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PEI - An Inexpensive Transfection Reagent
Tuesday, March 25, 2014
My research involves making lentiviral vectors pseudotyped with vesicular stomatitis virus envelop glycoprotein (VSV-G). In this protocol, I transfect HEK-293T cells with 3 plasmids; one for ...
Antibody for HA Tagging a Protein
Friday, March 21, 2014
Hemagglutinin (HA) epitope tag, or HA tag, is one of the most usual ways to mark and detect a protein in vivo. Good antibody for HA tag is essential to many popular laboratory techniques as WB, IF or ...
Loading Control for WB: Tubulin
Monday, January 27, 2014
Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins and a sixth ...
Alpha Amylase Detection in Pancreas
Friday, January 24, 2014
a-Amylase is a protein enzyme EC 126.96.36.199 that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen, yielding glucose and maltose. Pancreatic a-amylase randomly ...
Insulin Detection in Pancreas
Wednesday, December 25, 2013
Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. Insulin causes cells in the liver, skeletal muscles, and...
Pancreas Metabolism by IHC-Paraffin
Tuesday, December 24, 2013
The main functions of pancreatic glucagon consist of gluconeogenesis, ketogenesis, lipolysis and glycogenolysis. Monoclonal anti-Glucagon antibody can be used as an analytical tool for ...
GFP Antibody for IF
Monday, December 16, 2013
Stress in vivo, and CRH application in vitro, cause dendritic spine loss in hippocampal pyramidal neurons. This loss of spines, and subsequent synapses, is likely the underlying mechanism through ...
ANTI-FLAG® M2 Affinity Gel from Sigma-Aldrich
Monday, March 11, 2013
We had a system with cells stably overexpressing FLAG-tagged RBPs. By transfecting the small RNA duplexes and immunoprecipitating (IP’ing) the FLAG-tagged RBPs, we could then perform a Northern blot ...
Deglycosylation Kit from Sigma-Aldrich
Tuesday, February 19, 2013
The Native Protein Deglycosylation Kit is used to remove N-linked oligosaccharides from glycoproteins under native conditions as opposed to more traditional methods. Glycosylation protein ...
Monoclonal Anti-Desmin Antibody from Sigma-Aldrich, used in Western Blotting
Tuesday, February 14, 2012
, we analyzed regenerating skeletal muscle, otherwise desmin expression was very low;
desmin is normally expressed by myogenic cell lines / primary cultures. Our results were quite ...
Rabbit Anti-Pan Cadherin Antibody from Sigma-Aldrich, used in Immunofluorescence
Tuesday, January 17, 2012
I observed good staining in human colon epithelium cells and in smooth muscle cells (longitudinal and circular).
SYBR® Green JumpStart™ Taq ReadyMix™ from Sigma-Aldrich
Wednesday, August 11, 2010
Quantitative real-time PCR (qPCR) is fast replacing conventional reverse transcriptase PCR in many molecular biology labs, increasingly reflected by high quality journals adjusting their requirements ...
Molecular Weight Market Kit for Gel Filtration from Sigma-Aldrich
Wednesday, March 24, 2010
Molecular weight markers for gel filtration are essential components for standardization of the column and for size prediction of an unknown sample. In gel filtration, the samples are run through a ...
GenElute Plasmid Prep Kit From Sigma-Aldrich
Tuesday, September 29, 2009
In the modern molecular world almost every biology lab today finds itself working with plasmid DNA in some capacity. One of the simplest tasks required is purifying plasmid DNA from small...
REDExtract-N-Amp™ Tissue PCR Kit From Sigma-Aldrich
Wednesday, July 15, 2009
REDExtract-N-Amp™ Tissue PCR Kit is a good kit in my opinion. I am a graduate student. My research involves characterizing the role of two proteins using an...
SIGMAFAST™ BCIP®/NBT B5655 Tablets From Sigma-Aldrich
Wednesday, May 13, 2009
The technique of Western blotting is a laboratory staple of protein expression analysis. After loading protein samples onto an appropriate gel and separation by electrophoresis, the gel is then ...
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Rural Microbes Could Boost City Dwellers' Health
Thursday, April 24, 2014
The greater prevalence of asthma, allergies and other chronic inflammatory disorders among people of...
Not Just The Poor Live Hand-To-Mouth
Thursday, April 24, 2014
When the economy hits the skids, government stimulus checks to the poor sometimes follow.
Moffitt Cancer Center's Phase 3 Study May Be Game-Changer For Acute Myeloid Leukemia
Thursday, April 24, 2014
Moffitt Cancer Center researchers say clinical trials for a new experimental drug to treat acute ...
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