Invitrogen Antibodies
FOXP3 Monoclonal Antibody (FJK-16s), PE, eBioscience™
12-5773-82
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Browse Similar Products: FOXP3 Flow Cytometry Antibodies
The aim was to study T helper cell subsets. The specific goal was to examine Foxp3+ regulatory T cells from mouse CD4 T cells. I selected this product because it was of high quality.
Flow Cytometry
Mouse splenocytes
1:100 (0.4ul of antibody in 40ul of sample)
1X Permeabilization buffer (ThermoFisher)
NA
PE channel
Treg cells were determined by Foxp3 expression using this antibody
Specificity
Fixation/Permeabilization is required
For best result, the staining buffer set (ThermoFisher Scientific, Cat no. 00-5523-00) was required.This buffer set includes both Fixation and permeabilization buffers.