Very Good CD11c APC Antibody

October 20, 2017

Internal Medicine II
University of Tübingen
PhD Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Miltenyi Biotec

Product Name:

CD11c-APC, human

Catalog Number:

130-092-412

In order to identify if my culture of human monocyte derived DCs is pure, I used this CD11c APC antibody in addition with an HLA-DR BV650 staining. I selected this product, as it is easy to use and cost-effective compared to other products from other companies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Cultured human monocyte derived DCs

Primary Incubation

1:20 dilution (5ul in 100ul), 30 min at 4°C

Blocking Agent

IgG from human serum (from Sigma) for 20 min at RT

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

After gating on Single cells - Lymphocytes - living cells, it is very easy to gate on CD11c+ cells, as the resolution of the antibody is very good.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very easy to use, no predilution is needed

The Bad

None

The Bottom Line

The product is very good to identify cultured human monocyte derived DCs.

Share your experience with other scientists. Write a Review! »

Join the discussion