Product Reviews

Filter

Hide Filter
  • PEI - An Inexpensive Transfection Reagent

    PEI - An Inexpensive Transfection Reagent

    Tuesday, March 25, 2014
    My research involves making lentiviral vectors pseudotyped with vesicular stomatitis virus envelop glycoprotein (VSV-G). In this protocol, I transfect HEK-293T cells with 3 plasmids; one for ... read more
  • PrimeSTAR GXL DNA Polymerase Amplifies Genomic DNA from Crude C. elegans Extracts

    PrimeSTAR GXL DNA Polymerase

    Takara Bio
    Thursday, February 20, 2014
    For this project, my goal was to use a PCR-based strategy to genotype C. elegans strains in order to confirm the presence or absence of specific mutations. Because I was genotyping anywhere from 4-16... read more
  • SapphireAmp Fast PCR Master Mix

    SapphireAmp Fast PCR Master Mix

    Takara Bio
    Thursday, February 06, 2014
    This reagent is designed to rapidly set up a hot start PCR. This made it ideal for large scale genotyping to assess a single nucleotide polymorphism of interest. The reagents were successful in a ... read more
  • PolyJet DNA In Vitro Transfection Reagent from SignaGen Laboratories

    PolyJet DNA In Vitro Transfection Reagent from SignaGen Laboratories

    Friday, July 27, 2012
    PolyJet works very well, especially in HEK293 series cells, such as HEK293, HEK293T, HEK293FT. Compared to other transfection reagents, it is much less expensive but shows same efficiency of ... read more
  • PolyFect Transfection Reagent From Qiagen

    PolyFect Transfection Reagent From Qiagen

    Thursday, February 28, 2008
    In our experiment, I used Qiagen’s QIAEXII Gel Extraction Kit (Cat. No. 20021) to prepare the DNA prior to transfection so that it was clear of bacterial endotoxins. I used the “desalting protocol” ... read more
  • GeneJuice® Transfection Reagent From Novagen

    GeneJuice® Transfection Reagent From Novagen

    Novagen
    Thursday, February 21, 2008
    Transfection and expression of altered genes into cell lines are indispensable parts of genetic studies. There are many different methods for introducing genetic material into eukaryotic cells, ... read more
  • LA Taq™ With GC Buffer From Takara

    LA Taq™ With GC Buffer From Takara

    Takara Bio
    Friday, October 05, 2007
    TaKaRa LA (Long and accurate) Taq™ is a thermostable DNA polymerase having 3'-5' exonuclease activity (proof reading activity) that is capable of, under general PCR conditions, higher efficiency and ... read more
  • Lipofectamine™ 2000 From Invitrogen

    Lipofectamine™ 2000 From Invitrogen

    Life Technologies
    Tuesday, September 25, 2007
    A common method used in almost every lab these days is transient transfection of cell lines followed by some form of output (Western blot, reporter gene assays, etc.). In a majority of cases, one ... read more
  • PLUS™ Reagent From Invitrogen

    PLUS™ Reagent From Invitrogen

    Life Technologies
    Tuesday, September 25, 2007
    Efficient and effective transfection reagents are essential for protein expression in eukaryotic cells. Recent years have seen a rapid expansion in the number and type of transfection reagents ... read more
  • Lipofectamine™ LTX Transfection Reagent From Invitrogen

    Lipofectamine™ LTX Transfection Reagent From Invitrogen

    Life Technologies
    Tuesday, September 25, 2007
    Invitrogen have added a new product, Lipofectamine™ LTX, to their extensive range of transfection products. Following on from other products in the Lipofectamine range, Lipofectamine™ LTX is a lipid ... read more
  • Lipofectamine™ 2000 Transfection Reagent From Invitrogen

    Lipofectamine™ 2000 Transfection Reagent From Invitrogen

    Life Technologies
    Tuesday, March 27, 2007
    Lipofectamine™ 2000 Reagent is a lipid-derived reagent designed to deliver exogenic nucleic acids (DNA and/or RNA) into eukaryotic cells, either adherent or in suspension. Thee two most important ... read more
  • Chaperone Plasmid Set From Takara Mirus Bio

    Chaperone Plasmid Set From Takara Mirus Bio

    Takara Bio
    Friday, January 26, 2007
    As with many other laboratories, we rely heavily on large-scale recombinant protein expression in our effort to conduct genetic and proteomic analysis of parasite-host cell interactions. There are ... read more
  • DNA Ligation Kit, Version 1 From Takara

    DNA Ligation Kit, Version 1 From Takara

    Takara Bio
    Monday, January 08, 2007
    How did I get to know the DNA Ligation Kit from Takara? Around 2 years ago, I was trying to do a cloning and I was stuck at the ligation step. At first, I was using the regular T4 DNA ligase and ... read more
  • SYBR® Premix Ex Taq™ (Perfect Real Time) From Takara Mirus Bio

    SYBR® Premix Ex Taq™ (Perfect Real Time) From Takara Mirus Bio

    Takara Bio
    Monday, January 08, 2007
    SYBR® Premix Ex Taq™ (Perfect Real Time) is a 2X concentration of premixed reagent which includes Takara’s Ex Taq™ HS and SYBR® Green I. The Ex Taq™ HS is an enzyme for hot start PCR... read more
  • Lipofectamine™ 2000 from Invitrogen

    Lipofectamine™ 2000 from Invitrogen

    Life Technologies
    Friday, December 01, 2006
    Transfection reagents are big business. The ability to express exogenous genes within eukaryotic cells is very popular and companies are falling over themselves marketing these products, claiming to ... read more
  • Effectene™ Transfection Reagent From Qiagen

    Effectene™ Transfection Reagent From Qiagen

    Wednesday, November 08, 2006
    Transfection reagents and protocols have become very widespread and utilized in many aspects of DNA research. There are many applications that require the introduction of vectors and expression ... read more
Page