Biolegend
Brilliant Violet 785 anti-human CD45RA Antibody
304139
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: CD45RA Flow Cytometry Antibodies
The aim of my research is to observe how HIV infection impacts on T cell activation. The goal of this experiment was to observe how stimulation in vitro alone (CD3/CD28 Abs) affects memory phenotype in human CD4+ T cells. I chose this product as Biolegend offers good quality antibodies at good price.
Flow Cytometry
Human PBMC (CD4+ isolated)
1:100 (50 ul), 30min, 4deg
NA
BD Fortessa-X20
We can observe increased percentage of CD4+ T cells in the memory compartment (CD45RA-) after 3 day (in vitro) stimulation with CD3/CD28 antibodies.
Good staining at high dilution
None
I would recommend this product for flow cytometry.