Bright Fixable Live/Dead Marker

October 18, 2017

University of California, Merced
Molecular and Cell Biology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Fixable Viability Dye eFluor® 780 100 tests

Catalog Number:

65-0865-14

This stain can be detected on a red laser with a 780/60 bandpass filter. It fits well in complex flow cytometry staining panels with only modest compensation requirements. It maintains a bright and distinct stain in various fixation procedures. Used at a dilution this live/dead marker can be well worth the cost.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

Murine Splenocytes

Protocol Overview

Stain up to 3x10^6 cells in 50ul of PBS with 1% FBS. Use the Fixable Viability dye eFluor 780 in an antibody cocktail at a 1:1000 dilution for 30 minutes at 4 degrees Celcius. Wash samples and proceed with desired fixation and any subsequent staining steps.

Tips

It may be able to titrate out more than 1:1000 but this gives a clear distinct population.

Results Summary

It will maintain a robust stain despite fixation or numerous washes. Dead cells are easily visible using this dye.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Distinct staining of dead cells with only modest compensation.

The Bad

Can be more expensive than other live/dead markers

The Bottom Line

If you are working in a complex panel or you are fixing your cells this dye works well as a live/dead marker with minimal optimization required.

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