Anti-Mouse CD8a (BV510) Antibody

October 11, 2017

Cell Biology
La Jolla Institute for Allergy and Immunology
Instructor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 510™ anti-mouse CD8a Antibody

Catalog Number:

100752

The aim was to study T helper cell subsets. The specific goal was to examine CD8 T cells from mouse splenocytes. I selected this product because it was of high quality and is cost effective.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 (0.4ul of antibody in 40ul of sample)

Blocking Agent

5% rat serum

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Brilliant Violet 510 (BV510)

Results Summary

This antibody was working well for detecting CD8 T cells from mouse tissues.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

High quality

The Bad

None

The Bottom Line

I would recommend this product for flow cytometry.

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