Mouse CD11c PE-Cy7 Antibody

November 15, 2017

Medicine
University of Colorado Denver
Professional Research Assistant

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies

Product Name:

CD11c Monoclonal Antibody (N418), PE-Cyanine7, eBioscience™

Catalog Number:

25-0114-82

This antibody was used in a flow cytometry (FACS) panel to detect various lymphocyte populations (mainly alveolar macrophages and eosinophils) in murine bronchoalveolar lavage.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine brochoalveolar lavage

Primary Incubation

1:200 in FACS buffer, 30 mins at 4C or on ice covered, or in dark

Blocking Agent

PBS, 1% BSA & 0.05% NaN3

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This antibody worked great and produced low background staining in FACS analysis of cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Highly specific staining, low background

The Bad

Unclear if it works for applications other than FACS

The Bottom Line

Great product, produces highly specific staining with low background in FACS applications. Reasonably priced.

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