Excellent CD38 Staining On Human PBMCs

November 27, 2017

Infection and Immunity
University College London
PhD Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PerCP anti-human CD38 Antibody

Catalog Number:

303519

The aim of this study was to observe activation markers upon CD3/CD28 stimulation of purified human CD4+ T cells. I selected this product as Biolegend offers high quality antibodies at good prices.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMC (CD4+ isolated)

Primary Incubation

1:150 (50 ul), 30min, 4deg

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD Fortessa-X20

Results Summary

We can observe good upregulation of CD38 and HLA-DR expression after 3 days of CD3/CD28 stimulation.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Good staining at high dilution

The Bad

None

The Bottom Line

I would recommend this antibody for flow cytometry.

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