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  • HP1(alpha) Detection in Chromocenters

    HP1(alpha) Detection in Chromocenters

    Monday, June 02, 2014
    HP1 proteins bind to DNA regions to control gene expression and heterochromatin formation and maintenance. The amount of chromocenters detected by HP1 staining is a clear indication of alterations of ... read more
  • PEI - An Inexpensive Transfection Reagent

    PEI - An Inexpensive Transfection Reagent

    Tuesday, March 25, 2014
    My research involves making lentiviral vectors pseudotyped with vesicular stomatitis virus envelop glycoprotein (VSV-G). In this protocol, I transfect HEK-293T cells with 3 plasmids; one for ... read more
  • Antibody for HA Tagging a Protein

    Antibody for HA Tagging a Protein

    Friday, March 21, 2014
    Hemagglutinin (HA) epitope tag, or HA tag, is one of the most usual ways to mark and detect a protein in vivo. Good antibody for HA tag is essential to many popular laboratory techniques as WB, IF or ... read more
  • Loading Control for WB: Tubulin

    Loading Control for WB: Tubulin

    Monday, January 27, 2014
    Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins and a sixth ... read more
  • Alpha Amylase Detection in Pancreas

    Alpha Amylase Detection in Pancreas

    Friday, January 24, 2014
    a-Amylase is a protein enzyme EC 3.2.1.1 that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Pancreatic a-amylase randomly ... read more
  • Insulin Detection in Pancreas

    Insulin Detection in Pancreas

    Wednesday, December 25, 2013
    Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. Insulin causes cells in the liver, skeletal muscles, and... read more
  • Pancreas Metabolism by IHC-Paraffin

    Pancreas Metabolism by IHC-Paraffin

    Tuesday, December 24, 2013
    The main functions of pancreatic glucagon consist of gluconeogenesis, ketogenesis, lipolysis and glycogenolysis. Monoclonal anti-Glucagon antibody can be used as an analytical tool for ... read more
  • GFP Antibody for IF

    GFP Antibody for IF

    Monday, December 16, 2013
    Stress in vivo, and CRH application in vitro, cause dendritic spine loss in hippocampal pyramidal neurons. This loss of spines, and subsequent synapses, is likely the underlying mechanism through ... read more
  • Anti-TRA 1-60 Antibody from EMD Millipore

    Immunocytochemical Staining of ES and iPS Cells

    EMD Millipore
    Tuesday, May 21, 2013
    TRA-1-60 antigen is expressed upon the surface of human embryonic (ES) or human induced pluripotent stem (hiPS) cells. Therefore, it has been used as a marker of human ES and hiPS cells. In addition, ... read more
  • iVIEW™ DAB Detection Kit from Ventana

    iVIEW™ DAB Detection Kit from Ventana

    Wednesday, May 08, 2013
    I have used the iVIEW DAB Detection Kit to stain for the presence of various proteins in slides cut from formalin fixed paraffin embedded (FFPE) lymphoma tissue. These are then manually scored using ... read more
  • Bim Rabbit Monoclonal Antibody Clone Y36 from Abcam

    Anti-Bim Antibody Used in IHC

    Abcam
    Thursday, May 02, 2013
    Bim has been implicated in the pathobiology of diffuse large B-cell lymphoma (DLBCL). I am using the Bim antibody to measure Bim protein levels by immunohistochemistry (IHC) in DLBCL formalin fixed ... read more
  • c-Myc (9E10) Monoclonal Antibody from Santa Cruz Biotechnology

    c-Myc Antibody Used in IHC

    Santa Cruz Biotechnology, Inc.
    Tuesday, April 23, 2013
    c-Myc proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors, including diffuse large B-cell ... read more
  • ultraView Universal DAB Detection Kit from Ventana

    ultraView Universal DAB Detection Kit from Ventana

    Thursday, April 18, 2013
    The ultraView Universal DAB Detection Kit is specifically designed for performing immunohistochemistry (IHC) on the Ventana® NexES and BenchMark automated systems. The kit detects mouse IgG and IgM ... read more
  • Anti-Stage-Specific Embryonic Antigen-4 Antibody, clone MC-813-70 from EMD Millipore

    Anti-SSEA4 Antibody from EMD Millipore

    EMD Millipore
    Thursday, April 11, 2013
    SSEA4 antigen is expressed upon the surface of human embryonic stem (ES) cells and human induced pluripotent stem cells (iPSC). This antibody reacts with the stage-specific embryonic antigen-4 (SSEA-... read more
  • P21 Mouse Monoclonal Antibody Clone 4D10 from Abcam

    P21 Antibody from Abcam

    Abcam
    Wednesday, April 10, 2013
    The p21 protein is regulated by MYC and functions as a regulator of cell cycle progression. p21 expression is also controlled by the tumor suppressor protein p53, through which it mediates p53-... read more
  • ANTI-FLAG® M2 Affinity Gel from Sigma-Aldrich

    ANTI-FLAG® M2 Affinity Gel from Sigma-Aldrich

    Monday, March 11, 2013
    We had a system with cells stably overexpressing FLAG-tagged RBPs. By transfecting the small RNA duplexes and immunoprecipitating (IP’ing) the FLAG-tagged RBPs, we could then perform a Northern blot ... read more
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