Vector Laboratories
Peroxidase Substrate Kit DAB
SK 4100
Info:Supplier Page View Product Specs View Product
The aim of the research was to analyze the colocalization of TGFb1 in the pancreatic compartment of mouse tissue section. In IHC, the pancreatic tissue sections were incubated with TGFb1 primary antibody and a biotin labeled secondary antibody followed by streptavidin. Finally, DAB was used and brown color indicated TGFb1 staining.
Immunohistochemical analysis
Paraffin embedded mouse tissue sections
Mix the solution provided in 4 different bottles as per instruction of manual. DAB is a mutagenic compound and should be handled with care, using gloves and the appropriate protective gear.
Prepare fresh before the use
3,3′-Diaminobenzidine (DAB), a derivative of benzene, most commonly for immunohistochemical procedures. It gives brown color when used with ABC kit.
N/A
Preparation of DAB takes 2 min time and can be made quickly before the use.
NA
It's a good peroxidase substrate kit for immunohistochemical analysis and sometimes can also be used in immunoblot analysis.