Biolegend
FITC anti-human CD27 antibody
302806
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Browse Similar Products: CD27 Flow Cytometry Antibodies
This antibody has been used in a flow cytometry based study immunophenotyping PBMC populations obtained from cancer patients. The results have not yet been published. Multi-colour antibody panels were designed to identify a number of lymphocyte populations, and tested to ensure appropriate compensation was possible. CD27 was used to identify a memory population of B cells.
Flow Cytometry
Human peripheral blood mononuclear cells (PBMC)
5uL FITC-CD27 for 30 minutes at 4oC
FcR blocking reagent, human (Miltenyi Biotec, cat# 130-059-901)
NA
Beckman Coulter Galios, Kaluza software
Clear staining that allowed the identification of memory B cells. Fit well into an eight colour panel with no compensation issues.
Clear staining and useful for FACS analysis.
The antibody has only been tested for flow cytometry
Easy to use antibody pack that provided clear and distinct staining of B lymphocyte sub-population.