CD19 Antibody

Pediatrics
Medical College of Wisconsin
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-human CD19 Antibody

Catalog Number:

363006

This antibody has been used to detect CD19 expression on primary B cells, and to confirm CD19 expression in cells transduced with lentivirus to express CD19.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary human B cells, CD19+ human cell lines

Primary Incubation

1:100, 30 minute incubation at 4 degrees

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

This antibody gives a distinct peak of CD19+ cells easily differentiated from CD19- cells

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very effective for flow cytometry

The Bad

The stated dilution (5uL per test) is far more antibody than is actually required. Titrate for your particular use.

The Bottom Line

This is a very effective antibody

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