 2013
    Used Biolegend PE anti-mouse CD8a for IF staining of cyropreserved sections sections and it worked well to illuminate splenic CD8 T cells. read more
  • Alexafluor 647-Conjugated Anti-Human CD44 Antibody

    Alexafluor 647-Conjugated Anti-Human CD44 Antibody

    BioLegend
    Tuesday, December 10, 2013
    This antibody was used to sort for CD44-positive cells in primary human keratinocytes. I selected this antibody because it was conjugated and had been previously tested by the company. read more
  • Great PD-1 Antibody for Flow Cytometry

    Great PD-1 for Flow Cytometry

    BioLegend
    Wednesday, January 15, 2014
    I work in a murine system to characterize antiviral responses when the animals are exposed to exogenous immunological insults. I have used a two step PD-1 stain in the past in which we used ... read more
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