Biolegend
CD152 BV421
106312
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Browse Similar Products: CD152 Flow Cytometry Antibodies
My research aim is to investigate the response of the immune system after immune-therapy. This antibody has been used to investigate changes in CTLA4 expression on lymphocytes extracted from spleen, lymph nodes and glioma tumor-bearing mice.
Flow Cytometry
Glioma tumor, mice
30min
Fc
N/A
Fortessa
The experiment was performed to show change in the expression of CTLA4 in brain tumors of treated mice. Cells were gated for Live/CD45/CD3+, Live/CD45/CD3+/CD8+, Live/CD45/CD3+/CD4+After cell extraction and blocking with Fc Block, wash your sample 2x with washing buffer, stain for 30min RT with your extracellular staining, wash 2x and then proceed with intracellular staining protocol for CTLA4 (1:100).
Little background
Not a high signal
Good clone and antibody to evaluate CTLA4 expression ex vivo.