Culture Of Monolayer Adherent Glioma Stem Cells In Laminin Coated Plates

indian institute of chemical technology
chemical biology

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Company:

Thermo Fisher Scientific

Product Name:

Natural mouse laminin

Catalog Number:

23017015

The aim is to culture the GBM stem cell spheres as adherent monolayer culture. This product (laminin) supports the growth of GBM stem cells as a monolayer upon coating with it on cell culture dishes.

Experimental Design and Results Summary

Application

Adherent monolayer culture of GBM stem cells or for the differentiation

Starting Material

GBM stem cells, laminin coated plates

Protocol Overview

Coat with laminin (10µg/ml) for 3h @ 370C or O/N @ 2-80C, Give one 1XPBS wash and plate the cells or store with 1x DPBS

Tips

Do not allow the plates to get dry and they should be used within the same day of coating

Results Summary

The laminin coated plates have supported the growth of monolayer culture of GBM stem cells

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

It helped the adherent culture of GBM stem cells

The Bad

No negative remarks

The Bottom Line

Recommended for the culture of GBM stem cells as adherent monolayer culture and differentiation work.

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