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  • PEI - An Inexpensive Transfection Reagent

    PEI - An Inexpensive Transfection Reagent

    Tuesday, March 25, 2014
    My research involves making lentiviral vectors pseudotyped with vesicular stomatitis virus envelop glycoprotein (VSV-G). In this protocol, I transfect HEK-293T cells with 3 plasmids; one for ... read more
  • Antibody for HA Tagging a Protein

    Antibody for HA Tagging a Protein

    Friday, March 21, 2014
    Hemagglutinin (HA) epitope tag, or HA tag, is one of the most usual ways to mark and detect a protein in vivo. Good antibody for HA tag is essential to many popular laboratory techniques as WB, IF or ... read more
  • Loading Control for WB: Tubulin

    Loading Control for WB: Tubulin

    Monday, January 27, 2014
    Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins and a sixth ... read more
  • Alpha Amylase Detection in Pancreas

    Alpha Amylase Detection in Pancreas

    Friday, January 24, 2014
    a-Amylase is a protein enzyme EC 3.2.1.1 that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Pancreatic a-amylase randomly ... read more
  • Insulin Detection in Pancreas

    Insulin Detection in Pancreas

    Wednesday, December 25, 2013
    Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. Insulin causes cells in the liver, skeletal muscles, and... read more
  • Pancreas Metabolism by IHC-Paraffin

    Pancreas Metabolism by IHC-Paraffin

    Tuesday, December 24, 2013
    The main functions of pancreatic glucagon consist of gluconeogenesis, ketogenesis, lipolysis and glycogenolysis. Monoclonal anti-Glucagon antibody can be used as an analytical tool for ... read more
  • GFP Antibody for IF

    GFP Antibody for IF

    Monday, December 16, 2013
    Stress in vivo, and CRH application in vitro, cause dendritic spine loss in hippocampal pyramidal neurons. This loss of spines, and subsequent synapses, is likely the underlying mechanism through ... read more
  • ANTI-FLAG® M2 Affinity Gel from Sigma-Aldrich

    ANTI-FLAG® M2 Affinity Gel from Sigma-Aldrich

    Monday, March 11, 2013
    We had a system with cells stably overexpressing FLAG-tagged RBPs. By transfecting the small RNA duplexes and immunoprecipitating (IP’ing) the FLAG-tagged RBPs, we could then perform a Northern blot ... read more
  • Native Protein Deglycosylation Kit from Sigma-Aldrich

    Deglycosylation Kit from Sigma-Aldrich

    Tuesday, February 19, 2013
    The Native Protein Deglycosylation Kit is used to remove N-linked oligosaccharides from glycoproteins under native conditions as opposed to more traditional methods. Glycosylation protein ... read more
  • Rat/Mouse Growth Hormone ELISA Kit from EMD Millipore

    Growth Hormone ELISA Kit from EMD Millipore

    EMD Millipore
    Monday, February 04, 2013
    EMD Millipore’s Rat/Mouse Growth Hormone ELISA Kit offers a fast, simple and very sensitive way to measure growth hormone levels in living mice. It only requires 10 ul of serum and takes only 3.5 hrs ... read more
  • Monoclonal Anti-Desmin Antibody from Sigma-Aldrich, used in Western Blotting

    Monoclonal Anti-Desmin Antibody from Sigma-Aldrich, used in Western Blotting

    Tuesday, February 14, 2012
    In vivo, we analyzed regenerating skeletal muscle, otherwise desmin expression was very low; in vitro desmin is normally expressed by myogenic cell lines / primary cultures. Our results were quite ... read more
  • Rabbit Anti-Pan Cadherin Antibody from Sigma-Aldrich, used in Immunofluorescence

    Rabbit Anti-Pan Cadherin Antibody from Sigma-Aldrich, used in Immunofluorescence

    Tuesday, January 17, 2012
    I observed good staining in human colon epithelium cells and in smooth muscle cells (longitudinal and circular). read more
  • SYBR® Green JumpStart™ Taq ReadyMix™ from Sigma-Aldrich

    SYBR® Green JumpStart™ Taq ReadyMix™ from Sigma-Aldrich

    Wednesday, August 11, 2010
    Quantitative real-time PCR (qPCR) is fast replacing conventional reverse transcriptase PCR in many molecular biology labs, increasingly reflected by high quality journals adjusting their requirements ... read more
  • Molecular Weight Market Kit for Gel Filtration from Sigma-Aldrich

    Molecular Weight Market Kit for Gel Filtration from Sigma-Aldrich

    Wednesday, March 24, 2010
    Molecular weight markers for gel filtration are essential components for standardization of the column and for size prediction of an unknown sample. In gel filtration, the samples are run through a ... read more
  • GenElute Plasmid Prep Kit From Sigma-Aldrich

    GenElute Plasmid Prep Kit From Sigma-Aldrich

    Tuesday, September 29, 2009
    In the modern molecular world almost every biology lab today finds itself working with plasmid DNA in some capacity. One of the simplest tasks required is purifying plasmid DNA from small... read more
  • REDExtract-N-Amp™ Tissue PCR Kit From Sigma-Aldrich

    REDExtract-N-Amp™ Tissue PCR Kit From Sigma-Aldrich

    Wednesday, July 15, 2009
    REDExtract-N-Amp™ Tissue PCR Kit is a good kit in my opinion. I am a graduate student. My research involves characterizing the role of two proteins using an... read more
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