Use Of Mouse CD4+ T Cell Enrichment Column For In Vitro Culture Studies

November 29, 2017

University of Nebraska Medical Center
Pharmacology and Experimental Neuroscience
Graduate Research Assistant

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

R&D Systems/ Novus Biologicals - Bio-Techne Brands

Product Name:

Mouse CD4+ T Cell, Small Column Enrichment Kit

Catalog Number:

MCD4C-1000

The R&D Systems Mouse CD4+ T Cell Enrichment Column was used to generate a 90% or greater purity CD4+ T cell population that was used in vitro to polarize the T cells to a specific T effector cell phenotype.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

C57BL/6 mouse lymph node and splenic cells

Protocol Overview

Spleens and lymph nodes from C57BL/6 mice were collected, turned into a single cells suspension, and then run through the R&D Systems Mouse CD4+ T Cell Enrichment Column in order to generate a 90% plus CD$+ T cell population. This population was then cultured in vitro for 5 days with a milieu of cytokines and antibodies in order to generate either a Th1 or Th17 skewed phenotype.

Tips

Do not mix the lymph node and splenic cells together until after the column as it will cause clumping of your cells.

Results Summary

The column generates 90% or more pure population of CD4+ T cells every time it is used.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

This kit is very consistent in its results and extremely easy to use.

The Bad

This particular column utilizes negative selection in order to gain a pure population instead of positive selection.

The Bottom Line

This is a great kit to use to gain a pure population of CD4+ T cells as it is relatively inexpensive, and very easy to use.

Join the discussion