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Alexa Fluor® 488 anti-human IgD Antibody
348216
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The general aim of our research is to characterize the phenotype and function of pathogenic B cells in various diseases. We are building fluorescence and mass cytometry panels to study human B cells in the context of autoimmune and idiopathic pulmonary diseases. The main reason I selected IgD-AF488 was because it fit well in my 14-parameter panel with minimal compensation issues.
Flow Cytometry
Cryopreserved human PBMC
0.5uL (1:400) IgD-AF488 in 200uL FACS buffer with 2x10^6 cells; 15 minutes; room temperature
None
BD LSRII 5-laser Flow Cytometer
A single color titration of IgD-AF488 was performed and 0.5uL (1:400) yielded the highest stain index. We routinely use IgD-AF488 in our 14-parameter human B cell panel to identify B cells in patient samples with various diseases. Cyropreserved human PBMCs are thawed, washed twice in PBS, and stained with a cocktail of surface antibodies, including IgD-AF488. ~2 million cells in 200uL FACS buffer are incubated with antibodies for 15 minutes at room temperature.As evidenced in the attached figure, the staining looks great. It detects the expected frequency of IgD+ B cells in human PBMCs.
N/A
Staining pattern is good; good price
Antibody works great!