The BTX ECM630 is a pulse generator that can be used to introduce DNA, RNA, and a variety of other molecules into prokaryotic or eukaryotic cells of interest. The unit can also be used for cellular fusion techniques, as well as other procedures that require a controlled electrical pulse. Full regulation of voltage, capacitance and resistance coupled with an exponential decay system allows for unparalleled control over the time constant in the Precision Pulse System. As with most similar instruments, resistance can either be set by the user (up to 1575 ohms) or remain as ‘none’, in order to duplicate protocols from unit lacking a resistance control feature. As a result of this feature, the BTX630 can reproduce almost any electroporation conditions. In addition, the unit outperforms most competitors as it can deliver a voltage maximum of 6000A (max. deliverable voltage is 2500V) with a resolution of 1V up to 250V, and 5V, thereafter.
The set up of the ECM630 is fast and simple; the unit is plugged in, the electrode is attached, the power is turned on and the instrument is ready for operation. The main unit itself is quite small, measuring 12.5" x 12.3" of bench space and it is 5.5" in height. The ECM630 utilizes a backlit, 4-line digital LCD to display a menu-based system for interaction with the instrument. Similarly, the results and parameters reported following pulse delivery are easy to read and interpret. The instrument is controlled by only two buttons and one dial. The buttons are soft-touch buttons for turning the instrument on and off and for delivery of the pulse. The dial, or ‘rotary encoder’, allows the user to quickly and easily scroll through menus and change numerical parameters such as voltage, resistance etc. Pressing the dial confirms your selection.
Switching the unit on for the first time initiates a self-diagnostic test that is completed quickly. Immediately afterwards, a menu screen is displayed, allowing the adjustment of the basic parameters required for an electroporation experiment. Having entered the desired parameters using only the rotary encoder, the program may be saved for later use if required. By default, the unit displays the last-used program when next switched on. When the parameters are set, the electroporation cell is placed in the Perspex-covered electrode block. The provided electrode block contains fully width-adjustable electrodes and is also spring-loaded, guaranteeing a tight and conductive fit with the cell. Various alternative electrodes and chambers are available that are attached using banana plug sockets, hence any custom-designed cells can easily be adapted to the instrument. The cell is fully enclosed, providing a safety cut-off and protection during pulsing. When the cell is in place, the user simply presses the ‘pulse’ button on the main unit and display will indicate the status of the unit throughout the procedure. This takes a maximum of 20 seconds, including charging and pulsing but typically, the procedure takes a few seconds. After pulsing, the values for actual maximum pulse voltage and time constant are displayed automatically. The BTX-630 is protected from short circuit by safety features built into the unit.
The manual provided is thorough, with a quick start guide as well as more in-depth information about operation of the unit and theory behind electroporation and electrofusion technology. Sections on troubleshooting, recommended reading, a glossary and references are included. This unit has been in operation for several years and it has yet to show a fault.
Scott Coutts
Graduate Student
Department of Microbiology
Monash University
Australia